KR970027301A - Enzyme pullulanase produced from Thermus caldophilus GK-24 - Google Patents

Enzyme pullulanase produced from Thermus caldophilus GK-24 Download PDF

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Publication number
KR970027301A
KR970027301A KR1019950043764A KR19950043764A KR970027301A KR 970027301 A KR970027301 A KR 970027301A KR 1019950043764 A KR1019950043764 A KR 1019950043764A KR 19950043764 A KR19950043764 A KR 19950043764A KR 970027301 A KR970027301 A KR 970027301A
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South Korea
Prior art keywords
pullulanase
enzyme
enzyme pullulanase
thermus caldophilus
produced
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KR1019950043764A
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Korean (ko)
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KR100186901B1 (en
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김철호
고정헌
이영춘
이대실
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김은영
한국과학기술연구원
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Publication of KR970027301A publication Critical patent/KR970027301A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 극호열성 균주인 더머스 칼도필러스(Thermus caldopilus) GK-24로부터 유래된 풀루란아제(pullulanase), 그의 제조방법과 탄수화물 가수분해효소로서의 용도에 관한 것이다.The present invention relates to a pullulanase derived from the extremely thermophilic strain Thermus caldopilus GK-24, a preparation method thereof, and a use as a carbohydrate hydrolase.

Description

더머스 칼도필러스(Thermus caldophilus) GK-24로부터 생산되는 효소 풀루란아제(pullulanase)Enzyme pullulanase produced from Thermus caldophilus GK-24

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 풀루란아제의 정제과정을 나타내는 크로마토그램이다.1 is a chromatogram showing the purification process of pullulanase.

제2도는 정제한 풀루란아제의 SDS-변성 폴리아크릴아미드겔 전기영동 사진이다. 큰 화살표는 정제된 풀루란아제의 위치를 표시한 것이고 작은 화살표는 표준 단백질을 표시한 것이다.2 is an electrophoretic photograph of SDS-modified polyacrylamide gel of purified pullulanase. Large arrows indicate the location of purified pullulanase and small arrows indicate standard proteins.

제3도는 등전점을 확인하는 IEF의 결과 사진이다.3 is a photograph of the resulting IEF identifying the isoelectric point.

Claims (7)

Thermus 속 유래의 효소 풀루란아제(pullulanase).Enzyme pullulanase from the genus Thermus. 제1항에 있어서, 더머스 칼도필러스 GK-24(Thermus caldophilus G-24)로부터 생산되는 것을 특징으로 하는 효소 풀루란아제.The enzyme pullulanase of claim 1, wherein the enzyme pullulanase is produced from Dermus caldophilus G-24. 제1항 또는 제2항에 있어서, 풀루란, α,β-제한 덱스트린(α,β-limited dextrin), β-제한 덱스트린(β-limited dextrin), 가용성 녹말, 아밀로펙틴, 감자녹말 그리고 글리코겐을 가수분해하는 것을 특징으로 하는 효소 풀루란아제.The method according to claim 1 or 2, wherein pullulan, α, β-limited dextrin, β-limited dextrin, soluble starch, amylopectin, potato starch and glycogen are synthesized. Enzyme pullulanase, characterized in that the decomposition. 제2항에 있어서, 분자량이 64 kDa인 것을 특징으로 하는 효소 풀루란아제.The enzyme pullulanase according to claim 2, wherein the molecular weight is 64 kDa. 제2항에 있어서, 풀루란아제의 아미노 말단의 서열이 Ala-Pro-Gln-Asp-Asn-Ser-Leu-Xaa-Ile-Gly-Ala-(Ser)인 것을 특징으로 하는 풀루란아제.The pullulanase according to claim 2, wherein the amino terminal sequence of the pullulanase is Ala-Pro-Gln-Asp-Asn-Ser-Leu-Xaa-Ile-Gly-Ala- (Ser). 제2항에 있어서, pH 5.0∼6.0에서 최대의 활성을 나타내는 것을 특징으로 하는 효소 풀루란아제.The enzyme pullulanase according to claim 2, wherein the enzyme pullulanase exhibits maximum activity at pH 5.0 to 6.0. 제2항에 있어서, 70∼80℃에서 최대의 활성을 나타내는 것을 특징으로 하는 풀루란아제.The pullulanase according to claim 2, which exhibits maximum activity at 70 to 80 ° C. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950043764A 1995-11-25 1995-11-25 Pullulanase produced from thermus caldophilus gk-24 KR100186901B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950043764A KR100186901B1 (en) 1995-11-25 1995-11-25 Pullulanase produced from thermus caldophilus gk-24

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950043764A KR100186901B1 (en) 1995-11-25 1995-11-25 Pullulanase produced from thermus caldophilus gk-24

Publications (2)

Publication Number Publication Date
KR970027301A true KR970027301A (en) 1997-06-24
KR100186901B1 KR100186901B1 (en) 1999-04-01

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