KR970061272A - Purification method of hepatitis B surface antigen - Google Patents

Purification method of hepatitis B surface antigen Download PDF

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Publication number
KR970061272A
KR970061272A KR1019960002532A KR19960002532A KR970061272A KR 970061272 A KR970061272 A KR 970061272A KR 1019960002532 A KR1019960002532 A KR 1019960002532A KR 19960002532 A KR19960002532 A KR 19960002532A KR 970061272 A KR970061272 A KR 970061272A
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KR
South Korea
Prior art keywords
hbs antigen
antigen
silica
hbs
hepatitis
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Application number
KR1019960002532A
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Korean (ko)
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KR0159716B1 (en
Inventor
임국진
김국현
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성재갑
주식회사 Lg 화학
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Priority to KR1019960002532A priority Critical patent/KR0159716B1/en
Publication of KR970061272A publication Critical patent/KR970061272A/en
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Publication of KR0159716B1 publication Critical patent/KR0159716B1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/29—Hepatitis virus
    • A61K39/292—Serum hepatitis virus, hepatitis B virus, e.g. Australia antigen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 B형 간염 표면 항원(HBs 항원)을 정제하는 방법에 관한 것으로, HBs 항원이 발현된 효모 세포를 파쇄하여 세포 파쇄액을 얻고, 상기 파쇄액을 원심분리하여 HBs 항원을 함유하는 상등액을 얻은 다음, 상기 상등액에 중성 비이온 계면활성제 및 실리카를 가하여 HBs 항원을 흡착시킨 후, HBs 항원이 흡착된 실리카로부터 HBs 항원을 탈착시킴으로써 HBs 항원을 고속도로 정제할 수 있다.The present invention relates to a method for purifying a hepatitis B surface antigen (HBs antigen), which comprises disrupting a yeast cell expressing HBs antigen to obtain a cell lysate, centrifuging the above-mentioned disruption liquid and culturing a supernatant containing HBs antigen HBs antigen can be purified at high speed by adsorbing HBs antigen by adding a neutral nonionic surfactant and silica to the supernatant and desorbing HBs antigen from the silica adsorbed by the HBs antigen.

Description

B형 간염 표면 항원의 정제방법Purification method of hepatitis B surface antigen

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

Claims (4)

B형 간염 표면 항원(HBs 항원)이 발현된 효모 세포를 파쇄하여 세포 파쇄액을 얻고, 상기 파쇄액을 원심분리하여 HBs 항원을 함유하는 상등액을 얻은 다음, 상기 상등액에 중성 비이온 계면활성제 및 실리카를 가하여 HBs 항원을 흡착시킨 후, HBs 항원이 흡착된 실리카로부터 HBs 항원을 탈착시키는 단계를 포함하는, B형 간염 표면 항원의 정제 방법.A yeast cell expressing hepatitis B surface antigen (HBs antigen) was disrupted to obtain a cell lysate, and the resulting lysate was centrifuged to obtain a supernatant containing HBs antigen. To the supernatant was added a neutral nonionic surfactant and silica To adsorb the HBs antigen, and then desorbing the HBs antigen from the silica to which the HBs antigen has been adsorbed. 제1항에 있어서, 상기 계면활성계가 0.05 내지 0.5%의 농도로 첨가되는 것을 특징으로 하는 방법.The method according to claim 1, wherein the surfactant is added in a concentration of 0.05 to 0.5%. 제1항에 있어서, 카보네이트 또는 보레이트 완충용액에서 상기 HBs 항원이 흡착된 실리카로붙 HBs 항원을 탈착시키는 것을 특징으로 하는 방법.2. The method of claim 1, wherein said HBs antigen desorbs the bound HBsA antigen with silica adsorbed in a carbonate or borate buffer solution. 제3항에 있어서, 사기 완충 용액의 pH가 8.8 내지 11.0인 것을 특징으로 하는 방법.The method according to claim 3, wherein the pH of the scavenging buffer is 8.8 to 11.0. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960002532A 1996-02-02 1996-02-02 Process for the purification of hepatitis b surface antigen KR0159716B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960002532A KR0159716B1 (en) 1996-02-02 1996-02-02 Process for the purification of hepatitis b surface antigen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960002532A KR0159716B1 (en) 1996-02-02 1996-02-02 Process for the purification of hepatitis b surface antigen

Publications (2)

Publication Number Publication Date
KR970061272A true KR970061272A (en) 1997-09-12
KR0159716B1 KR0159716B1 (en) 1998-12-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019960002532A KR0159716B1 (en) 1996-02-02 1996-02-02 Process for the purification of hepatitis b surface antigen

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2015114330A (en) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. CHROMATOGRAPHIC MEDIA AND DEVICES
CN117042805A (en) 2021-04-05 2023-11-10 株式会社Lg化学 Vaccine composition against coronavirus

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