KR960034414A - A novel SWI3 gene (SRG3) isolated from a mouse and a protein expressed therefrom - Google Patents

A novel SWI3 gene (SRG3) isolated from a mouse and a protein expressed therefrom Download PDF

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KR960034414A
KR960034414A KR1019960033288A KR19960033288A KR960034414A KR 960034414 A KR960034414 A KR 960034414A KR 1019960033288 A KR1019960033288 A KR 1019960033288A KR 19960033288 A KR19960033288 A KR 19960033288A KR 960034414 A KR960034414 A KR 960034414A
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srg3
gene
protein
mouse
swi3
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KR100191125B1 (en
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성노현
박상대
전성호
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성노현
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity

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Abstract

본 발명은 사카로마이시즈 세레비지애(Saccharomyces cerevisiae)의 SWI3 유전자와 유추 아미노산 서열에 있어 매우 높은 상동성을 나타내는 마우스 유래의 신규한 SRG3(SWI3-related gene)유전자, 그로부터 유추되는 아미노산 서열을 가지는 SRG3 단백질 및 전기 유전자로부터 발현되어 마우스의 SW12계 단백질과 복합체 SRG3 단백질에 관한 것이다. 본 발명의 SRG3 유전자는 1101개의 아미노산을 암호화하는 3303bp의 재방해독틀을 포함하고 있다. 아울러, SRG3 유전자로부터 유추되는 아미노산 서열을 분석한 결과, N-말단 부위는 아스파라긴산과 글루탐산이 풍부하여 산성을 띠고, 아르기닌과 라이신이 풍부한 염기성 부위는 C-말단의 로이신-지퍼 모티브와 인접한 곳에 위치하며, C-말단 부위는 프롤린과 글루타민이 풍부한 것으로 확인되었으며, 또한 N-글리코실화 부위 및 인산화 부위가 존재하는 것으로 확인되었다. 본 발명에서 분리한 마우스의 SRG3 유전자는 SRG3 단백질의 발현 및 분리등에 이용될 수 있으며, 나아가 사람의 SWI3계 유전자 분리에 이용될 수도 있다. 또한 SRG3 단백질을 T세포 분화의 주요 장소인 흉선에서 높게 발현되므로, 면역 조절인자의 탐색 및 개발에 이용될 수도 있다.The present invention relates to a novel SRG3 (SWI3-related gene) gene derived from a mouse, which shows a very high homology with the SWI3 gene of Saccharomyces cerevisiae in a similar amino acid sequence, and an amino acid sequence deduced therefrom SRG3 protein and a complex SRG3 protein expressed from an electric gene and expressed in mouse SW12 system protein. The SRG3 gene of the present invention includes a 3303 bp re-decoding frame encoding 1101 amino acids. As a result of analysis of the amino acid sequence deduced from the SRG3 gene, the N-terminal region is acidic due to abundance of aspartic acid and glutamic acid, and the basic region rich in arginine and lysine is located adjacent to the C- terminal leucine-zipper motif , The C-terminal region was found to be rich in proline and glutamine, and N-glycosylation sites and phosphorylation sites were also found to be present. The SRG3 gene of the mouse isolated in the present invention can be used for expression and isolation of SRG3 protein and further can be used for human SWI3 gene isolation. In addition, SRG3 protein is highly expressed in the thymus, which is the main site of T cell differentiation, and may be used for the search and development of immunoregulatory factors.

Description

마우스에서 분리한 신규의 SWI3계 유전자(SRG3) 및 그로부터 발현되는 단백질A novel SWI3 gene (SRG3) isolated from a mouse and a protein expressed therefrom

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 SRG3 유전자 및 3가지 클론의 제한효소 지도이다, 제2도는 SRG3 유전자의 염기서열 및 그로부터 유추되는 아미노산 서열을 나타낸다, 제3도는 SRG3 단백질과 사카로마이시즈 세레비지애(Saccharomyces cerevisiae)SWI3 단백질과의 아미노산 서열의 상동성을 나타내는 영역을 도시한 그림이다, 제4(A)도는 마우스의 여러가지 조직에서의 SRG3 유전자의 발현을 조사한 노던 블럿(Nothern blot) 분석 결과를 나타내는 사진이다, 제4(B)도는 T 세포 및 B 세포에서의 SRG3 유전자의 발현을 조사한 너던블럿(Nothern blot) 분석 결과를 나타내는 사진이다, 제5도는 SRG3 단백질의 면역학적 블럿(immunoblot) 및 면역학적 침전(immunoprecipitation) 결과를 나타내는 사진이다.FIG. 1 is a restriction map of the SRG3 gene and three clones. FIG. 2 shows the nucleotide sequence of the SRG3 gene and the amino acid sequence deduced therefrom. FIG. 3 shows the sequence of the SRG3 protein and the Saccharomyces cerevisiae SWI3 FIG. 4 (A) is a photograph showing the results of Northern blot analysis of SRG3 gene expression in various tissues of mice. FIG. 4 (B) shows the results of Nothern blot analysis of SRG3 gene expression in T cells and B cells. FIG. 5 shows immunoblot and immunoprecipitation results of SRG3 protein FIG.

Claims (8)

마우스에서 분리한 하기와 같은 염기서열을 가지는 SRG3(SWI3-related gene) cDNA 및 그의 기능적 등가물: gene) cDNA 및 그의 기능적 등가물.SRG3 (SWI3-related gene) cDNA and its functional equivalent (gene) cDNA and functional equivalent thereof having the following base sequence isolated from a mouse. 제1항에 있어서, 마우스의 흉선에서 분리되는 것을 특징으로 하는 SRG3(SWI3-related gene) cDNA 및 그의 기능적 등가물.The SRG3 (SWI3-related gene) cDNA and functional equivalent thereof according to claim 1, which is isolated from the thymus of a mouse. 제1항의 염기서열로부터 유추되는 하기와 같은 아미노산 서열 또는 이와 기능적으로 동등한 아미노산 서열을 가지는 SRG3 단백질:A SRG3 protein having an amino acid sequence or a functionally equivalent amino acid sequence derived from the nucleotide sequence of claim 1, 제3항에 있어서, 아미노산 #860-#951의 로이신-지퍼 모티브(leucin-zipper motif)의 N-말단에 아르기닌 및 라이신이 풍부한 염기성 부위가 존재하는 것을 특징으로 하는 SRG3 단백질 및 그의 기능적 등가물.4. The SRG3 protein and functional equivalent thereof according to claim 3, characterized in that arginine and lysine-rich basic sites are present at the N-terminus of the leucin-zipper motif of amino acids # 860- # 951. 제3항에 있어서, C-말단에 플롤린 및 글루타민이 풍부한 것을 특징으로 하는 SRG3 단백질 및 그의 기능적 등가물.4. The SRG3 protein of claim 3 wherein the C-terminus is rich in follin and glutamine, and a functional equivalent thereof. 제3항에 있어서, N-글리코실화 위치 및 인산화 위치가 존재하는 것을 특징으로 하는 SRG3 단백질 및 그의 기능적 등가물.4. The SRG3 protein of claim 3, wherein the N-glycosylation site and the phosphorylation site are present, and a functional equivalent thereof. 제1항의 유전자로부터 발현된 170kDa의 분자량을 가지는 SRG3 단백질.A SRG3 protein having a molecular weight of 170 kDa expressed from the gene of claim 1. 제7항에 있어서, 마우스의 SWI2계 단백질 또는 다른 SWI/SNF 단백질과 복합체를 형성하는 것을 특징으로 하는 SRG3 단백질.8. The SRG3 protein of claim 7, which complexes with mouse SWI2 family proteins or other SWI / SNF proteins. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960033288A 1996-08-10 1996-08-10 New sw13-related gene purified from mouse and the protein expressed from that KR100191125B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100460228B1 (en) * 2001-08-10 2004-12-14 이화여자대학교 산학협력단 LAZip, a leucine-zipper protein, mediating T cell activation and gene thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100460228B1 (en) * 2001-08-10 2004-12-14 이화여자대학교 산학협력단 LAZip, a leucine-zipper protein, mediating T cell activation and gene thereof

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