KR960004299B1 - Apparatus and method for measuring of exuviation - Google Patents

Abstract

The apparatus having a conventional resistance tester(1) inside is characterized: in that an anodic plate(2a) and a cathodic plate(2b) are formed in lower side thereof to be exposed to outside; in that the electrode plates(2a)(2b) are insulated by an insulating sponge(3) and each connecting terminal(4a)(4b) of the electrode plates is connected to the resistance tester(1); and in that an electrical properties measured by the resistance tester(1) is displayed on a specific display board(5). Pref. a space(6) is formed between the resistance tester(1) and the insulating sponge(3).

Description

Peeling degree measuring device of skin layer

1 is a conceptual diagram of a peeling degree measuring device of the skin layer as an embodiment of the present invention, (a) is a front view, (b) is a cross-sectional view taken along line AA in Figure (a), (c) is a drawing (a) It shows the back view for.

* Explanation of symbols for main parts of the drawings

1: resistance meter 2a: bipolar plate

2b: negative electrode plate 3: insulating sponge

4a, 4b: electrode connection terminal 5: measuring plate

6: space 7: O point adjustment terminal

8: Current control terminal

The present invention is a method for measuring the degree of peeling of the skin layer using the electrical properties in the process of removing the stratum corneum of the skin for transdermal delivery, skin massage, skin cleaning, etc. of the drug and attached to the peeled skin can measure the degree of peeling It relates to a measuring device configured to be.

The general structure of the skin can be divided into stratum corneum, epidermal layer and dermis layer.

The stratum corneum has a structure of a honey layer in which fats (triglycerides, free fatty acids, cholesterol, phospholipids) surround proteins (mainly keratin), and have a thickness of 10 to 100 μm depending on the site.

The bilayer is formed by the differentiation of the basal cells of the epidermal layer into degenerative cells after about a full period of time, and protects the skin from chemical and physical stimuli from the outside and prevents the penetration of microorganisms.

However, this stratum corneum acts as a large barrier in the transdermal delivery of drugs, reducing the drug delivery efficiency, and in terms of skin beauty, not only causes skin roughness and microbial propagation on the skin surface, but also affects the effects of skin regenerating cosmetics. In order to reduce, conventionally, a method of removing the stratum corneum has been attempted.

Bruce Kari has written Diabetes Vol. In 31 217 (1986), the stratum corneum was partially removed and attempted to deliver a large protein drug such as insulin. Flynn, G.L. et al. J. Pharm. Sci., 70 56 (1981), investigated the removal efficiency of the stratum corneum by adhering and removing the adhesive tape on the skin.

In addition, a cosmetic massage cream is used to polish the stratum corneum by adding micro beads (micro bead).

As described above, the stratum corneum removal method used in the pharmaceutical and cosmetic fields is used in various forms, but the method of measuring the removal amount is very difficult and difficult, and there are many problems in practical use. A brief summary of the measurements currently in use or published in the literature, Marie Loden, J.soc. Cosmet. Chem. At 41, 111 (1990), the stratum corneum was removed with a massage cream, and the moisture evaporation of the skin was measured by using an evaporometer. Another method was performed using a Doppler flowmetry with a laser electrode. Capillary blood flow was imaged in three dimensions to measure and evaluate the stratum corneum.

Balthazar also found Southern Medical J. Vo. 75 7,799 (1982) used the method of observing the extent of damage to the cortex according to the drawings with the naked eye and electron microscopy. Burnette, R. R. et al, J. of Pharm. Sci. 77.2. In order to observe the drug permeability of the epidermal layer in 132 (1988), a method of fluorescence microscopy was used to stain the skin with a fluorescent dye.

However, the measurement method of the extent of the removal of the stratum corneum of the skin layer thus far has problems such as an indirect evaluation method or too large a measuring device, difficult to measure directly on the human skin.

Accordingly, an object of the present invention is to provide a new method and apparatus capable of measuring the extent of stratum corneum removal more easily and quickly.

Hereinafter, the present invention will be described in detail.

In the present invention, the measurement of the peeling degree of the skin layer, using a measuring device configured to enable the resistance measurement by the electrode, the capacitance (resistance) or from these by the electrical characteristic value of the skin area to be measured The measured impedance is measured, and the peeling degree of the skin layer is read from the measured value.

Hereinafter, the present invention will be described in more detail.

As mentioned above, the structure of the skin is roughly divided into stratum corneum, epidermal layer and dermis layer, which are characterized by different electrical property values, that is, resistance or capacitance. In particular, the stratum corneum has both capacitance and resistivity, and the epidermal and dermal layers have relatively low capacitance.

It is thought that the protein, which plays the role of Rsc, the salt content and the fat component that plays the role of Rsc, is present in the stratum corneum, resulting in an electrical impedance-like characteristic.

There is a view that the impedance action is generated when the DC power is supplied to the skin, and thus the charge distribution of the protein molecules in the skin tissue is changed, thereby causing a kind of polarization, which causes a dipole moment, which interrupts the electric flow.

In the present invention, the degree of removal of the epidermal layer is measured using the electrical properties of the epidermal layer. The phenomena resulting from the partial or complete removal of the stratum corneum will result in a decrease in the values of Csc and Rsc. This value changes the overall resistance value and the skin resistance value decreases with the removal of the stratum corneum.

In addition, when measuring the Csc value using a capacitor (Capacitor), the Cs value decreases as the stratum corneum is removed.

On the other hand, as a measuring device capable of effectively implementing the present invention, as shown in FIG. 1, a skin layer peeling degree measuring device having a built-in resistance measuring device 1 is built in. The bottom part has a positive electrode plate 2a and a negative electrode plate ( 2b) is formed to be exposed to the outside, and these electrode plates 2a and 2b are connected to the resistance measuring instrument 1 by respective electrode connection terminals 4a and 4b while being insulated with an insulating sponge 3. In the upper surface portion, a window device configured in a patch form is used to read the electrical characteristic value measured by the resistance measuring instrument 1 through the measurement sign plate 5.

In FIG. 1, reference numeral 6 denotes a space in which the electrode connection terminals 2a and 2b are located, 7 is an O point control terminal, and 8 is a current control terminal.

The measuring device of the present invention is a new device designed only to use the measuring method of the present invention on the skin to measure the conductive electrode plates 2a and 2b to which the positive electrode plate 2a and the negative electrode plate 2b are attached. In close contact with each other, the electrical characteristic values are measured through the resistance meter 1 connected to the electrode plates 2a and 2b.

At this time, the conductive electrode plates (2a, 2b) can be used, such as a conductive metal plate, such as silver, gold, aluminum, iron, carbon or electrically conductive resin plate, the resistance measuring device (1) is a capacitor measuring instrument that can measure the capacitance May be used.

In the measuring apparatus of the present invention, as shown in (b) of FIG. 1, a generally known capacitor or resistance measuring instrument 1 is miniaturized (for example, 6 × 4 × 2 cm), and is used at the bottom thereof. There is an insulating sponge 3 which is isolated from (2a, 2b). The insulating sponge 3 is made of polyethylene, which is used to increase the adhesive effect when the electrodes of the positive electrode plate 2a and the negative electrode plate 2b are adhered to the skin while exhibiting the insulating effect between the electrodes. Use good insulation.

The lower portion of the insulating sponge 3 is attached to the positive electrode plate (2a) and the negative electrode plate (2b) of the thin film form serves to transfer the electrical properties of the skin to the resistance measuring device (1).

In this case, it is preferable to use the electrode having a size of 2.5 × 4 cm and a thickness of 100 μm or less in terms of flexibility and adhesion efficiency when attaching the skin.

On the other hand, as shown in (c), an electrode insulator (a) made of an insulating sponge 3 is formed between each of the electrode plates 2a and 2b, which accurately measures the electrical characteristics of the skin in contact with the electrode. This is to stop the current movement between the positive electrode plate (2a) and the negative electrode plate (2b) that may be caused by moisture or sweat, etc. to form a good reproducible measurement results.

At this time, if the width of the electrode insulator (a), that is, the distance between the positive electrode plate (2a) and the negative electrode plate (2b) is too narrow, an impedence is generated between the electrodes so that a distance of at least 1 cm is required. desirable. The electrical insulator (a) is natural and good to be composed of the same material as the insulating sponge (3), but it is particularly preferable to use a sponge made of cotton or pulp material having good absorption of sweat or moisture.

In addition, the positive electrode plate 2a and the negative electrode plate 2b are connected to each other through the measuring device 1 and the electrode connecting terminals 4a and 4b, respectively, in which connection lines affect the flexibility of the surface of the electrode plates 2a and 2b. Use thin wires that do not give a connection through the insulating sponge (3).

The measuring device of the present invention reduces the flexibility due to the measuring device 1 by placing a space 6 between the measuring device 1 and the insulating sponge 3 as a means for improving skin contact and flexibility of the patch. Minimized. The conductive electrode plates of the positive electrode plate 2a and the negative electrode plate 2b are bonded to the insulating sponge 3 by high frequency fusion or adhesive.

In addition, as shown in FIG. 1A, a measurement sign plate 5 capable of digital display is provided for ease of use, and thus the electrical characteristic values measured by the measuring device 1 can be easily read through the electrode plates 2a and 2b. The O point control terminal 7 and the current control terminal 8 for adjusting the O point at the time of skin measurement are respectively installed.

When using the above-described measuring device according to the present invention, it is good to make the measurement time within 2 to 3 seconds. If the measurement time is longer than this, the conductivity increases due to moisture evaporation of the skin, resulting in uneven measurement.

The present invention can be generally used after removing the stratum corneum in a manner that can remove the electric shaver or blade razor, adhesive tape use, massage cream use, Scrub cream, other skin stratum corneum, in particular, electric razor, tape treatment, An example is given only for the use of the scrub cream.

Next, skin resistance and capacitance were measured by modeling rabbit, pig and human skin, and the results are shown in Table 1.

TABLE 1

Human skin has a denser and thicker stratum corneum than rabbit or pig skin, resulting in stronger electrical resistance. Next, the change of the electrical property value of the skin according to the stratum corneum removal method was tested through the rabbit skin, wherein the measuring device illustrated in FIG.

First, the electrical properties of normal skin were measured, and the changes in the measured values after the stratum corneum was treated with a cellophane adhesive tape and an electric razor are shown in Table 2 below.

As a result of measuring the impedance as a result of Table 2, the value after skin treatment is increased relatively uniformly, and it shows that the effect on moisture which makes the electrical characteristic value unstable is relatively small.

In addition, as a result of measuring the resistance, the treatment with the adhesive tape showed that the normal skin resistance value was uniform, but the resistance value after the treatment did not appear to be uniform. It is thought that the sweat and cell fluid of the skin tissue flowed out of the skin during the tape treatment, resulting in uneven electrical resistance.

On the other hand, electro-shaved skin showed little leakage of sweat and cell fluid during treatment.

As described above, the resistance value can be used in a limited manner in actual use. The resistance value is a factor that is very sensitive to the body fluid secreted from the skin, and it is more preferable to control the skin treatment state by measuring the impedance. As described above, the impedance of skin is a term related to capacitance and resistance, and is relatively insensitive to skin resistance, and depends on capacitance, that is, electrical characteristics from the fat layer, and thus can be widely used in various treatment methods.

TABLE 2

Hereinafter, examples of the present invention will be described.

Example 1

Remove the hair on the back of the white rabbit with an electric clipper and leave it for about 2 days to bring the skin irritation or injury to normal. Determination of the degree of peeling of the stratum corneum is a measuring device of the structure shown in FIG. 1, which is made of a silver plate of 4 × 1.7 cm size. The anode is made with a gap of 0.6 cm so that both ends of the electrode are connected to the capacitor terminal. Used.

First, the silver electrode is placed on the skin where the stratum corneum is not treated, and the capacitance value is measured within 2 seconds.

Then, after rubbing the skin lightly with an electric razor for a certain time, the treatment time interval is 5, 10, 20, 30, 60 seconds, the capacitance value of the skin with each time is measured using a silver electrode.

A drug patch containing insulin was attached to the skin-treated rabbit, and blood glucose was measured by collecting blood from the vein to evaluate the absorption of insulin 6 hours later. As a result, as shown in Table 3, it is confirmed that insulin absorption is changed according to the electrical characteristic value impedance value, so that the treatment of the stratum corneum is uniformly adjusted to the electrical capacitance value.

TABLE 3

Example 2

After massaging evenly for 30, 60, 90, 120 seconds using a massage cream on the skin of the arm of a human, the electrical property value of the skin is measured in the same manner as in Example 1.

The degree of removal of the stratum corneum increases the electrical properties in proportion to the massage time.

TABLE 4

The measurement of the removal amount of the stratum corneum using the electrical property value can reduce the irritation to the skin during the skin treatment and to remove the desired amount of the stratum corneum.

In particular, since the skin of a person is different depending on several factors such as age, race, sex, treatment site, and individual skin quality, it is too much to be used in the conventional method, that is, to process for a certain time or to rely on the user's experience. It is inaccurate and in some cases causes side effects.

However, the present invention can solve this problem by using a new measuring method and device, it is possible to measure the degree of removal of the stratum corneum simply and quickly.

Claims (2)

A skin layer peeling degree measuring device having a built-in resistance measuring device (1), wherein the positive electrode plate (2a) and the negative electrode plate (2b) is exposed to the outside on the bottom portion, these electrode plates (2a, 2b) are insulating sponge Insulated by (3) and connected to the resistance meter (1) by the respective electrode connection terminals (4a, 4b), the upper surface portion, the electrical characteristic value measured by the resistance meter (1) on the specific sign plate (5) Peeling degree measuring device of the skin layer consisting of a patch form to be read through. The measuring device according to claim 2, wherein a space (6) is formed between the resistance meter (1) and the insulating sponge (3).