KR960009321B1 - Making method of l-threonin by recombinant microorganism - Google Patents

Making method of l-threonin by recombinant microorganism Download PDF

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Publication number
KR960009321B1
KR960009321B1 KR92024732A KR920024732A KR960009321B1 KR 960009321 B1 KR960009321 B1 KR 960009321B1 KR 92024732 A KR92024732 A KR 92024732A KR 920024732 A KR920024732 A KR 920024732A KR 960009321 B1 KR960009321 B1 KR 960009321B1
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KR
South Korea
Prior art keywords
threonin
coli
recombinant
making method
recombinant microorganism
Prior art date
Application number
KR92024732A
Other languages
Korean (ko)
Other versions
KR940014793A (en
Inventor
Jin-Ho Lee
Hyun-Hwan Lee
Kang-Ho Lee
Jae-Kwon Kim
Kap-Soo Noh
Hyung-Soo Chon
Original Assignee
Cheil Foods & Chem
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Publication date
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Priority to KR92024732A priority Critical patent/KR960009321B1/en
Publication of KR940014793A publication Critical patent/KR940014793A/en
Application granted granted Critical
Publication of KR960009321B1 publication Critical patent/KR960009321B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L-threonin is prepared by (a) making recombinant plasmid PAT94 with threonin operon derived from E-coli and par region concerned with plasmid division; (b) converting the character of E-coli TF4076 into recombinant E-coli whose expression isn't suppressed by threonin and isoleucine and whose biosynthetic enzyme is inhibited by threonin with recombinant plasmid PAT94 ; (c) culturing the recombinant E-coli; (d) accumulating L-threonin in the medium; (e) recovering L-threonin from the medium.
KR92024732A 1992-12-17 1992-12-17 Making method of l-threonin by recombinant microorganism KR960009321B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR92024732A KR960009321B1 (en) 1992-12-17 1992-12-17 Making method of l-threonin by recombinant microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR92024732A KR960009321B1 (en) 1992-12-17 1992-12-17 Making method of l-threonin by recombinant microorganism

Publications (2)

Publication Number Publication Date
KR940014793A KR940014793A (en) 1994-07-19
KR960009321B1 true KR960009321B1 (en) 1996-07-18

Family

ID=19345989

Family Applications (1)

Application Number Title Priority Date Filing Date
KR92024732A KR960009321B1 (en) 1992-12-17 1992-12-17 Making method of l-threonin by recombinant microorganism

Country Status (1)

Country Link
KR (1) KR960009321B1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7368266B2 (en) 2001-02-13 2008-05-06 Cj Corporation Method for L-threonine production
KR100397423B1 (en) 2001-02-13 2003-09-13 씨제이 주식회사 Process for producing L-threonine
KR101135325B1 (en) * 2004-11-29 2012-04-17 두산인프라코어 주식회사 Vibration preventing instrument attached a turret

Also Published As

Publication number Publication date
KR940014793A (en) 1994-07-19

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