KR970058725A - Methods for quantitative determination of antibody titers against hepatitis C virus - Google Patents

Methods for quantitative determination of antibody titers against hepatitis C virus Download PDF

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Publication number
KR970058725A
KR970058725A KR1019960000791A KR19960000791A KR970058725A KR 970058725 A KR970058725 A KR 970058725A KR 1019960000791 A KR1019960000791 A KR 1019960000791A KR 19960000791 A KR19960000791 A KR 19960000791A KR 970058725 A KR970058725 A KR 970058725A
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KR
South Korea
Prior art keywords
virus
antibody
hrp
hepatitis
solid support
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KR1019960000791A
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Korean (ko)
Inventor
소홍섭
류왕식
조중명
박영민
김부성
Original Assignee
성재갑
주식회사 Lg 화학
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Application filed by 성재갑, 주식회사 Lg 화학 filed Critical 성재갑
Priority to KR1019960000791A priority Critical patent/KR970058725A/en
Publication of KR970058725A publication Critical patent/KR970058725A/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 HCV 항체 역가를 정량적으로 측정하는 방법 및 이를 위한 진단 시약에 관한 것으로, HCV의 핵항원과 제3비구조 단백질의 융합 단백질 항원을 고체지지체에 흡착시키고, 분석할 시료 혈청을 연속적으로 회석하여 상기 고체지지체에 가하여 반응시키고, 서양고추냉이 과산화효소(HRP)가 접합된 감마 면역글로불린 항체(Anti-human IgG-HRP)를 가하여 반응시키고, 발색과정을 거쳐 항체의 양을 시료배수에 따라 정량화시키는 것을 특징으로 하는 C형 감염 바이러스에 대한 항체 역가의 정량적 측정 방법은, C형 간염환자의 치료방법의 선택 및 치료경과 등의 판단에 유용하게 사용될 수 있다.The present invention relates to a method for quantitatively measuring the activity of an HCV antibody and a diagnostic reagent for the same, wherein a fusion protein antigen of the nuclear antigen and the third non-structural protein of HCV is adsorbed on a solid support, The resulting mixture was reacted with the solid support, reacted with anti-human IgG-HRP conjugated with horseradish peroxidase (HRP), and the amount of the antibody was quantitated according to the sample drainage A method for quantitatively measuring the antibody titer against the C-type infectious virus can be usefully used for the selection of the treatment method for the hepatitis C patient and the judgment of the treatment progress.

Description

C형 간염 바이러스에 대한 항체의 정량적 역가 측정 방법Methods for quantitative determination of antibody titers against hepatitis C virus

내용없음No content

Claims (2)

C형 간염 바이러스 항체 역가의 정량적 측정을 위한, C형 간염 바이러스의 핵항원과 제3비구조 단백질의 융합 단백질 항원을 포함하는 진단 시약.A diagnostic reagent comprising a nuclear antigen of hepatitis C virus and a fusion protein antigen of a third nonstructural protein for quantitative measurement of antibody titers of hepatitis C virus. C형 간염 바이러스의 핵항원과 제3비구조 단백질의 융합 단백질 항원을 고체지지체에 흡착시키고, 분석할 시료 혈청을 연속적으로 회석하여 상기 고체지지체에 가하여 반응시키고, 서양고추냉이 과산화효소(horseradish peroxidase; HRP)가 접합된 감마 면역글로불린 항체(Anti-human IgG-HRP)를 가하여 반응시키고, 발색과정을 거쳐 항체의 양을 시료배수에 따라 정량화시키는 것을 특징으로 하는, C형 간염 바이러스에 대한 항체 역가의 정량적 측정 방법.The fusion protein antigen of the hepatitis C virus nuclear antigen and the third nonstructural protein is adsorbed on a solid support, the sample serum to be analyzed is continuously subjected to the reaction by adding thereto the solid support and reacting with horseradish peroxidase. HRP) conjugated with anti-human IgG-HRP, and quantitating the amount of the antibody after color development according to the sample drainage. The anti-human IgG-HRP antibody Quantitative measurement method. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960000791A 1996-01-17 1996-01-17 Methods for quantitative determination of antibody titers against hepatitis C virus KR970058725A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960000791A KR970058725A (en) 1996-01-17 1996-01-17 Methods for quantitative determination of antibody titers against hepatitis C virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960000791A KR970058725A (en) 1996-01-17 1996-01-17 Methods for quantitative determination of antibody titers against hepatitis C virus

Publications (1)

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KR970058725A true KR970058725A (en) 1997-08-12

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KR1019960000791A KR970058725A (en) 1996-01-17 1996-01-17 Methods for quantitative determination of antibody titers against hepatitis C virus

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