KR960023054A - Method for Purifying Recombinant Human Erythropoietin - Google Patents

Method for Purifying Recombinant Human Erythropoietin Download PDF

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Publication number
KR960023054A
KR960023054A KR1019940032993A KR19940032993A KR960023054A KR 960023054 A KR960023054 A KR 960023054A KR 1019940032993 A KR1019940032993 A KR 1019940032993A KR 19940032993 A KR19940032993 A KR 19940032993A KR 960023054 A KR960023054 A KR 960023054A
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KR
South Korea
Prior art keywords
column
human erythropoietin
recombinant human
ion exchange
exchange resin
Prior art date
Application number
KR1019940032993A
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Korean (ko)
Other versions
KR0162517B1 (en
Inventor
하병집
김현수
오명석
이동억
유리안
김석준
박완제
우구
Original Assignee
김정순
제일제당 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 김정순, 제일제당 주식회사 filed Critical 김정순
Priority to KR1019940032993A priority Critical patent/KR0162517B1/en
Publication of KR960023054A publication Critical patent/KR960023054A/en
Application granted granted Critical
Publication of KR0162517B1 publication Critical patent/KR0162517B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 블루세파로스 컬럼, 하이드록시아파타이트 컬럼, 이온교환수지 컬럼을 이용한 크로마토그래피 법을 이용하여 생물학적 활성을 갖는 인간 EPO를 생산 및 분비할 수 있도록 EPO를 코드화하는 유전자로 형질전환된 세포주의 배양액으로부터 인간 EPO단백질을 순수하게 정제하는 방법에 관한 것이다.The present invention provides a culture of a cell line transformed with a gene encoding an EPO to produce and secrete biologically active human EPO using a chromatographic method using a blue sepharose column, a hydroxyapatite column, and an ion exchange resin column. The present invention relates to a method for purely purifying human EPO protein.

Description

재조합 인간 에리스로포이에틴의 정제 방법Method for Purifying Recombinant Human Erythropoietin

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (3)

에리스로포이에틴을 함유하는 세포배양액을 블루 세파로스 컬럼, 하이드록시아파타이트 컬럼, 이온교환수지 컬럼에 연속적으로 통과시킴을 특징으로 하는 인간 에리스로포이에틴의 분리 및 정제 방법.A method of isolating and purifying human erythropoietin characterized by passing a cell culture solution containing erythropoietin continuously through a blue sepharose column, a hydroxyapatite column and an ion exchange resin column. 제1항에 있어서, 세포배양액이 CHOEp-cfc33 세포의 배양액임을 특징으로 하는 방법.The method of claim 1, wherein the cell culture medium is a culture medium of CHOEp-cfc33 cells. 제1항에 있어서, 이온교환수지 컬럼이 QAE 세파로스 컬럼 및 SP-토요펄 컬럼 중에서 선택되는 방법.The method of claim 1 wherein the ion exchange resin column is selected from a QAE Sepharose column and an SP-Toyopearl column. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940032993A 1994-12-06 1994-12-06 Method for purifying recombinant human erythropoietin KR0162517B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940032993A KR0162517B1 (en) 1994-12-06 1994-12-06 Method for purifying recombinant human erythropoietin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940032993A KR0162517B1 (en) 1994-12-06 1994-12-06 Method for purifying recombinant human erythropoietin

Publications (2)

Publication Number Publication Date
KR960023054A true KR960023054A (en) 1996-07-18
KR0162517B1 KR0162517B1 (en) 1998-11-16

Family

ID=19400492

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940032993A KR0162517B1 (en) 1994-12-06 1994-12-06 Method for purifying recombinant human erythropoietin

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KR (1) KR0162517B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100900013B1 (en) * 2007-12-04 2009-05-29 씨제이제일제당 (주) Method of purifying recombinant human erythropoietin from a fluid

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Publication number Publication date
KR0162517B1 (en) 1998-11-16

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