KR960007777A - Method for preparing erythropoietin - Google Patents

Method for preparing erythropoietin Download PDF

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Publication number
KR960007777A
KR960007777A KR1019940020595A KR19940020595A KR960007777A KR 960007777 A KR960007777 A KR 960007777A KR 1019940020595 A KR1019940020595 A KR 1019940020595A KR 19940020595 A KR19940020595 A KR 19940020595A KR 960007777 A KR960007777 A KR 960007777A
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KR
South Korea
Prior art keywords
human
human erythropoietin
vector
erythropoietin
molonymurine
Prior art date
Application number
KR1019940020595A
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Korean (ko)
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KR970009952B1 (en
Inventor
이동억
김현수
박완제
김석준
유리안
하병집
유왕돈
김수옥
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김정순
제일제당 주식회사
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Priority to KR94020595A priority Critical patent/KR970009952B1/en
Publication of KR960007777A publication Critical patent/KR960007777A/en
Application granted granted Critical
Publication of KR970009952B1 publication Critical patent/KR970009952B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 유전자 재조합 기술에 의하여 SV40 어얼리 프로모터와 몰로니뮤린 류케미아바이러스의 LTR(long terminal repeat)인핸서에 연결시킴으로써 매우 높은 형질발현 역가를 제공하도록 만들어진 인간 에리스로포이에틴 게놈 유전자를 형질발현시켜 생물학적 활성을 갖는 인간 에리스로포이에틴을 고수율로 제조하는 방법에 관한 것이다.The present invention relates to a long terminal repeat (LTR) enhancer of the SV40 early promoter and molonymurine leukemia virus by gene recombination technology to express human erythropoietin genomic genes designed to provide very high expression expression titers for biological activity. The present invention relates to a method for producing human erythropoietin having high yield.

Description

에리스로포이에틴의 제조방법Method for preparing erythropoietin

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (3)

크기가 2.4kb이며 인간 에리스로포이틴을 코드화하는 인간 게놈유전자를 SV40 어얼리프로모터와 몰로니뮤린류케미아바이러스의 LTR(long terminal repeat)인핸서에 연결하여 벡터 pSVEp2neo(가락번호;KFCC-10831)를 제조하고 이 벡터로 숙주세포를 형질전환 시킨후, 형질전환된 세포를 배양하여 인간 에리스로포이에틴을 제조하는 방법.A vector pSVEp2neo (paragraph; KFCC-10831) was prepared by connecting a human genome gene of 2.4 kb in size and encoding a human erythropoinin to a long terminal repeat (LTR) enhancer of an SV40 early promoter and molonymurine leucine virus. And transforming the host cell with the vector, and then culturing the transformed cell to produce human erythropoietin. 제1항에 있어서 숙주페수주가 CHO(tk-) 또는 COS-1 세포주임을 특징으로 하는 방법.The method according to claim 1, wherein the host effusion strain is a CHO (tk-) or COS-1 cell line. 제1항에 있어서 인간 에리스로포이에틴을 코드화하는 인간 게놈유전자가 하기와 같은 염기서열로 구성됨을 특징으로 하는 방법.The method of claim 1, wherein the human genomic gene encoding human erythropoietin is composed of the following nucleotide sequences. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR94020595A 1994-08-20 1994-08-20 Process for preparing erythropoietin KR970009952B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR94020595A KR970009952B1 (en) 1994-08-20 1994-08-20 Process for preparing erythropoietin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR94020595A KR970009952B1 (en) 1994-08-20 1994-08-20 Process for preparing erythropoietin

Publications (2)

Publication Number Publication Date
KR960007777A true KR960007777A (en) 1996-03-22
KR970009952B1 KR970009952B1 (en) 1997-06-19

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ID=19390802

Family Applications (1)

Application Number Title Priority Date Filing Date
KR94020595A KR970009952B1 (en) 1994-08-20 1994-08-20 Process for preparing erythropoietin

Country Status (1)

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KR (1) KR970009952B1 (en)

Also Published As

Publication number Publication date
KR970009952B1 (en) 1997-06-19

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