KR950033482A - Immunoassay of Anti Treponema Palidium Antibodies - Google Patents
Immunoassay of Anti Treponema Palidium Antibodies Download PDFInfo
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- KR950033482A KR950033482A KR1019950004116A KR19950004116A KR950033482A KR 950033482 A KR950033482 A KR 950033482A KR 1019950004116 A KR1019950004116 A KR 1019950004116A KR 19950004116 A KR19950004116 A KR 19950004116A KR 950033482 A KR950033482 A KR 950033482A
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Abstract
본 발명은 감도가 향상된 항 트레포네마 팔리둠 항체를 분석하는 신규 분석 방법에 관한 것이다. 본 발명에 따르면, 혈청 중의 항 Tp 항체는 막 항원(15 및(또는) 17kDa)의 N-말단에 글루타티온-S-트란스페라제의 융합시 얻어지는 단백질인 G15 항원 및(또는) G17 항원과 시료 중의 항 Tp 항체 사이의 항원 항체 반응을 이용하는 효소 면역 분석법에 의해 분석된다.The present invention relates to a novel analytical method for analyzing anti-Treponema palidium antibodies with enhanced sensitivity. According to the present invention, the anti-Tp antibody in serum is a protein obtained from the fusion of glutathione-S-transferase to the N-terminus of the membrane antigen (15 and / or 17kDa) in the sample with the G15 antigen and / or G17 antigen. Analyzes are performed by enzymatic immunoassays using antigen antibody responses between anti-Tp antibodies.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 본 발명의 방법에서 사용하기 위한 G15 및 G17 항원의 구조의 도식도, 제2도는 47C4를 사용하는 비교 가능한 분석 결과와 비교하여, 항원으로서 G15 및 G17를 사용하는 본 발명의 방법에 따른 샌드위치 ELISA의 결과를 나타내는 그래프, 제3도는 천연 항원에 유사한 형태로서 이 콜리 발현 시스템에서 수득한 무 GST 성북 17항원을 사용하는 비교 가능한 분석 결과와 비교하여, 항원으로서 G17를 사용하는 본 발명의 방법에 따른 샌드위치 ELISA의 결과를 나타내는 그래프, 제4도는 Tp를 접촉한 고환으로부터 정제된 천연 47항원을 사용하는 분석 결과와 비교하여, 재조합 47C2항원을 사용하는 샌드위치 ELISA의 결과를 나타내는 그래프, 제5도는 본 발명의 방법에 따른 검사 혈청의 다양한 희석액을 사용하는 샌드위치 ELISA의 결과를 나타내는 그래프, 제6도는 3종의 재조합 47kDa 항원을 사용하는 검사 혈청의 다양한 희석액에 대한 샌드위치 ELISA의 결과를 나타내는 그래프, 제7도는 35종의 상이한 Tp양성 혈청에서, 본 발명의 방법에 따른 샌드위치 ELISA의 결과를 나타내는 그래프.1 is a schematic of the structure of the G15 and G17 antigens for use in the method of the invention, and FIG. 2 is a method of the invention using G15 and G17 as antigens, compared to comparable assay results using 47C 4 A graph showing the results of a sandwich ELISA according to FIG. 3 shows the use of G17 as an antigen, compared to comparable assay results using GST Seongbuk 17 Antigen obtained from this Coli expression system as a form similar to a native antigen. Graph showing the results of the sandwich ELISA according to the method, FIG. 4 is a graph showing the results of the sandwich ELISA using recombinant 47C 2 antigen, as compared to the results of analysis using the native 47 antigen purified from the testis in contact with Tp. 5 is a graph showing the results of sandwich ELISA using various dilutions of test serum according to the method of the present invention, and FIG. 6 is 3 types. A graph showing the results of a sandwich ELISA for various dilutions of test serum using recombinant 47 kDa antigen of FIG. 7 is a graph showing the results of a sandwich ELISA according to the method of the present invention in 35 different Tp positive sera.
Claims (3)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5467294 | 1994-02-28 | ||
JP94-54672 | 1994-02-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR950033482A true KR950033482A (en) | 1995-12-26 |
Family
ID=66548932
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1019950004116A KR950033482A (en) | 1994-02-28 | 1995-02-28 | Immunoassay of Anti Treponema Palidium Antibodies |
Country Status (1)
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KR (1) | KR950033482A (en) |
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1995
- 1995-02-28 KR KR1019950004116A patent/KR950033482A/en not_active Application Discontinuation
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