KR960034427A - Method for preparing high purity sugar transition stevioside - Google Patents

Method for preparing high purity sugar transition stevioside Download PDF

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Publication number
KR960034427A
KR960034427A KR1019950004524A KR19950004524A KR960034427A KR 960034427 A KR960034427 A KR 960034427A KR 1019950004524 A KR1019950004524 A KR 1019950004524A KR 19950004524 A KR19950004524 A KR 19950004524A KR 960034427 A KR960034427 A KR 960034427A
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KR
South Korea
Prior art keywords
stevioside
yeast
sugar
high purity
producing
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KR1019950004524A
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Korean (ko)
Inventor
김정렬
육철
조석철
Original Assignee
이영길
두산종합식품 주식회사
백운화
두산기술원연구조합
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Application filed by 이영길, 두산종합식품 주식회사, 백운화, 두산기술원연구조합 filed Critical 이영길
Priority to KR1019950004524A priority Critical patent/KR960034427A/en
Publication of KR960034427A publication Critical patent/KR960034427A/en

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Abstract

본 발명은 고순도 스테비오사이드의 제조방법에 관한 것으로, 좀 더 구체적으로 당전이 스테비오사이드에 탄수화물 가수분해 효소 또는 전분질 가수분해 효소를 첨가시켜 상기 당전이 스테비오사이드에 잔존하고 있는 말토덱스트린 및 올리고당을 포도당 및 말토스 단위로 분해한 다음, 효모를 첨가시켜 2~7일 배양한 후, 발효된 균주를 제거시켜 고순도 당전이 스테비오사이드를 제조하는 방법에 관한 것이다.The present invention relates to a method for producing high-purity stevioside, and more specifically, to sugar stevioside by adding a carbohydrate hydrolase or starch hydrolase, the sugar metabolism remaining in stevioside glucose and maltodextrin and oligosaccharides After decomposing into maltose unit, and then incubated for 2-7 days by adding yeast, and to remove the fermented strain relates to a method for producing a high purity sugar transition stevioside.

Description

고순도 당전이 스테비오사이드의 제조방법Method for preparing high purity sugar transition stevioside

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (8)

당전이 스테비오사이드에 탄수화물 가수분해 효소 또는 전분질 가수분해 효소를 첨가시켜 상기 당전이 스테비오사이드에 잔존하고 있는 말토덱스트린을 포도당 및 올리고당 및 말토스 단위로 분해한 다음, 효모를 첨가시켜 배양한 후, 발효된 균주를 제거시키는 것을 특징으로 하는 고순도 당전이 스테비오사이드의 제조방법.Carbohydrate hydrolase or starch hydrolase is added to sugar stevioside to decompose maltodextrin remaining in stevioside into glucose, oligosaccharide and maltose units, and then cultured by adding yeast, followed by fermentation. Method for producing a high purity sugar stevioside, characterized in that to remove the strain. 제1항에 있어서, 상기 탄수화물 가수분해 효소 또는 전분질 가수분해 효소가 α-글루코아밀라제, 셀롤라제, β-아밀라제, β-그루코시다제, α-글루코시다제 및 α-아밀라제로 구성된 군으로부터 하나 또는 그 이상 선택됨을 특징으로 하는 고순도 당전이 스테비오 사이드의 제조방법.The method of claim 1, wherein the carbohydrate or starch hydrolase is one from the group consisting of α-glucoamylase, cellulase, β-amylase, β-glucosidase, α-glucosidase and α-amylase. Or higher select sugar stevioside, characterized in that the selected method. 제1항 또는 제2항에 있어서, 상기 효소의 양이 당전이 스테비오사이드를 고형분 100중량부에 대하여 0.001~2중량부이고, 반응온도는 40~70℃이며, pH 3.5~7.5이고, 고형분 농도 20% 이상에서 2~40시간 반응시키는 것을 특징으로 하는 고순도 당전이 스테비오사이드의 제조방법.The method according to claim 1 or 2, wherein the amount of the enzyme is 0.001 to 2 parts by weight based on 100 parts by weight of sugar stevioside, the reaction temperature is 40 ~ 70 ℃, pH 3.5 ~ 7.5, solid content concentration Method for producing a high purity sugar transition stevioside, which is reacted at 20% or more for 2 to 40 hours. 제1항에 있어서, 상기 효모가 빵효모 또는 맥주효모임을 특징으로 하는 고순도 당전이 스테비오사이드의 제조방법.The method of claim 1, wherein the yeast is baker's yeast or brewer's yeast. 제1항에 있어서, 상기 효모의 첨가방법이 직접 첨가, 고정화 효모 첨가 또는 고정화효모로 구성된 칼럼을 이용하는 것을 특징으로 하는 고순도 당전이 스테비오사이드의 제조방법.The method for producing high purity sugar transition stevioside according to claim 1, wherein the yeast addition method uses a column consisting of direct addition, immobilized yeast addition, or immobilized yeast. 제1항에 있어서, 상기 발효시 당전이 스테비오사이드의 농도가 10 내지 40%에서 2~7일 배양시킴을 특징으로 하는 고순도 당전이 스테비오사이드의 제조방법.The method of claim 1, wherein the fermentation of steviosides is incubated for 2 to 7 days at a concentration of 10 to 40% during fermentation. 제5항에 있어서, 상기 효모의 직접 첨가시 당전이 스테비오사이드 고형분 100g에 대하여 효모를 0.5~10g으로 고정화 효모의 경우에는 전체부피로 5~20㎖로 첨가시키는 것을 특징으로 하는 고순도 당전이 스테비오사이드의 제조방법.The high-purity sugar sugar stevioside according to claim 5, wherein when the yeast is directly added to the stevioside solid content of 100 g, the yeast immobilized at 0.5 to 10 g is added at a total volume of 5 to 20 ml. Manufacturing method. 제1항 내지 제7항 중 어느 한 항의 방법으로 제조된 고순도 당전이 스테비오사이드를 함유하는 음료, 주류, 과자, 장류, 절임식품 또는 다이어트 식품.A beverage, liquor, confectionary, enteric food, pickled food or diet food containing high-purity sugar sugar stevioside prepared by the method of any one of claims 1 to 7. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950004524A 1995-03-06 1995-03-06 Method for preparing high purity sugar transition stevioside KR960034427A (en)

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KR1019950004524A KR960034427A (en) 1995-03-06 1995-03-06 Method for preparing high purity sugar transition stevioside

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KR960034427A true KR960034427A (en) 1996-10-22

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