KR970002671B1 - Hidantoinase encoding dna from bacillus brevis - Google Patents

Hidantoinase encoding dna from bacillus brevis Download PDF

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Publication number
KR970002671B1
KR970002671B1 KR94004846A KR19940004846A KR970002671B1 KR 970002671 B1 KR970002671 B1 KR 970002671B1 KR 94004846 A KR94004846 A KR 94004846A KR 19940004846 A KR19940004846 A KR 19940004846A KR 970002671 B1 KR970002671 B1 KR 970002671B1
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KR
South Korea
Prior art keywords
gene
bacillus brevis
hydantoinase
protein
dna
Prior art date
Application number
KR94004846A
Other languages
Korean (ko)
Inventor
Hyung-Soon Kim
Sung-Joo Hong
Bum-Sub Noh
Mook Kim
Hong-Kyu Choe
Young-Chol Park
Yong-Joo Cho
Original Assignee
Hanwha Co Ltd
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Application filed by Hanwha Co Ltd filed Critical Hanwha Co Ltd
Priority to KR94004846A priority Critical patent/KR970002671B1/en
Application granted granted Critical
Publication of KR970002671B1 publication Critical patent/KR970002671B1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides gene encoding the hydantoinase protein separated from the Bacillus brevis having a high D-hydantoin hydrolysis activity under a high temperature and alkali condition. In order to recombine the gene, the Bacillus brevis isolate strain is cultured in a LB medium and makes a check the hydantoinase activity. The synthesized oligonucleotide based on N-terminal amino acid sequences of the protein is used in the separation of chromosome DNA from the Bacillus brevis in order to separate and clone the gene of the protein having the hydantoinase activity. The prepared O gt11 library is searched to clone the hydantoinase gene, the produced gene DNA is inserted in the Eco RI location of pUC19 of plasmid gene carrier to prepare recombinant DNA pHMY 180.
KR94004846A 1994-03-11 1994-03-11 Hidantoinase encoding dna from bacillus brevis KR970002671B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR94004846A KR970002671B1 (en) 1994-03-11 1994-03-11 Hidantoinase encoding dna from bacillus brevis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR94004846A KR970002671B1 (en) 1994-03-11 1994-03-11 Hidantoinase encoding dna from bacillus brevis

Publications (1)

Publication Number Publication Date
KR970002671B1 true KR970002671B1 (en) 1997-03-07

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ID=19378753

Family Applications (1)

Application Number Title Priority Date Filing Date
KR94004846A KR970002671B1 (en) 1994-03-11 1994-03-11 Hidantoinase encoding dna from bacillus brevis

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KR (1) KR970002671B1 (en)

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