KR960006940A - Production method of Newcastle disease vaccine using animal cell culture method - Google Patents

Production method of Newcastle disease vaccine using animal cell culture method Download PDF

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Publication number
KR960006940A
KR960006940A KR1019940021652A KR19940021652A KR960006940A KR 960006940 A KR960006940 A KR 960006940A KR 1019940021652 A KR1019940021652 A KR 1019940021652A KR 19940021652 A KR19940021652 A KR 19940021652A KR 960006940 A KR960006940 A KR 960006940A
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KR
South Korea
Prior art keywords
cell culture
animal cell
virus
newcastle disease
animal
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KR1019940021652A
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Korean (ko)
Inventor
강계원
김정희
이균민
임현수
Original Assignee
심상철
한국과학기술원
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Application filed by 심상철, 한국과학기술원 filed Critical 심상철
Priority to KR1019940021652A priority Critical patent/KR960006940A/en
Publication of KR960006940A publication Critical patent/KR960006940A/en

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Abstract

본 발명은 동물세포 배양법을 통하여 뉴캣슬병 백신을 생산하는 방법을 제공한다. 본 발명의 생산방법은 동물 세포를 조잭배향 배지에서 배양하여 동물세포 배양액을 얻는 단계(a); 동물세포 배양액에 뉴캣슬병 바이러스를 접종 및 흡착시키고 이어 배양하는 단계(b); 및 전 단계에서 얻은 배양액으로붜 바이러스 감염액을 회수하는 단계(c)를 포함함을 특징으로 하며, 본 발명에서 사용 가능한 동물세포는 10일령된 계태아의 섬유아세포 또는 vero 세포주이고, 이들 동물세포는 액체배양하거나 또는 표면부작용 담체 또는 다공성 담체를 이용하여 배양할 수 있다. 또한 본 발명에 따르면 상기 (b)단계에서 바이러스를 흡착시키고 1시간 후에 0.5-10.0mM의 EDTA를 첨가하므로써 고농도로 안정되게 백신을 생산할 수 있다.The present invention provides a method for producing Newcastle disease vaccine through animal cell culture. The production method of the present invention comprises the steps of (a) obtaining an animal cell culture by culturing the animal cells in a jackjack orientation medium; Inoculating and adsorbing Newcastle disease virus on animal cell culture and then culturing; And (c) recovering the virus infection solution into the culture broth obtained in the previous step, wherein the animal cells usable in the present invention are fibroblasts or vero cell lines of 10-day-old fetuses, and these animal cells Can be cultured using a liquid culture or using a surface-action carrier or a porous carrier. In addition, according to the present invention, the vaccine can be stably produced at a high concentration by adding 0.5-10.0 mM EDTA after adsorbing the virus in step (b).

Description

동물세포 배양법을 이용한 뉴캣슬병 백신의 생산방법Production method of Newcastle disease vaccine using animal cell culture method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

동물 제포를 조직배양 배지에서 배양하여 동물세포 배양액을 얻는 단계(a); 동물세포 배양액에 뉴캣슬병 바이러스를 접종 및 흡착시키고 이어 배양하는 단계(b); 및 전 단계에서 얻은 배양액으로부터 바이러스 감염액을 회수하는 단계(c)를 포함함을 특징으로 하는 뉴캣슬병 백신의 생산방법.(A) obtaining an animal cell culture by culturing the animal cells in tissue culture medium; Inoculating and adsorbing Newcastle disease virus on animal cell culture and then culturing; And (c) recovering the virus infection solution from the culture broth obtained in the previous step. 제1항에 있어서, 동물세포는 10일령된 계태아의 섬유아세포 또는 vero 세포주임을 특징으로 하는 생산방법.The production method according to claim 1, wherein the animal cells are fibroblasts or vero cell lines of 10-day-old fetuses. 제1항에 있어서, (a)단계에서 동물세포 배양은 액체배양에 의해, 또는 표면부착용 담체 또는 다공성 담체를 이용하여 실시함을 특징으로 하는 생산방법.[Claim 2] The production method according to claim 1, wherein the animal cell culture in step (a) is performed by liquid culture or by using a surface-attached carrier or a porous carrier. 제1항에 있어서, (b)단계에서 바이러스를 흡착시키고 1시간 후에 0.5-10.0mM의 EDTA를 첨가함을 특징으로 하는 생산방법.The method according to claim 1, wherein in step (b), 0.5-10.0 mM EDTA is added after 1 hour of adsorbing the virus. 제1항에 있어서, (b)단계에서 바이러스는 0.01-1.0MOI(Multiplicity of Infection)의 양으로 접종됨을 특징으로 하는 생산방법.The method of claim 1, wherein in step (b), the virus is inoculated in an amount of 0.01-1.0 MOI (Multiplicity of Infection). ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940021652A 1994-08-30 1994-08-30 Production method of Newcastle disease vaccine using animal cell culture method KR960006940A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940021652A KR960006940A (en) 1994-08-30 1994-08-30 Production method of Newcastle disease vaccine using animal cell culture method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940021652A KR960006940A (en) 1994-08-30 1994-08-30 Production method of Newcastle disease vaccine using animal cell culture method

Publications (1)

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KR960006940A true KR960006940A (en) 1996-03-22

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