KR960021041A - How to Extract Sekonoganoside from Gold Silver Coins - Google Patents

How to Extract Sekonoganoside from Gold Silver Coins Download PDF

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Publication number
KR960021041A
KR960021041A KR1019940037731A KR19940037731A KR960021041A KR 960021041 A KR960021041 A KR 960021041A KR 1019940037731 A KR1019940037731 A KR 1019940037731A KR 19940037731 A KR19940037731 A KR 19940037731A KR 960021041 A KR960021041 A KR 960021041A
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KR
South Korea
Prior art keywords
extract
gold silver
water
sekonoganoside
high pressure
Prior art date
Application number
KR1019940037731A
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Korean (ko)
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KR100346609B1 (en
Inventor
김훈주
김순하
박은숙
김준
김정민
Original Assignee
성재갑
주식회사 Lg 화학
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Priority to KR1019940037731A priority Critical patent/KR100346609B1/en
Publication of KR960021041A publication Critical patent/KR960021041A/en
Application granted granted Critical
Publication of KR100346609B1 publication Critical patent/KR100346609B1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
    • A61K31/341—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide not condensed with another ring, e.g. ranitidine, furosemide, bufetolol, muscarine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 금은화로부터 세코노가노사이드를 추출하는 방법에 관한 것으로, 금은화를 수성용매로 추출하여 얻어진 추출물을 소수성 페닐세파로즈 크로마토그라피 및 수집용 역상 고압 액체 크로마토그래피를 수행하여 얻어진 세코노가노사이드는 B 형 간염 표면 항원 생성에 대한 억제 효과가 있으므로, 이를 유효활성 성분으로 포함하는 약학 조성물은 B형 간염 치료제로서 매우 유용하게 사용될 수 있다.The present invention relates to a method for extracting seconoganoside from gold silver, wherein the extract obtained by extracting gold silver with an aqueous solvent is subjected to hydrophobic phenyl sepharose chromatography and reverse phase high pressure liquid chromatography for collection. Since there is an inhibitory effect on the production of hepatitis B surface antigen, pharmaceutical compositions comprising it as an active ingredient can be very useful as a hepatitis B therapeutic agent.

Description

금은화로부터 세코노가노사이드를 추출하는 방법How to Extract Sekonoganoside from Gold Silver Coins

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (4)

금은화를 수성용매로 추출하여 얻어진 추출물을 소수성 페닐세파로즈 크로마토그라피 및 수집용 역상 고압 액체 크로마토그라피를 수행하여 세코노가노사이드에 해당하는 구획을 수집하는 것을 특징으로 하는 금은화로부터 세코노가노사이드를 추출하는 방법.Extract the sekonoganoside from the gold silver coin, wherein the extract obtained by extracting the silver coin with an aqueous solvent is subjected to hydrophobic phenylsepharose chromatography and collecting reversed phase high pressure liquid chromatography to collect the compartments corresponding to the seconoganoside. How to. 제1항에 있어서, 상기 수성용매로 물을 사용하여, 60 내지 90℃에서 12 내지 48시간 동안 물중탕 추출하는 것을 특징을 하는 방법.The method according to claim 1, wherein the water is extracted in a water bath at 60 to 90 ° C. for 12 to 48 hours using water as the aqueous solvent. 제1항 또는 제2항에 있어서, 상기 소수성 페닐세파로즈 크로마토그라피 수행시, 10%(v/v) 아세토니트릴로 등용매 용출하는 것을 특징으로 하는 방법.The method according to claim 1 or 2, wherein the isocratic elution with 10% (v / v) acetonitrile is carried out during the hydrophobic phenylsepharose chromatography. 제1항 또는 제2항에 있어서, 수집용 역상 고압 액체 크로마토그래피 수행시 C18 컬럼을 사용하고, 용출용매로 0.1% 트리플루오로아세트산이 들어있는 아세토니트릴 : 물(9:91) (v/v)을 사용하여 등용매 용출시키는 것을 특징으로 하는 방법.The acetonitrile: water (9:91) (v / v) according to claim 1 or 2, using a C18 column for performing reverse phase high pressure liquid chromatography for collection, and containing 0.1% trifluoroacetic acid as an eluting solvent. Isocratic elution using the above method. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940037731A 1994-12-28 1994-12-28 Method for extracting seconoganoside from lonicerae flos KR100346609B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940037731A KR100346609B1 (en) 1994-12-28 1994-12-28 Method for extracting seconoganoside from lonicerae flos

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940037731A KR100346609B1 (en) 1994-12-28 1994-12-28 Method for extracting seconoganoside from lonicerae flos

Publications (2)

Publication Number Publication Date
KR960021041A true KR960021041A (en) 1996-07-18
KR100346609B1 KR100346609B1 (en) 2002-11-18

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ID=37488705

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940037731A KR100346609B1 (en) 1994-12-28 1994-12-28 Method for extracting seconoganoside from lonicerae flos

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Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5679623A (en) * 1979-12-03 1981-06-30 Kitasato Inst:The Interferon inducting agent and its preparation
US5178865A (en) * 1990-06-19 1993-01-12 Cedars-Sinai Medical Center Chinese herbal extracts in the treatment of hiv related disease in vitro
JP3007665B2 (en) * 1990-09-18 2000-02-07 日清製粉株式会社 Preventive and therapeutic agent for coronavirus infection and disinfectant
KR940013527A (en) * 1992-12-30 1994-07-15 윤선중 Chinese herbal medicine preparation for cancer treatment
US5837257A (en) * 1996-07-09 1998-11-17 Sage R&D Use of plant extracts for treatment of HIV, HCV and HBV infections

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