KR970007351A - Method of Quantification of Activated Factor - Google Patents

Method of Quantification of Activated Factor Download PDF

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Publication number
KR970007351A
KR970007351A KR1019960030042A KR19960030042A KR970007351A KR 970007351 A KR970007351 A KR 970007351A KR 1019960030042 A KR1019960030042 A KR 1019960030042A KR 19960030042 A KR19960030042 A KR 19960030042A KR 970007351 A KR970007351 A KR 970007351A
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South Korea
Prior art keywords
enzyme
activated
specific
binding
immobilized
Prior art date
Application number
KR1019960030042A
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Korean (ko)
Inventor
다카오 야마모토
신고 고이즈미
위르겐 뢰미쉬
한스-아르놀트 슈퇴르
Original Assignee
미츠이 마코토
데이산 가부시키가이샤
슈타인, 라우페
베링베르케 아크티엔게젤샤프트
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Publication date
Priority claimed from JP19051295A external-priority patent/JP3447437B2/en
Application filed by 미츠이 마코토, 데이산 가부시키가이샤, 슈타인, 라우페, 베링베르케 아크티엔게젤샤프트 filed Critical 미츠이 마코토
Publication of KR970007351A publication Critical patent/KR970007351A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 예를들어, PPSB 같은 인자 농축물내(즉, 활성화 및 비활성화된 인자의 혼합물)의 단일 인자(활성화된 응집 프로테아제)의 선별을 위한 민감한 방법에 관한 것이다.The present invention relates to a sensitive method for the selection of a single factor (activated coagulation protease) in a factor concentrate such as, for example, a mixture of activated and deactivated factors, for example, PPSB.

Description

활성화된 인자의 정량방법Method of Quantification of Activated Factor

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

Claims (9)

a) 효소의 활성화된 상태에 대해 특이적인 제1결합 대응물질을 특정샘플과 함께 반응시키는 단계 및 b) 효소의 활성화된 상태에 대해 특이적인 제1결합 상대물질과 특이적으로 반응하고 직간접적으로 신호 발생 표지가 부착된 제2특이적 결합 상대물질과 효서/결합 대응물질 복합체를 반응시킴으로서 효소/결합 대응물질 복합체 형성을 탐지하는 단계를 포함하는, 상기 샘플내의 활성화된 효소를 탐지하고 결정하기 위한 이종성의 면역 화학적 방법.a) reacting a first binding counterpart specific to the activated state of the enzyme with a specific sample; and b) reacting specifically with the first binding counterpart specific to the activated state of the enzyme and directly or indirectly A method for detecting and determining an activated enzyme in a sample, said method comprising: detecting enzyme / binding-partner complex formation by reacting a second specific binding partner with a signal generation marker and a enzyme / Immunochemical methods of heterogeneity. 제1항에 있어서, 효소/결합 대응물질 복합체가 고정화되는 방법.2. The method of claim 1 wherein the enzyme / binding partner complex is immobilized. 제2항에 있어서, 고체상에 결합된 제1특이적 결합 상대물질에 의하여 고정화가 수행되는 방법.3. The method of claim 2, wherein the immobilization is performed by a first specific binding partner material bound to a solid phase. 제3항에 있어서, 고정화된 효소/결합 대응물질 복합체가, 효소성분 또는 효소/결합 대응물질 복합체에 대해 특이적이고 직간접적으로 신호 발생 표지가 부착된 항체를 사용하여 탐지되는 방법.4. The method of claim 3 wherein the immobilized enzyme / binding partner complex is detected using an antibody that is specific for the enzyme component or the enzyme / binding partner complex and that is directly or indirectly attached to the signal generating label. 제1항에 있어서, 활성화된 효소를, 고체상에 결합된 효소 특이적 항체에 의해 고정화시키고 고정화된 활성화 효소를, 효소의 활성화된 상태에 대해 특이적이고 직간접적으로 신호 발생 표지가 부착된 결합 대응물질과 반응시키는 방법.2. The method according to claim 1, wherein the activated enzyme is immobilized by an enzyme-specific antibody bound to a solid phase, and the immobilized activated enzyme is immobilized on a binding-specific substance directly or indirectly attached to the activated state of the enzyme, ≪ / RTI > 제1항 내지 제5항 중 어느 한 항에 있어서, 활성화된 효소가 활성화된 응집인자인 방법.6. The method according to any one of claims 1 to 5, wherein the activated enzyme is an activated flocculation factor. 제1항 내지 제6항 중 어느 한 항에 있어서, 신호 발생 표지가 화학 발광성 또는 형광성 표지인 방법.7. The method according to any one of claims 1 to 6, wherein the signal generation indicator is chemiluminescent or fluorescent. 제1항 내지 제7항 중 어느 한 항에 있어서, 신호 발생 표지가 효소, 바람직하게는 양고추냉이 퍼옥시다제인 방법.8. The method according to any one of claims 1 to 7, wherein the signal generation label is an enzyme, preferably horseradish peroxidase. 활성화된 인자와 비활성화된 인자의 혼합물중의 활성화된 응집인자를 결정하기 위한, 제1항 내지 제8항중 어느 한 항에 따른 방법의 용도.8. Use of a method according to any one of claims 1 to 8 for determining an activated coagulation factor in a mixture of activated and deactivated factors. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960030042A 1995-07-24 1996-07-24 Method of Quantification of Activated Factor KR970007351A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19526700.1 1995-07-24
DE19526700 1995-07-24
JP95-190512 1995-07-26
JP19051295A JP3447437B2 (en) 1995-07-26 1995-07-26 High-purity nitrogen gas production equipment

Publications (1)

Publication Number Publication Date
KR970007351A true KR970007351A (en) 1997-02-01

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995024500A1 (en) * 1994-03-10 1995-09-14 Ciba Corning Diagnostics Corp. Inhibition of protease activity of human whole blood cell lysates
WO1996003506A2 (en) * 1994-07-21 1996-02-08 Connaught Laboratories Limited Analog of haemophilus hin47 with reduced protease activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995024500A1 (en) * 1994-03-10 1995-09-14 Ciba Corning Diagnostics Corp. Inhibition of protease activity of human whole blood cell lysates
WO1996003506A2 (en) * 1994-07-21 1996-02-08 Connaught Laboratories Limited Analog of haemophilus hin47 with reduced protease activity

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