KR960001110A - Two Agar degrading enzymes with different molecular weights produced by Halophilic Pseudomonas sp. - Google Patents

Two Agar degrading enzymes with different molecular weights produced by Halophilic Pseudomonas sp. Download PDF

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Publication number
KR960001110A
KR960001110A KR1019940013228A KR19940013228A KR960001110A KR 960001110 A KR960001110 A KR 960001110A KR 1019940013228 A KR1019940013228 A KR 1019940013228A KR 19940013228 A KR19940013228 A KR 19940013228A KR 960001110 A KR960001110 A KR 960001110A
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KR
South Korea
Prior art keywords
agar
molecular weights
different molecular
degrading enzymes
pseudomonas
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Application number
KR1019940013228A
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Korean (ko)
Inventor
공재열
공인수
Original Assignee
공재열
공인수
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Priority to KR1019940013228A priority Critical patent/KR960001110A/en
Publication of KR960001110A publication Critical patent/KR960001110A/en

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Abstract

본 발명은 해양으로부터 분리한 미생물인 호염성 슈도모나스 스페시스(Halophilic Pseudomoams sp.)(한국종균협회 KCCM-10052, 기탁일자 1994년 4월 7일)로 부터 생산되는 분자량이 다른 두가지의 한천 분해효소에 관한 것이다. 본 발명에서 사용된 미생물이 생산하는 한천 분해 효소는 한 종류가 아니라 적어도 3개 이상의 분자량, 이온 강도가 다른 한천 분해 효소를 생산하고 있다.The present invention is directed to two agar degrading enzymes having different molecular weights produced from the bactericidal Pseudomoams sp. (Halophilic Pseudomoams sp.) It is about. The agar degrading enzyme produced by the microorganisms used in the present invention is not one but produces at least three or more agar degrading enzymes having different molecular weights and ionic strengths.

Description

호염성 슈도모나스(Halophilic Paeudomonas sp.)가 생산하는 분자량이 다른 두 가지의 한천분해효소(Agar degrading enzyme)Two Agar degrading enzymes with different molecular weights produced by Halophilic Paeudomonas sp.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 해양으로부터 한천분해효소를 생산하는 미생물을 분리하여 한천이 유일한 탄소원으로 첨가된 고체배지상에서 미생물이 생육하면서 한천이 분해된 모습을 보여 주는 사진이다.1 is a photo showing the decomposition of agar as the microorganisms grow on a solid medium to which agar decomposes agar degrading enzymes from the ocean and agar is added as the only carbon source.

제2도는 미생물의 생육과 효소의 생산을 보여주고 있는데 미생물의 생육은 16시간부터 정상기에 들어가 32시간부터는 감소하기 시작하였으며 효소활성은 24시간까지 계속적으로 증가하다가 24시간에서 32시간 사이에는 일정하게 유지되었고 36시간부터 서서히 감소하였다.Figure 2 shows the growth of microorganisms and the production of enzymes. The growth of microorganisms starts at 16 hours and starts to decrease from 32 hours. It was maintained and gradually decreased from 36 hours.

Claims (1)

배지의 조성은 NaCl 30g, KCl 0.7g, MgCl2·6H2O 10.6g, Peptone 5.0g, Yeast extract 1.0g, Ferric citrate 0.1g, Ammonium nitrate 0.0016g, Disodium phosphate 0.008g, Agar 3.0g, DW 11, pH 7.5를 사용하여 호염성 슈도모나스를 20-37℃에서 24-48시간 진탕배양시킨 후 배양액을 10,000rpm에서 10분간 원심분리하여 상층액과 침전물(미생물세포)로 분리한 후 추출한 상층액을 아세톤 처리하여 단백질만 회수한 다음 침전물을 10mM Tris-HCl 완충용액(pH 7.5)에 녹여 투석한 후 DEAE-cellulose 가 채워진 컬럼(column)에서 이온교환크로마토그래피(ion-exchange chromatography)로서 다른형의 한천 분해 효소를 분리하고 활성이 큰 부분을 다시 Sephadex G-200이 채워진 컬럼(column)에서 겔크로마토그래피(Gel chromatography)를 행하여 얻은 분자량이 다른 두가지의 한천 분해 효소.The composition of the medium is NaCl 30g, KCl 0.7g, MgCl 2 · 6H 2 O 10.6g, Peptone 5.0g, Yeast extract 1.0g, Ferric citrate 0.1g, Ammonium nitrate 0.0016g, Disodium phosphate 0.008g, Agar 3.0g, DW 11 , pH 7.5 using basophil Pseudomonas shake culture at 20-37 ℃ 24-48 hours, the culture was centrifuged at 10,000rpm for 10 minutes to separate the supernatant and precipitate (microbial cells) and then extracted supernatant acetone After recovering only the protein by treatment, the precipitate was dissolved in 10 mM Tris-HCl buffer (pH 7.5) and dialyzed, followed by deionization of other types of agar by ion-exchange chromatography on a DEAE-cellulose-filled column. Two agar degrading enzymes with different molecular weights obtained by separating the enzymes and performing the gel chromatography on a column filled with Sephadex G-200. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940013228A 1994-06-10 1994-06-10 Two Agar degrading enzymes with different molecular weights produced by Halophilic Pseudomonas sp. KR960001110A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940013228A KR960001110A (en) 1994-06-10 1994-06-10 Two Agar degrading enzymes with different molecular weights produced by Halophilic Pseudomonas sp.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940013228A KR960001110A (en) 1994-06-10 1994-06-10 Two Agar degrading enzymes with different molecular weights produced by Halophilic Pseudomonas sp.

Publications (1)

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KR960001110A true KR960001110A (en) 1996-01-25

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KR1019940013228A KR960001110A (en) 1994-06-10 1994-06-10 Two Agar degrading enzymes with different molecular weights produced by Halophilic Pseudomonas sp.

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100773626B1 (en) * 2000-10-13 2007-11-05 게스탐프 하르트테크 아베 A bumper arrangement

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100773626B1 (en) * 2000-10-13 2007-11-05 게스탐프 하르트테크 아베 A bumper arrangement

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