KR960037840A - Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. - Google Patents
Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. Download PDFInfo
- Publication number
- KR960037840A KR960037840A KR1019950010781A KR19950010781A KR960037840A KR 960037840 A KR960037840 A KR 960037840A KR 1019950010781 A KR1019950010781 A KR 1019950010781A KR 19950010781 A KR19950010781 A KR 19950010781A KR 960037840 A KR960037840 A KR 960037840A
- Authority
- KR
- South Korea
- Prior art keywords
- algae
- toxicity
- toxic substances
- contact
- present
- Prior art date
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 상수 및 폐수중 독성물질(유기독성, 중금속, CN류 및 농약류)이나 유해폐기물의 독성도를 측정하는 방법을써 보다 신속하고, 경제적이며 민감도를 높일 수 있는 Daphnia magna 및 Ceriodaphnia dubia 등 물벼룩을 이용한 독성조사방법에 관한 것이다.The present invention is a method for measuring the toxicity of toxic substances (organic toxicity, heavy metals, CNs and pesticides) or hazardous wastes in water and wastewater by using a method such as Daphnia magna and Ceriodaphnia dubia, which can increase the speed, economy and sensitivity more quickly. Toxicity investigation method used.
Daphnia magna 및 Ceriodaphnia dubia 등 물벼룩의 조류(algae) 섭생기작에 근거한 급성독성조사방법으로써 독성실험전 어린 물벼룩의 내장을 투명하게 유지하기 위해 효모만을 먹이로 공급하거나 먹이를 주입치 않고 배양한 후 독성물질에 접촉시키고 먹이로 조류(algae)를 공급하여 조류 섭생여부를 판별하여 단시간내 독성여부를 정밀하고 민감하게 측정하는 방법을 발명하였다. 본 발명의 이용가능성을 검토하기 위한 실험예에서는 내장내 녹조류가 없는 물벼룩의 유생을 독성물질들에 1∼6시간 접촉시킨 다음 녹조류인 Scenedasmus sp.를 먹이로 공급하여 섭생하게 한 후 광학 및 형광현미경으로 섭생여부를 관찰하였다. 녹조류의 섭생은 정상 상태로 간주하고 녹조류를 섭생치 않은 것은 독성물질의 피해를 입은 비정상 상태로 간주하였다. 1∼6시간의 독성물질(중금속류, 시안류, pentachorophenol 및 농약류) 접촉에 따른 접촉시간의 영향은 크지 않았으며, 1시간 접촉시킨 후 녹조류 섭생을 이용한 급성독성 조사법과 널리 이용되고 있는 표준 48시간 조사법을 비교한 결과 본 발명의 방법인 1시간 급성독성조사법이 민감도에서 차이가 크지 않아 새로운 독성조사법으로 타당함이 판명되었다. 본 발명은 기존의 독성조사방법에서의 단점으로 지적된 고가의 장비사용이나, 측정방법의 전문성, 방법에 따른 민감한 독성물질 차이와 조사에 걸리는 시간등을 보완할 수 있는 것으로 판명되었다.It is an acute toxicity test based on algae regime of water fleas such as Daphnia magna and Ceriodaphnia dubia. It is a toxic substance after feeding only yeast or cultivating it without feeding to keep the viscera of young fleas transparent. It was invented a method for precisely and sensitively measuring the toxicity in a short time by determining whether the algae is fed by contacting and feeding algae as food. In the experimental example for examining the applicability of the present invention, the larvae of intestinal green algae without contact with toxic substances were contacted with toxic substances for 1 to 6 hours, and then fed to feed algae Scenedasmus sp. It was observed whether the regimen. The green algae regime was considered normal and the nonalgae algae were considered to be abnormal with damage from toxic substances. The effect of contact time on contact with toxic substances (heavy metals, cyanide, pentachorophenol and pesticides) for 1 to 6 hours was not significant. After 1 hour contact, acute toxicity test using green algae regime and standard 48 hour method widely used As a result, it was found that the 1 hour acute toxicity test method of the present invention was not significantly different in sensitivity, and thus it was justified as a new toxicity test method. The present invention was found to be able to compensate for the use of expensive equipment pointed out as a disadvantage in the existing toxicity investigation method, the expertise of the measurement method, the difference of sensitive toxic substances according to the method and the time taken for the investigation.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950010781A KR960037840A (en) | 1995-04-29 | 1995-04-29 | Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950010781A KR960037840A (en) | 1995-04-29 | 1995-04-29 | Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. |
Publications (1)
Publication Number | Publication Date |
---|---|
KR960037840A true KR960037840A (en) | 1996-11-19 |
Family
ID=66523719
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019950010781A KR960037840A (en) | 1995-04-29 | 1995-04-29 | Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR960037840A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100407753B1 (en) * | 2000-12-29 | 2003-12-01 | 이찬원 | Method of culturing domestic daphnia for environmental toxicity evaluation and method of measuring toxicity by use of same |
KR100426791B1 (en) * | 2001-12-22 | 2004-04-14 | 학교법인조선대학교 | A method for ecological treatment of organic wastewater using alga and waterflea |
WO2016133230A1 (en) * | 2015-02-16 | 2016-08-25 | 동국대학교 산학협력단 | Biomarker for determining exposure to chrome and determining method using same |
-
1995
- 1995-04-29 KR KR1019950010781A patent/KR960037840A/en not_active Application Discontinuation
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100407753B1 (en) * | 2000-12-29 | 2003-12-01 | 이찬원 | Method of culturing domestic daphnia for environmental toxicity evaluation and method of measuring toxicity by use of same |
KR100426791B1 (en) * | 2001-12-22 | 2004-04-14 | 학교법인조선대학교 | A method for ecological treatment of organic wastewater using alga and waterflea |
WO2016133230A1 (en) * | 2015-02-16 | 2016-08-25 | 동국대학교 산학협력단 | Biomarker for determining exposure to chrome and determining method using same |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Kaiser | Correlations of Vibrio fischeri bacteria test data with bioassay data for other organisms. | |
Kholodkevich et al. | Applicability of a bioelectronic cardiac monitoring system for the detection of biological effects of pollution in bioindicator species in the Gulf of Finland | |
Asif et al. | A review of on environmental pollution bioindicators | |
Kahru et al. | Biotests and biosensors in ecotoxicological risk assessment of field soils polluted with zinc, lead, and cadmium | |
Lee et al. | Declining mercury concentrations in bluefin tuna reflect reduced emissions to the North Atlantic Ocean | |
Kanaya et al. | Impacts of the 2011 tsunami on sediment characteristics and macrozoobenthic assemblages in a shallow eutrophic lagoon, Sendai Bay, Japan | |
Pinsino et al. | Sea urchin coelomocytes as a novel cellular biosensor of environmental stress: a field study in the Tremiti Island Marine Protected Area, Southern Adriatic Sea, Italy | |
Renedo et al. | Contrasting spatial and seasonal trends of methylmercury exposure pathways of Arctic seabirds: combination of large-scale tracking and stable isotopic approaches | |
Copp | Is fish condition correlated with water conductivity? | |
Hussain et al. | Variation in genotoxic susceptibility and biomarker responses in Cirrhinus mrigala and Catla catla from different ecological niches of the Chenab River | |
Pastor et al. | A 4 year follow‐up analysis of genotoxic damage in birds of the Doñana area (south west Spain) in the wake of the 1998 mining waste spill | |
Salazar et al. | Using caged bivalves to characterize exposure and effects associated with pulp and paper mill effluents | |
Erismis et al. | Evaluation of DNA damage in Eurasian Marsh Frogs (Pelophylax ridibundus) by Comet Assay for determination of possible pollution in the different lakes in Central Anatolia, Turkey | |
Agell et al. | Does stress protein induction by copper modify natural toxicity in sponges? | |
Ernst | Effects of pesticides and related organic compounds in the sea | |
Erraud et al. | Use of sperm DNA integrity as a marker for exposure to contamination in Palaemon serratus (Pennant 1777): Intrinsic variability, baseline level and in situ deployment | |
KR960037840A (en) | Acute Investigation Method Based on Algae Regeneration of Daphnia sp. And Ceriodaphnia sp. | |
Vetemaa | Reproduction parameters of fish as indicators in environmental monitoring | |
Antipa | Use of commensal protozoa as biological indicators of water quality and pollution | |
Stone et al. | Pulse‐Exposure Toxicity of Ammonia and Propoxur to the Tropical Copepod Acartia sinjiensis | |
Pearce et al. | A review of monitoring strategies and assessments of estuarine pollution | |
Yomogida et al. | Higher risk of fatality by predatory attacks in earlier ontogenetic stages of modern Nautilus pompilius in the Philippines: evidence from the ontogenetic analyses of shell repairs | |
ATE249623T1 (en) | METHOD FOR MONITORING NYLON 11 PARTS | |
Lockett | The effect of rotenone on fishes and its use as a sampling technique: a survey | |
Dieter et al. | Evaluation of the Microtox test to detect phorate contamination in wetlands |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E601 | Decision to refuse application |