KR970028549A - Method for quantifying creatine kinase and reagents thereof - Google Patents

Abstract

It is an object of the present invention to provide a method for stably measuring creatine kinase stably, and to provide a creatine kinase quantitative reagent having improved shelf life in the form of a liquid. In other words, the present invention provides a method for measuring the activity of creatine kinase by activating creatine kinase in a sample with a trivalent compound and sulfhydryl in an aqueous medium, and stabilizing sulfhydryl by coexisting a trivalent compound with sulfhydryl. And a creatine kinase quantitative reagent comprising a reactive substance of a trivalent compound, sulfurhydryl and creatine kinase.

Description

Method for quantifying creatine kinase and reagents thereof

Since this is an open matter, no full text was included.

Claims (16)

Coexisting creatine kinase in the sample with trivalent phosphorus and sulfhydryl-containing compounds in an aqueous medium to activate creatine kinase, measuring the activity of the activated creatine kinase, and measuring the activity of the activated creatine kinase. Step of quantifying creatine kinase. The method for quantifying creatine kinase according to claim 1, wherein the sulfhydryl-containing compound is N-acetylcysteine. The method for quantifying creatine kinase according to claim 1, wherein the sulfhydryl-containing compound is N-acetylcysteine. The method of claim 1, wherein the aqueous medium is a buffer. A method for stabilizing a sulfhydryl-containing compound in which a trivalent phosphorus compound and a sulfhydryl-containing compound coexist in an aqueous medium. The method of claim 5, wherein the trivalent phosphorus compound is selected from substituted or unsubstituted phosphines and substituted or unsubstituted diphosphane. The method of stabilizing a sulfhydryl-containing compound according to claim 5, wherein the sulfhydryl-containing compound is N-acetylcysteine. 6. The method of claim 5, wherein the aqueous medium is a buffer. A creatine kinase assay reagent consisting of trivalent phosphorus compounds, sulfhydryl-containing compounds and reactive substances of creatine kinase. The creatine kinase quantitative reagent according to claim 9, wherein the trivalent phosphorus compound is selected from substituted or unsubstituted phosphine and substituted or unsubstituted diphosphane. The creatine kinase quantitative reagent according to claim 9, wherein the sulfhydryl-containing compound is N-acetylcysteine. The creatine kinase quantitative reagent according to claim 9, further comprising a buffer. Kit for quantitative determination of creatine kinase consisting of a trivalent phosphorus compound, a sulfhydryl-containing compound, and a reactive substance of creatine kinase. The kit for quantitative determination of creatine kinase according to claim 13, wherein the trivalent phosphorus compound is selected from substituted or unsubstituted phosphine and substituted or unsubstituted diphosphane. The kit for quantitative determination of creatine kinase according to claim 13, wherein the sulfhydryl-containing compound is N-acetylcysteine. The kit for quantitative determination of creatine kinase according to claim 13, further comprising a buffer. ※ Note: The disclosure is based on the initial application.