KR960035021A - Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 - Google Patents
Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 Download PDFInfo
- Publication number
- KR960035021A KR960035021A KR1019950004261A KR19950004261A KR960035021A KR 960035021 A KR960035021 A KR 960035021A KR 1019950004261 A KR1019950004261 A KR 1019950004261A KR 19950004261 A KR19950004261 A KR 19950004261A KR 960035021 A KR960035021 A KR 960035021A
- Authority
- KR
- South Korea
- Prior art keywords
- epitope
- hepatitis
- surface antigen
- pres2
- protein
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 B형 간염 표면항원 preS2의 에피토프 태깅을 이용한 단백질의 면역화학적 분석 방법에 관한 것으로, 단백질을 코딩하는 뉴클레오티드 서열의 5′또는 3′말단에 B형 간염 표면 항원 preS2의 에피토프를 코딩하는 뉴클레오티드 서열을 삽입하여 에피토프로 태깅된 융합 단백질을 생산하고, 모노클로날 항체 H8을 이용하여 연구대상 단백질을 면역화학적으로 감지 및 분리하는 방법이다. 이 방법에 의해 연구 대상 단백질의 특정 항체가 없어도 이를 쉽게 감지 및 분리할 수 있다.The present invention relates to a method for immunochemical analysis of proteins using epitope tagging of hepatitis B surface antigen preS2, wherein the nucleotide encoding the epitope of hepatitis B surface antigen preS2 at the 5 'or 3' end of the nucleotide sequence encoding the protein. By inserting the sequence, epitope-tagged fusion proteins are produced, and the monoclonal antibody H8 is used to immunochemically detect and isolate the protein of interest. This method makes it easy to detect and isolate specific antibodies in the protein under study.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 preS2-β-갈락토시다제 융합 단백질을 코딩하는 플라스미드(제1A도)와 preS2-에피토프 카세트(제1B도)를 개략적으로 나타낸 것이고, 제2도는 preS2 에피토프로 태깅된 β-갈락토시다제를 모노 클로날 항제 H8을 이용하여 분석한 결과이고, 제3도는 preS2-p56lck융합 단백질을 코딩하는 플라스미드(제3A도)와 preS2-에피토프 카세트(제3B도)를 개략적으로 나타낸 것이고, 제4도는 모노클로날 항체 H8을 이용하여 preS2로 태깅된 p56lck단편을 면역 침전한 결과를 나타내는 것이다.FIG. 1 schematically shows the plasmid encoding the preS2-β-galactosidase fusion protein (FIG. 1A) and the preS2- epitope cassette (FIG. 1B), and FIG. 2 shows the β-galacto tagged with the preS2 epitope. As a result of analysis of the sidase using the monoclonal agent H8, FIG. 3 schematically shows the plasmid encoding the preS2-p56 lck fusion protein (Figure 3A) and the preS2- epitope cassette (Figure 3B), 4 shows the result of immunoprecipitation of the p56 lck fragment tagged with preS2 using monoclonal antibody H8.
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950004261A KR960035021A (en) | 1995-03-02 | 1995-03-02 | Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950004261A KR960035021A (en) | 1995-03-02 | 1995-03-02 | Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR960035021A true KR960035021A (en) | 1996-10-24 |
Family
ID=66549281
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019950004261A KR960035021A (en) | 1995-03-02 | 1995-03-02 | Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR960035021A (en) |
-
1995
- 1995-03-02 KR KR1019950004261A patent/KR960035021A/en not_active Application Discontinuation
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Capra et al. | Light chain sequences of human IgM cold agglutinins | |
ATE468127T1 (en) | II-KEY/ANTIGENIC EPITOP HYBRID PEPTIDE VACCINE | |
DK532284A (en) | PROCEDURE FOR DETERMINING ANTI-ANTI-ACTIVE AMINO ACID SEQUENCES | |
NO975435L (en) | Modified / chimeric proteins and their use | |
BR9809443A (en) | Polypeptide comprising an antigenic portion of an antigen of m. tuberculosis, dna molecule, recombinant expression vector, host cell, process for detection of m. tuberculosis in a biological sample, diagnostic kit, monoclonal antibody, polyclonal antibody, and fusion protein. | |
GEP20012421B (en) | Method for Typing Hepatitis C Virus and Reagents for Use Therein | |
DE69111801D1 (en) | VACCINE AGAINST HEPATITIS B. | |
DE58905081D1 (en) | METHOD FOR DETERMINING A PROTEIN ON THE PRINCIPLE OF THE FLUORESCENT POLARIZATION IMMUNOASSAY. | |
DK0644202T3 (en) | Synthetic antigens for detection of antibodies against hepatitis C virus | |
ES2129487T3 (en) | SYNTHETIC PEPTIDES, ANTIBODIES DIRECTED AGAINST THEM AND THEIR USE. | |
EP0696640A3 (en) | Recombinant antigen from the NS3 region of Hepatitis C virus | |
KR950008537A (en) | Diagnostic Agents for Detection of Antibodies Against Epstein Bacillus (EBV) | |
JPS6470498A (en) | Synthetic peptide and antibody thereto | |
KR930008155A (en) | Monoclonal Antibodies Against Hepatitis Hepatitis Virus (HCV) | |
Natsume et al. | Rapid analysis of protein interactions: On‐chip micropurification of recombinant protein expressed in Esherichia coli | |
ES2113356T3 (en) | PEPTIDES WITH AN CHARACTERISTIC ANTIGENIC DETERMINANT FOR ALPHA-1-MICRO-GLOBULIN. | |
ATE211172T1 (en) | EPSTEIN-BARR VIRUS PEPTIDES, AND ANTIBODIES AGAINST THESE PEPTIDES | |
EP0582243A3 (en) | HCV peptide antigens and a method of testing for the hepatitis C virus (HCV). | |
KR960035021A (en) | Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 | |
Park et al. | Targeted destruction of the polymerized human serum albumin binding site within the preS2 region of the HBV surface antigen while retaining full immunogenicity for this epitope | |
DE69836647D1 (en) | METHOD FOR THE PRODUCTION OF (POLY) PEPTIDES USING CONCRETE VARIANTS OF SV40 'LARGE' T ANTIGEN WITH AN INTACT N TERMINUS | |
KR930004328A (en) | Non-A, Non-B Type Peptides | |
NO910479L (en) | SYNTHETIC POLYPEPTIDES AND ANTIBODIES RELATED TO EPSTEIN-BARR VIRUS CORE ANTIGEN. | |
KR930007970A (en) | Non-A, Non-Type Peptides | |
BR9105983A (en) | MONOCLONAL ANTIBODIES THAT DIFFERENTIATE BETWEEN NATIVE AND MODIFIED SEQUENCE PROTEINS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E601 | Decision to refuse application |