KR960037840A - Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. - Google Patents

Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. Download PDF

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Publication number
KR960037840A
KR960037840A KR1019950010781A KR19950010781A KR960037840A KR 960037840 A KR960037840 A KR 960037840A KR 1019950010781 A KR1019950010781 A KR 1019950010781A KR 19950010781 A KR19950010781 A KR 19950010781A KR 960037840 A KR960037840 A KR 960037840A
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South Korea
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algae
toxicity
toxic substances
contact
present
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KR1019950010781A
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Korean (ko)
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이상일
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이상일
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Priority to KR1019950010781A priority Critical patent/KR960037840A/en
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Abstract

본 발명은 상수 및 폐수중 독성물질(유기독성, 중금속, CN류 및 농약류)이나 유해폐기물의 독성도를 측정하는 방법을써 보다 신속하고, 경제적이며 민감도를 높일 수 있는 Daphnia magna 및 Ceriodaphnia dubia 등 물벼룩을 이용한 독성조사방법에 관한 것이다.The present invention is a method for measuring the toxicity of toxic substances (organic toxicity, heavy metals, CNs and pesticides) or hazardous wastes in water and wastewater by using a method such as Daphnia magna and Ceriodaphnia dubia, which can increase the speed, economy and sensitivity more quickly. Toxicity investigation method used.

Daphnia magna 및 Ceriodaphnia dubia 등 물벼룩의 조류(algae) 섭생기작에 근거한 급성독성조사방법으로써 독성실험전 어린 물벼룩의 내장을 투명하게 유지하기 위해 효모만을 먹이로 공급하거나 먹이를 주입치 않고 배양한 후 독성물질에 접촉시키고 먹이로 조류(algae)를 공급하여 조류 섭생여부를 판별하여 단시간내 독성여부를 정밀하고 민감하게 측정하는 방법을 발명하였다. 본 발명의 이용가능성을 검토하기 위한 실험예에서는 내장내 녹조류가 없는 물벼룩의 유생을 독성물질들에 1∼6시간 접촉시킨 다음 녹조류인 Scenedasmus sp.를 먹이로 공급하여 섭생하게 한 후 광학 및 형광현미경으로 섭생여부를 관찰하였다. 녹조류의 섭생은 정상 상태로 간주하고 녹조류를 섭생치 않은 것은 독성물질의 피해를 입은 비정상 상태로 간주하였다. 1∼6시간의 독성물질(중금속류, 시안류, pentachorophenol 및 농약류) 접촉에 따른 접촉시간의 영향은 크지 않았으며, 1시간 접촉시킨 후 녹조류 섭생을 이용한 급성독성 조사법과 널리 이용되고 있는 표준 48시간 조사법을 비교한 결과 본 발명의 방법인 1시간 급성독성조사법이 민감도에서 차이가 크지 않아 새로운 독성조사법으로 타당함이 판명되었다. 본 발명은 기존의 독성조사방법에서의 단점으로 지적된 고가의 장비사용이나, 측정방법의 전문성, 방법에 따른 민감한 독성물질 차이와 조사에 걸리는 시간등을 보완할 수 있는 것으로 판명되었다.It is an acute toxicity test based on algae regime of water fleas such as Daphnia magna and Ceriodaphnia dubia. It is a toxic substance after feeding only yeast or cultivating it without feeding to keep the viscera of young fleas transparent. It was invented a method for precisely and sensitively measuring the toxicity in a short time by determining whether the algae is fed by contacting and feeding algae as food. In the experimental example for examining the applicability of the present invention, the larvae of intestinal green algae without contact with toxic substances were contacted with toxic substances for 1 to 6 hours, and then fed to feed algae Scenedasmus sp. It was observed whether the regimen. The green algae regime was considered normal and the nonalgae algae were considered to be abnormal with damage from toxic substances. The effect of contact time on contact with toxic substances (heavy metals, cyanide, pentachorophenol and pesticides) for 1 to 6 hours was not significant. After 1 hour contact, acute toxicity test using green algae regime and standard 48 hour method widely used As a result, it was found that the 1 hour acute toxicity test method of the present invention was not significantly different in sensitivity, and thus it was justified as a new toxicity test method. The present invention was found to be able to compensate for the use of expensive equipment pointed out as a disadvantage in the existing toxicity investigation method, the expertise of the measurement method, the difference of sensitive toxic substances according to the method and the time taken for the investigation.

Description

물벼룩(Daphnia sp. 및 Ceriodaphnia sp.)의 조류(algae)섭생에 근거한 급성조사방법Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (5)

유해독성물질(중금속, 유기독성, 농약류등)의 독성률 측정방법중 물벼룩(예, Daphnia sp. 및 Ceriodaphnia sp. 등)의 섭생기작(feeding behavior)을 이용하는 측정방법.Measurement method using the feeding behavior of water flea (eg Daphnia sp. And Ceriodaphnia sp.) Among the methods of measuring the toxicity rate of harmful toxic substances (heavy metal, organic toxicity, pesticides, etc.). 형광물질로 피막한 조류(algae)를 먹이로 이용하는 방법.Algae coated with a fluorescent material is used as a food. 먹이로 조류배양물을 직접 이용하는 방법.How to use bird culture directly as food. 물벼룩의 섭생을 이용한 독성측정방법중 단시간(6시간 이하) 측정방법.Method of measuring short time (less than 6 hours) among the toxicity measurement method using water flea regimen. 위의 1항, 2항, 3항을 이용한 상수 및 폐수내의 독성을 연속모니터링하는 방법.Method for continuous monitoring of toxicity in wastewater and constants using paragraphs 1, 2, and 3 above. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950010781A 1995-04-29 1995-04-29 Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. KR960037840A (en)

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KR1019950010781A KR960037840A (en) 1995-04-29 1995-04-29 Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp.

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KR1019950010781A KR960037840A (en) 1995-04-29 1995-04-29 Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp.

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100407753B1 (en) * 2000-12-29 2003-12-01 이찬원 Method of culturing domestic daphnia for environmental toxicity evaluation and method of measuring toxicity by use of same
KR100426791B1 (en) * 2001-12-22 2004-04-14 학교법인조선대학교 A method for ecological treatment of organic wastewater using alga and waterflea
WO2016133230A1 (en) * 2015-02-16 2016-08-25 동국대학교 산학협력단 Biomarker for determining exposure to chrome and determining method using same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100407753B1 (en) * 2000-12-29 2003-12-01 이찬원 Method of culturing domestic daphnia for environmental toxicity evaluation and method of measuring toxicity by use of same
KR100426791B1 (en) * 2001-12-22 2004-04-14 학교법인조선대학교 A method for ecological treatment of organic wastewater using alga and waterflea
WO2016133230A1 (en) * 2015-02-16 2016-08-25 동국대학교 산학협력단 Biomarker for determining exposure to chrome and determining method using same

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