KR970020110A - Biodegradable polysaccharide sponge containing tissue growth factor for osteoblast transplantation to restore periodontal tissue - Google Patents

Biodegradable polysaccharide sponge containing tissue growth factor for osteoblast transplantation to restore periodontal tissue Download PDF

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Publication number
KR970020110A
KR970020110A KR1019970003984A KR19970003984A KR970020110A KR 970020110 A KR970020110 A KR 970020110A KR 1019970003984 A KR1019970003984 A KR 1019970003984A KR 19970003984 A KR19970003984 A KR 19970003984A KR 970020110 A KR970020110 A KR 970020110A
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South Korea
Prior art keywords
growth factor
tissue growth
sponge
tissue
preparation
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KR1019970003984A
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Korean (ko)
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KR100231279B1 (en
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정종평
이승진
박윤정
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정종평
이승진
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Priority to KR1019970003984A priority Critical patent/KR100231279B1/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/12—Phosphorus-containing materials, e.g. apatite

Abstract

본 발명은 치주조직 복원을 위한 골아세포 이식용 제제에 관한 것으로, 상세하게는 키토산으로 제조된 스폰지(sponge)에 골아세포 및 조직 성장인자가 함유되어 있는 치주조직 복원을 위한 생분해성 세포이식용 제제에 관한 것이다.The present invention relates to a preparation for osteoblast transplantation for restoration of periodontal tissue, and more particularly to a biodegradable cell transplant preparation for restoration of periodontal tissue containing osteoblasts and tissue growth factors in a sponge made of chitosan. It is about.

본 발명은 키토산으로 이루어진 스폰지의 표면을 트리폴리인산 나트륨으로 가교시키고, 그 내부에 조직 성장 인자 또는 무기물을 함유시켜 골아세포를 부착증식시킴으로써, 골아세포의 이식에 이용될 수 있도록 한 것을 특징으로 한다.The present invention is characterized in that the surface of the sponge made of chitosan is crosslinked with sodium tripolyphosphate, and the tissue growth factor or inorganic material is contained therein so as to attach and proliferate osteoblasts, so that it can be used for transplantation of osteoblasts.

본 발명은 스폰지를 구성하는 키토산에 의해 세포가 부착되고 함유된 무기물 또는 조직 성장인자가 서서히 방출되어 세포가 증식·분화할 수 있게 하며, 분화후에는 생체내 리소자임에 의해 스폰지는 완전히 분해된다.In the present invention, the cells are attached by chitosan constituting the sponge, and the inorganic or tissue growth factors contained therein are gradually released to allow the cells to proliferate and differentiate. After the differentiation, the sponge is completely decomposed by lysozyme in vivo.

Description

치주조직을 복원하기 위한 골아세포 이식용 조직 성장인자를 함유한 생분해성 폴리사카리이드 스폰지제제Biodegradable polysaccharide sponge containing tissue growth factor for osteoblast transplantation to restore periodontal tissue

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 실시예 1에 의해 제조된 키토산 스폰지(sponge)의 시차주사 현미경 사진부도,1 is a differential scanning micrograph of the chitosan sponge prepared in Example 1,

제2도는 실시예 1에 의해 제조된 스폰지의 수분 함유율을 나타낸 그래프,2 is a graph showing the water content of the sponge prepared in Example 1,

제3도는 실시예 1에 의해 제조된 스폰지의 생체의 분해율을 나타낸 그래프,3 is a graph showing the decomposition rate of the living body of the sponge prepared in Example 1,

제4도는 실시예 1에 의해 제조된 스폰지로부터 혈소판유래 성장인자의 용출양상을 나타내는 그래프,4 is a graph showing the dissolution patterns of platelet-derived growth factors from the sponge prepared in Example 1,

제5도는 실시예 2에 의해 제조된 스폰지로부터의 혈소판유래 성장인자의 용출양상을 나타내는 그래프,5 is a graph showing the dissolution patterns of platelet-derived growth factors from the sponge prepared in Example 2,

제6도는 실시에 3에 의해 제조된 스폰지의 시차주사 현미경 사진부도,6 is a differential scanning micrograph of the sponge prepared in Example 3,

제7도는 본 발명에 따라 제조된 시폰지에 대한 골아세포의 접착력을 나타내는 그래프,7 is a graph showing the adhesion of osteoblasts to the chiffon prepared according to the present invention,

제8도는 본 발명에 따라 제조된 스폰지에 대한 골아세포의 부착양상을 나타내는 시차주사의 현미경 사진부도.8 is a micrograph of the differential scanning showing the appearance of osteoblast adhesion to the sponge prepared according to the present invention.

Claims (5)

치주조직 복원을 위한 골아세포 이식용 제제에 있어서, 상기 제제는 그 표면이 트리폴리인산 나트륨으로 가교된 키토산으로 이루어져 있으며, 그 내부에 조직 성장인자가 봉입된 것을 특징으로 하는 스폰지형 제제.A preparation for osteoblast transplantation for periodontal tissue restoration, wherein the preparation comprises a chitosan cross-linked with sodium tripolyphosphate and a tissue growth factor is enclosed therein. 청구항 1에 있어서, 상기 제제는 인회석 또는 인산삼석회를 또한 함유하는 것을 특징으로 하는 스폰지형.The sponge type according to claim 1, wherein the preparation further contains apatite or tricalcium phosphate. 청구항 1에 있어서, 상기 조직 성장인자는 혈소판유래 조직 성장인자, 인슐린유사 조직 성장인자, 상피세포 성장인자, 섬유아세포 성장인자 및 변형 성장인자로 이루어진 군으로부터 선택된 1종 이상의 조직 성장인자를 함유하는 것을 특징으로 하는 스폰지형 제제.The method according to claim 1, wherein the tissue growth factor containing one or more tissue growth factors selected from the group consisting of platelet-derived tissue growth factor, insulin-like tissue growth factor, epithelial growth factor, fibroblast growth factor and transforming growth factor Sponge-type preparation, characterized in that. 청구항 1항 또는 청구항 3에 있어서, 상기 조직 성장인자는 키토산 스폰지에 스폰지 단위면걱당 200∼100,000ng이 함유되는 것을 특징으로 하는 스폰지형 제제.The sponge type preparation according to claim 1 or 3, wherein the tissue growth factor is contained in the chitosan sponge in a range of 200 to 100,000 ng per sponge. 청구항 4에 있어서, 상기 조직 성장인자가 미세구로 형성된 것을 특징으로 하는 스폰지형 제제.The sponge type preparation of claim 4, wherein the tissue growth factor is formed of microspheres. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019970003984A 1997-02-11 1997-02-11 Biodegradable polysaccharide sponge type formulation containing tissue growth factors for osteoblast implantation to recover KR100231279B1 (en)

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Application Number Priority Date Filing Date Title
KR1019970003984A KR100231279B1 (en) 1997-02-11 1997-02-11 Biodegradable polysaccharide sponge type formulation containing tissue growth factors for osteoblast implantation to recover

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KR100231279B1 KR100231279B1 (en) 1999-11-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100657127B1 (en) * 2004-11-29 2006-12-12 주식회사 엘지생활건강 Delivery system for tooth whitening component using in situ gelling

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030006787A (en) * 2001-07-16 2003-01-23 이승진 Chitosan bead containing tricalcium phosphate for bone substitute

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100657127B1 (en) * 2004-11-29 2006-12-12 주식회사 엘지생활건강 Delivery system for tooth whitening component using in situ gelling

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