KR960703168A - 세포흡착과 헤미데스모좀 합체를 자극하는 세포 매트릭스(cell matrix stimulated cell attachment and hemidesmosome assembly) - Google Patents

세포흡착과 헤미데스모좀 합체를 자극하는 세포 매트릭스(cell matrix stimulated cell attachment and hemidesmosome assembly) Download PDF

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KR960703168A
KR960703168A KR1019950704266A KR19950704266A KR960703168A KR 960703168 A KR960703168 A KR 960703168A KR 1019950704266 A KR1019950704266 A KR 1019950704266A KR 19950704266 A KR19950704266 A KR 19950704266A KR 960703168 A KR960703168 A KR 960703168A
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cell
cells
protein
matrix
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알. 존 조나단 씨.
비토 쿠아란타
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샤로떼 피 클락
데스모스 인코퍼레이티드
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Publication of KR960703168A publication Critical patent/KR960703168A/ko

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Abstract

본 발명은 특정 세포의 매트릭스 단백질과 이 단백질의 사용방법 그리고 이들 단백질이 피복된 모양을 갖춘 물품에 대해 상술하고 있는데 이때 모양을 갖춘 물품위에서 생장한 세포에서 세포흡착과 헤미데스모좀 형성을 자극하고, 태아췌장 섬 세포 전구체를 포함하는 매트릭스에서 생장한 엔도크린 세포 전구체들은 생물학적 기능과 분화를 유지한다.

Description

세포흡착과 헤미데스모좀 합체를 자극하는 세포 매트릭스(CELL MATRIX STIMULATED CELL ATTACHMENT AND HEMIDESMOSOME ASSEMBLY)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (34)

  1. 생체내에 포유류에 사용하기에 적합한 생체 적응성 있는 모양의 물건과 상기 모양을 갖춘 물건에 헤미데스모좀 형상을 실행하는 단백질 조성물로 구성된 물품.
  2. 제1항에 있어서, 단백질 조성물이 상피기원의 종양세포주에 의해 침전되는 것을 특징으로 하는 물품.
  3. 제2항에 있어서, 종양세포주는 쥐 암육종세포주 804G인 것을 특징으로 하는 물품.
  4. 제2항에 있어서, 종양세포주는 쥐 방광 암세포주 NBTII인 것을 특징으로 하는 물품.
  5. 제1항에 있어서, 단백질 조성물에는 세포주 804G에 의해 침전된 세포의 매트릭스의 약 100KD, 135KD, 140KD, 150KD, 400KD 단백질로 구성된 것중 하나를 포함하는 것을 특징으로 하는 물품.
  6. 제5항에 있어서, 쉬트형으로 매트릭스에 배양된 상피세포를 추가로 포함하는 것을 특징으로 하는 물품.
  7. 제1항에 있어서, 콜라겐, 재생콜라겐 또는 폴리라틱산으로 물품을 피복하는 것으로 구성된 것을 특징으로 하는 물품.
  8. 제1항에 있어서, 물품은 생체적응성 있는 물질로 만들어지거나 또는 피복되는 것을 특징으로 하는 물품.
  9. 제8항에 있어서, 금속은 스테인레스 스틸 또는 티타늄인 것을 특징으로 하는 물품.
  10. 제1항에 있어서, 물품은 세라믹 물질로 만들어지거나 피복되는 것을 특징으로 하는 물품.
  11. 제10항에 있어서, 세라믹물질로 하이드록시아파타이트인 것을 특징으로 하는 물품.
  12. 제1항에 있어서, 물품은 합성 고분자로 만들어지거나 또는 피복되는 것을 특징으로 하는 물품.
  13. 제12항에 있어서, 고분자는 폴리에스테르 또는 나일론인 것을 특징으로 하는 물품.
  14. 포유류 세포를 성장시키는데 사용되는 조성물의 구성에 있어서 : 포유류 세포의 매트릭스 단백질; 이때 단백질은 이 단백질에 세포가 접촉하는 경우에 헤미데스모좀 형성을 촉진시키는 능력을 가지고; 그리고 제약학적 수용가능한 담체로 구성된 것을 특징으로 하는 물품.
  15. 실제 분리된 형태로 세포주 804G에 의해 침전되는 하나이상의 단백질성 세포의 매트릭스 단백질.
  16. SEQ IN NO : 1를 포함하고 배양된 상피세포에 헤미데스모좀 형성을 실행시킬 수 있는 능력을 가지는 150KD 단백질을 기본 구성으로 하는 분리된 형의 폴리펩티드.
  17. 동종이식편 이용을 위한 피부를 만드는 방법에 잇어서 : 제1항에 따른 물품 위에 상피세포를 배양하고; 그리고 피부 성장 촉진조건하에 세포를 배양시키는 것으로 구성된 것을 특징으로 하는 방법.
  18. 표적표면에 상피세포 흡착을 증가시키는 방법에 있어서 : 세포주 804G에 의해 침전되는 약 100KD, 135KD, 140KD, 150KD, 400KD 단백질중 하나로 표면을 복하는 것을 특징으로 하는 방법.
  19. 제18항에 있어서, 세포는 치근막 세포인 것을 특징으로 하는 방법.
  20. 제18항에 있어서, 세포흡착은 생체외에서 일어하는 것을 특징으로 하는 방법.
  21. 제18항에 있어서, 세포흡착은 생체내외서 일어하는 것을 특징으로 하는 방법.
  22. 엔도크린 전구체 세포를 생장시키는 방법에 있어서, 세포의 강화된 성장을 촉진시킬 수 있는 804G 매트릭스 단백질 존재하에 엔도크린 전구체 세포를 배양하는 단계로 구성된 것을 특징으로 하는 방법.
  23. 제22항에 있어서, 엔드크린 전구체 세포는 췌장 섬 세포 전구체인 것을 특징으로 하는 방법.
  24. 제23항에 있어서, 배양단계전에 : 태아 췌장을 효소적으로 절단하고; 섬형 세포 응집체가 형성될때까지 세포배양 배지에서 절단된 웨장을 배양하는 단계가 추가로 구성된 것을 특징으로 하는 방법.
  25. 제23항에 있어서, 췌장은 포유류에서 취한 것을 특징으로 하는 방법.
  26. 제23항에 있어서, 췌장은 사람에서 취한 것을 특징으로 하는 방법.
  27. 제22항에 있어서, 804G 매트릭스 단백질은 기질에 부착된 고형상 단백질인 것을 특징으로 하는 방법.
  28. 제22항에 있어서, 매트릭스는 804G 쥐방광 암육종 세포에서 유도한 것을 특징으로 하는 방법.
  29. 호르몬 생산세포의 발생방법에 있어서 : 제1항의 방법에 따라 확장된 엔도크린 전구체 세포를 제공하고 ; 포유류내로 확장된 엔도크린 전구체 세포를 이식하는 단계로 구성된 것을 특징으로 하는 방법.
  30. 제28항에 있어서, 세포는 췌장 섬세포인 것을 특징으로 하는 방법.
  31. 제28항에 있어서, 호르몬은 인술인 것을 특징으로 하는 방법.
  32. 제31항에 있어서, 세포는 신장, 폐 또는 간에 또는 인접하여 이식되는 것을 특징으로 하는 방법.
  33. 제22항에 따라 준비된 것을 특징으로 하는 전구세포.
  34. 제33항에 있어서, 태아췌장 섬 전구세포인 것을 특징으로 하는 전구세포.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950704266A 1993-04-05 1994-04-05 세포흡착과 헤미데스모좀 합체를 자극하는 세포 매트릭스(cell matrix stimulated cell attachment and hemidesmosome assembly) KR960703168A (ko)

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US4272793A 1993-04-05 1993-04-05
US08/042,727 1993-04-05
US08/152,460 US5510263A (en) 1993-04-05 1993-11-12 Growth of pancreatic islet-like cell clusters
US08/152,460 1993-11-12
PCT/US1994/003733 WO1994023016A1 (en) 1993-04-05 1994-04-05 Cell matrix stimulated cell attachment and hemidesmosome assembly

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SG44568A1 (en) 1997-12-19
CA2159471A1 (en) 1994-10-13
US5510263A (en) 1996-04-23
CN1124978A (zh) 1996-06-19
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AU686617B2 (en) 1998-02-12
AU6555794A (en) 1994-10-24

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