KR960035021A - Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 - Google Patents

Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 Download PDF

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Publication number
KR960035021A
KR960035021A KR1019950004261A KR19950004261A KR960035021A KR 960035021 A KR960035021 A KR 960035021A KR 1019950004261 A KR1019950004261 A KR 1019950004261A KR 19950004261 A KR19950004261 A KR 19950004261A KR 960035021 A KR960035021 A KR 960035021A
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KR
South Korea
Prior art keywords
epitope
hepatitis
surface antigen
pres2
protein
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KR1019950004261A
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Korean (ko)
Inventor
유명희
강현아
이관수
권기선
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김은영
한국과학기술연구원
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Priority to KR1019950004261A priority Critical patent/KR960035021A/en
Publication of KR960035021A publication Critical patent/KR960035021A/en

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Abstract

본 발명은 B형 간염 표면항원 preS2의 에피토프 태깅을 이용한 단백질의 면역화학적 분석 방법에 관한 것으로, 단백질을 코딩하는 뉴클레오티드 서열의 5′또는 3′말단에 B형 간염 표면 항원 preS2의 에피토프를 코딩하는 뉴클레오티드 서열을 삽입하여 에피토프로 태깅된 융합 단백질을 생산하고, 모노클로날 항체 H8을 이용하여 연구대상 단백질을 면역화학적으로 감지 및 분리하는 방법이다. 이 방법에 의해 연구 대상 단백질의 특정 항체가 없어도 이를 쉽게 감지 및 분리할 수 있다.The present invention relates to a method for immunochemical analysis of proteins using epitope tagging of hepatitis B surface antigen preS2, wherein the nucleotide encoding the epitope of hepatitis B surface antigen preS2 at the 5 'or 3' end of the nucleotide sequence encoding the protein. By inserting the sequence, epitope-tagged fusion proteins are produced, and the monoclonal antibody H8 is used to immunochemically detect and isolate the protein of interest. This method makes it easy to detect and isolate specific antibodies in the protein under study.

Description

B형 간염 표면항원 preS2의 에피토프 태깅을 이용한 단백질의 면역화학적 분석 방법Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 preS2－β－갈락토시다제 융합 단백질을 코딩하는 플라스미드(제1A도)와 preS2－에피토프 카세트(제1B도)를 개략적으로 나타낸 것이고, 제2도는 preS2 에피토프로 태깅된 β－갈락토시다제를 모노 클로날 항제 H8을 이용하여 분석한 결과이고, 제3도는 preS2－p56lck융합 단백질을 코딩하는 플라스미드(제3A도)와 preS2－에피토프 카세트(제3B도)를 개략적으로 나타낸 것이고, 제4도는 모노클로날 항체 H8을 이용하여 preS2로 태깅된 p56lck단편을 면역 침전한 결과를 나타내는 것이다.FIG. 1 schematically shows the plasmid encoding the preS2-β-galactosidase fusion protein (FIG. 1A) and the preS2- epitope cassette (FIG. 1B), and FIG. 2 shows the β-galacto tagged with the preS2 epitope. As a result of analysis of the sidase using the monoclonal agent H8, FIG. 3 schematically shows the plasmid encoding the preS2-p56 lck fusion protein (Figure 3A) and the preS2- epitope cassette (Figure 3B), 4 shows the result of immunoprecipitation of the p56 lck fragment tagged with preS2 using monoclonal antibody H8.

Claims (3)

단백질을 코딩하는 뉴클레오티드 서열의 5′또는 3′말단에 B형 간염 표면 항원 preS2의 에피토프를 코딩하는 뉴클레오티드 서열을 삽입하여 에피토프로 태깅된 융합 단백질을 생산하고, 상기 융합 단백질을 preS2의 모노클로날 항체 H8과 반응시키는 것을 포함하는, 단백질의 면역화학적 분석 방법.A nucleotide sequence encoding an epitope of hepatitis B surface antigen preS2 is inserted at the 5 'or 3' end of the nucleotide sequence encoding the protein to produce a fusion protein tagged with the epitope, and the fusion protein is a monoclonal antibody of preS2. A method of immunochemical analysis of a protein comprising reacting with H8. 제1항에 있어서, 상기 B형 간염 표면항원 preS2의 에피토프가 적어도 하기 아미노산 서열을 갖는 것을 특징으로 하는 방법:The method of claim 1, wherein the epitope of hepatitis B surface antigen preS2 has at least the following amino acid sequence: Ala Leu Xaa Asp Pro Arg Val Arg Gly Leu Tyr Phe ProAla Leu Xaa Asp Pro Arg Val Arg Gly Leu Tyr Phe Pro (여기서, Xaa는 20종의 아미노산중 어느 하나이다).Wherein Xaa is any one of 20 amino acids. 제2항에 있어서, 상기 에피토프가 하기 아미노산 서열을 갖는 것을 특징으로 하는 방법:The method of claim 2, wherein the epitope has the following amino acid sequence: Ala Leu Xaa Asp Pro Arg Val Arg Gly Leu Tyr Phe Pro AlaAla Leu Xaa Asp Pro Arg Val Arg Gly Leu Tyr Phe Pro Ala (여기서, Xaa는 20종의 아미노산중 어느 하나이다).Wherein Xaa is any one of 20 amino acids. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950004261A 1995-03-02 1995-03-02 Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2 KR960035021A (en)

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KR1019950004261A KR960035021A (en) 1995-03-02 1995-03-02 Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2

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KR1019950004261A KR960035021A (en) 1995-03-02 1995-03-02 Immunochemical Analysis of Proteins Using Epitope Tagging of Hepatitis B Surface Antigen preS2

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