KR960000012A - How to grow aloe - Google Patents

How to grow aloe Download PDF

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Publication number
KR960000012A
KR960000012A KR1019940013237A KR19940013237A KR960000012A KR 960000012 A KR960000012 A KR 960000012A KR 1019940013237 A KR1019940013237 A KR 1019940013237A KR 19940013237 A KR19940013237 A KR 19940013237A KR 960000012 A KR960000012 A KR 960000012A
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KR
South Korea
Prior art keywords
aloe vera
kinetin
alpha
aloe
added
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Application number
KR1019940013237A
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Korean (ko)
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KR0133344B1 (en
Inventor
조규현
심창섭
Original Assignee
김정문
주식회사 김정문알로에
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority to KR1019940013237A priority Critical patent/KR0133344B1/en
Publication of KR960000012A publication Critical patent/KR960000012A/en
Application granted granted Critical
Publication of KR0133344B1 publication Critical patent/KR0133344B1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/56—Liliaceae, e.g. Alstroemeria or Lilium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

본 발명은 조직배양 방법을 이용하여 알로에 베라를 대량을 증식시키는 방법을 제공한다. 본 발명의 방법은 알파-나프탈렌초산 또는 2,4-D 10-50uM 및 키네틴 0.5uM을 첨가한 식물 조직 배양용 배지에서 멸균 알로에 베라 조직을 배양하여 알로에 베라의 캘러스를 유도하는 1단계; 및 유도된 캘러스를 알파-나프탈렌 초산 0.5uM과 키네틴 0.5uM을 첨가한 식물조직용 배지에서 배양하여 알로에 베라의 줄기와 뿌리를 형성시키는 2단계를 포함함을 특징으로 한다.The present invention provides a method of mass-proliferating aloe vera using a tissue culture method. The method of the present invention comprises the steps of inducing callus of aloe vera by culturing sterile aloe vera tissue in plant tissue culture medium to which alpha-naphthalene acetate or 2,4-D 10-50 uM and 0.5 kM of kinetin are added; And culturing the induced callus in a medium for plant tissues to which 0.5uM of alpha-naphthalene acetate and 0.5uM of kinetin are added to form stems and roots of aloe vera.

본 발명의 방법에 의하면 알로에의 증식을 제어할 수 있으므로 균질한 품질의 알로에 베라를 대량으로 보다 저렴하게 공급할 수 있다.According to the method of the present invention, since the growth of aloe can be controlled, aloe vera of homogeneous quality can be supplied at a lower cost in large quantities.

Description

알로에의 대량 증식방법How to grow aloe

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명의 방법에 의하여 알로에 조직으로부터 뿌리와 줄기를 재생시킨 것을 보여주는 사진이다.1 is a photograph showing the regeneration of roots and stems from aloe tissue by the method of the present invention.

Claims (2)

알파-나프탈렌초산 또는 2,4-D 10-50uM 및 키네틴 0.5uM을 첨가한 식물 조직 배양용 배지에서 멸균 알로에 베라 조직을 배양하여 알로에 베라의 캘러스를 유도하는 1단계; 및 유도된 캘러스를 알파-나프탈렌 초산 0.5uM과 키네틴 0.5uM을 첨가한 식물조직용 배지에서 배양하여 알로에 베라의 줄기와 뿌리를 형성시키는 2단계를 포함함을 특징으로 하는 알로에 베라의 증식방법.Incubating sterile aloe vera tissue in plant tissue culture medium to which alpha-naphthalene acetate or 2,4-D 10-50 uM and 0.5 kM of kinetin are added to induce aloe vera callus; And incubating the induced callus in a plant tissue medium containing 0.5 uM of alpha-naphthalene acetate and 0.5 uM of kinetin to form stems and roots of the aloe vera. 제1항에 있어서, 1단계에서 배지에 첨가되는 알파-나탈렌초산 또는 2,4-D의 양은 30uM이고 키네틴의 양은 5uM임을 특징으로 하는 알로에 베라의 증식방법.The method of propagating aloe vera according to claim 1, wherein the amount of alpha-natalene acetate or 2,4-D added to the medium in step 1 is 30 uM and the amount of kinetin is 5 uM. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940013237A 1994-06-13 1994-06-13 The tissue culture of aloe KR0133344B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940013237A KR0133344B1 (en) 1994-06-13 1994-06-13 The tissue culture of aloe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940013237A KR0133344B1 (en) 1994-06-13 1994-06-13 The tissue culture of aloe

Publications (2)

Publication Number Publication Date
KR960000012A true KR960000012A (en) 1996-01-25
KR0133344B1 KR0133344B1 (en) 1998-04-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940013237A KR0133344B1 (en) 1994-06-13 1994-06-13 The tissue culture of aloe

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101563351B1 (en) * 2015-04-23 2015-10-26 김권희 New aloe cultivar Aloe vera Saengjang and molecular marker for discriminating the same

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