KR960031594A - Polymeric conjugates of recombinant human superoxide disulfase (rhSOD) and methods for preparing same - Google Patents

Polymeric conjugates of recombinant human superoxide disulfase (rhSOD) and methods for preparing same Download PDF

Info

Publication number
KR960031594A
KR960031594A KR1019950003217A KR19950003217A KR960031594A KR 960031594 A KR960031594 A KR 960031594A KR 1019950003217 A KR1019950003217 A KR 1019950003217A KR 19950003217 A KR19950003217 A KR 19950003217A KR 960031594 A KR960031594 A KR 960031594A
Authority
KR
South Korea
Prior art keywords
rhsod
recombinant human
disulfase
derivative
human superoxide
Prior art date
Application number
KR1019950003217A
Other languages
Korean (ko)
Other versions
KR100360946B1 (en
Inventor
김동수
김규돈
Original Assignee
성재갑
주식회사 Lg 화학
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 성재갑, 주식회사 Lg 화학 filed Critical 성재갑
Priority to KR1019950003217A priority Critical patent/KR100360946B1/en
Publication of KR960031594A publication Critical patent/KR960031594A/en
Application granted granted Critical
Publication of KR100360946B1 publication Critical patent/KR100360946B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
    • C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
    • C12Y115/01001—Superoxide dismutase (1.15.1.1)

Abstract

본 발명은 제조합 인간 초산화물 불균화효소(rhSOD: recombinant human Superoxide Dismutase)의 혈중반감기를 증가시키기 위해 그의 고분자 결합체를 형성하는 방법에 관한 것으로, 합성 고분자의 히드록시기를 카르복실기로 산화시켜 고분자의 카르복실 유도체를 생성시키고, 상기 유도체를 활성화시키고, 상기 활성화된 유도체를 rhSOD에 결합시켜 제조된 rhSOD-합성 고분자 결합체는 rhSOD만의 경우에 비해 활성은 그대로 유지하면서도 반감기는 휠씬 증가되어 임상치료제로서 유용하게 사용될 수 있다.The present invention relates to a method for forming a polymer conjugate to increase the blood half-life of recombinant human Superoxide Dismutase (rhSOD). The rhSOD-synthetic polymer conjugate prepared by generating a derivative, activating the derivative, and binding the activated derivative to rhSOD can be usefully used as a clinical therapeutic agent because its half-life is greatly increased while maintaining the activity as compared to rhSOD alone. have.

Description

재조합 인간 초-산화물 불균화효소(rhSOD)의 고분자 결합체 및 이를 제조하는 방법Polymeric conjugates of recombinant human superoxide disulfase (rhSOD) and methods for preparing same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도 (A) 및 (B)는 각각 폴리에틸렌글리콜(PEG)과 그의 카르복실 유도체인 PEG-COOH를 IR로 분석한 결과이고,1 (A) and (B) show the results of IR analysis of PEG-COOH, which is polyethylene glycol (PEG) and carboxyl derivatives thereof, respectively,

제2도는 PEG-rhSOD 결합체를 폴리아크릴아미드 겔 전기영동한 결과이고,2 is the result of polyacrylamide gel electrophoresis of PEG-rhSOD conjugate,

제3도는 PEG-rhSOD결합체의 분자량의 변화를 HPLC로 분석한 결과이다.FIG. 3 shows the results of HPLC analysis of changes in molecular weight of PEG-rhSOD conjugates.

Claims (4)

히드록시기를 갖는 합성 고분자의 히드록시기를 산화시켜 카르복실 유도체를 생성시키고, 상기 유도체를 활성화시키고, 상기 활성화된 유도체를 재조합 인간 초-산화물 불규화효소(rhSOD)에 결합시키는 단계를 포함하는, 재조합 인간 초-산화물 불균화효소와 합성 고분자의 결합체를 제조하는 방법.Oxidizing the hydroxy group of the synthetic polymer having a hydroxy group to produce a carboxyl derivative, activating the derivative, and binding the activated derivative to recombinant human superoxide disulfase (rhSOD). -A method of preparing a combination of an oxide disproportionase and a synthetic polymer. 제1항에 있어서, 상기 합성 고분자가 폴리에틸렌 글리콜 또는 메톡시 폴리에틸렌 글리콜인 방법.The method of claim 1 wherein the synthetic polymer is polyethylene glycol or methoxy polyethylene glycol. 제1항에 있어서, 상기 카르복실 유도체의 활성화를, 트리메틸아세틸클로라이드를 사용하여 수행하는 방법.The method of claim 1, wherein the activation of the carboxyl derivative is carried out using trimethylacetylchloride. 제1항의 방법에 의해 제조된 재조합 인간 초-산화물 불균화효소와 합성 고분자의 결합체.A combination of a recombinant human superoxide disulfase and a synthetic polymer prepared by the method of claim 1. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950003217A 1995-02-20 1995-02-20 Polymer conjugate of recombinant human superoxide dismutase(rhsod) and preparation method thereof KR100360946B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950003217A KR100360946B1 (en) 1995-02-20 1995-02-20 Polymer conjugate of recombinant human superoxide dismutase(rhsod) and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950003217A KR100360946B1 (en) 1995-02-20 1995-02-20 Polymer conjugate of recombinant human superoxide dismutase(rhsod) and preparation method thereof

Publications (2)

Publication Number Publication Date
KR960031594A true KR960031594A (en) 1996-09-17
KR100360946B1 KR100360946B1 (en) 2003-01-24

Family

ID=37490552

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950003217A KR100360946B1 (en) 1995-02-20 1995-02-20 Polymer conjugate of recombinant human superoxide dismutase(rhsod) and preparation method thereof

Country Status (1)

Country Link
KR (1) KR100360946B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101749665B1 (en) 2014-10-21 2017-06-21 가톨릭대학교 산학협력단 Composition for stabilizing EC-SOD and method of stabilizing EC-SOD using the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4814098A (en) * 1986-09-06 1989-03-21 Bellex Corporation Magnetic material-physiologically active substance conjugate

Also Published As

Publication number Publication date
KR100360946B1 (en) 2003-01-24

Similar Documents

Publication Publication Date Title
ATE88898T1 (en) CD4 POLYPEPTIDE DERIVATIVES.
KR890000101A (en) How to improve the treatment of banwol tissue and therapeutic drugs
ATE211467T1 (en) 4-AMINO DERIVATIVES OF MYCOPHENOLIC ACID WITH IMMUNE SUPPRESSIVE EFFECTS
TR200101751T2 (en) Polyol-IFN-beta conjugates
ATE111341T1 (en) HAIR COSMETIC PREPARATIONS.
ATE263579T1 (en) RELEASE OF POLYETHYLENE GLYCOL MOLECULE CONJUGATES FROM DEGRADABLE HYDROGEL
DK0973819T3 (en) Non-antigenic branched polymer conjugates
DE3765146D1 (en) CROSSLINKED CARBOXYL GROUPS OF COPOLYMERS USED AS THICKENING AGENTS IN AQUEOUS SYSTEMS AND THEIR PRODUCTION THEREOF.
NO933432D0 (en) GAS CONTROLS
EA200100939A1 (en) PROLONGED OPERATING INSULINOTROPIC PEPTIDES
DE69430317D1 (en) WATER-SOLUBLE ACTIVE SULFONES FROM POLYETHYLENE GLYCOLES
ATE299012T1 (en) COSMETIC COMPOSITION FOR SKIN REGENERATION WITHOUT SKIN IRRITATION
CY1106886T1 (en) NEUBLASTIN POLYMER COGNITION PRODUCTS AND METHODS OF USE
ATE197903T1 (en) PEPTIDE-HYDROCARBON CONJUGATES WHICH EFFECT T-CELL IMMUNITY
ATE210127T1 (en) 6-SUBSTITUTED MYCOPHENOLIC ACID DERIVATIVES WITH IMMUNE SUPPRESSIVE EFFECTS
DK0450628T3 (en) Saccharide-mediated, water-soluble proteins
KR940018087A (en) 6-chloro-5-fluoro-3- (2-tenoyl) -2-oxindol-I-carboxamide as analgesic and anti-inflammatory agent
KR960031594A (en) Polymeric conjugates of recombinant human superoxide disulfase (rhSOD) and methods for preparing same
ATE168115T1 (en) DISSOLVING PROTEINS IN ACTIVE FORM
ATE210126T1 (en) 4-AMINO-6-SUBSTITUTED MYCOPHENOLIC ACID DERIVATIVES WITH IMMUNOSUPRESSIVE EFFECTS
FR2808443B1 (en) MOISTURIZING COSMETIC AND / OR DERMATOLOGICAL COMPOSITION WITH IMPROVED STABILITY
DE3677511D1 (en) OLIGOSACCHARIDES, METHOD FOR THEIR PRODUCTION AND THEIR USE.
ATE202922T1 (en) OPHTHALMIC CROMOLYNE GEL PREPARATION
DE69724328D1 (en) Conjugates of an interleukin-2 receptor specific recognizing factor with ribonuclease
PT92379A (en) Method for the synthesis of polypeptide derivatives

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
N231 Notification of change of applicant
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20060915

Year of fee payment: 5

LAPS Lapse due to unpaid annual fee