KR950031110A - Novel Monoclonal Antibodies Against Human Blood Apolipoprotein Non-100 (B-100) and Recombinant Hybridoma Cell Lines for Their Production - Google Patents

Novel Monoclonal Antibodies Against Human Blood Apolipoprotein Non-100 (B-100) and Recombinant Hybridoma Cell Lines for Their Production Download PDF

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KR950031110A
KR950031110A KR1019940012084A KR19940012084A KR950031110A KR 950031110 A KR950031110 A KR 950031110A KR 1019940012084 A KR1019940012084 A KR 1019940012084A KR 19940012084 A KR19940012084 A KR 19940012084A KR 950031110 A KR950031110 A KR 950031110A
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South Korea
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hybridoma cell
human blood
apolipoprotein
monoclonal antibodies
monoclonal antibody
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KR1019940012084A
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Korean (ko)
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KR0135708B1 (en
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곽주원
정태화
한문희
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김은영
한국과학기술연구원
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
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  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 인간 혈장 아포지단백질 B-100을 인식하는 신규단일클론 항체, 이들을 생산하는 재조합 하이브리도마 세포주 및 단일클론 항체를 이용한 혈장 아포지단백질 B-100의 농도를 측정하는 방법을 제공한다.The present invention provides a novel monoclonal antibody that recognizes human plasma apolipoprotein B-100, a recombinant hybridoma cell line that produces them, and a method for measuring the concentration of plasma apolipoprotein B-100 using monoclonal antibodies.

Description

인간 혈증 아포지단백질 비-100(B-100)에 대한 신규 단일클론 항체 및 이들의 생산을 위한 재조합 하이브리도마 세포주Novel Monoclonal Antibodies Against Human Blood Apolipoprotein Non-100 (B-100) and Recombinant Hybridoma Cell Lines for Their Production

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 융합반응전 아포지단백질 B-100으로 면역주사한 생쥐에서 채취한 혈액 샘플을 효소면역 분석법(ELISA)으로 분석한 결과를 나타내고, 제2도의 A는 HAT선택배지에서 재조합 하이브리도마 세포주의 배양 1일 후의 현미경 사진이다.FIG. 1 shows the results of analysis of blood samples taken from mice immunized with apolipoprotein B-100 before fusion reaction by enzyme-immunoassay (ELISA). It is a micrograph after 1 day of culture.

Claims (8)

인간 혈증 아포지단백질 B-100에 대해 특이적으로 반응하는 단일클론 항체.A monoclonal antibody that specifically responds to human blood apolipoprotein B-100. 제1항에 있어서, H-사슬이 IgG2b이고 L-사슬이 λ인 구조의 단일클론 항체 MabB9.The monoclonal antibody MabB9 of claim 1, wherein the H-chain is IgG2b and the L-chain is λ. 제1항에 있어서, H-사슬이 IgG2b이고 L-사슬이 λ인 구조의 단일클론 항체 MabB23.The monoclonal antibody MabB23 of claim 1, wherein the H-chain is IgG2b and the L-chain is λ. 인간 혈증 아포지단백질 B-100에 대해 특이적으로 반응하는 단일클론 항체를 생산하는 하아브리도마 세포주.A hybridoma cell line that produces monoclonal antibodies that specifically respond to human blood apolipoprotein B-100. 제4항에 있어서, 하이브리도마 세포주 H-MabB9(KCTC 0104 BP).The hybridoma cell line H-MabB9 (KCTC 0104 BP) according to claim 4. 제4항에 있어서, 하이브리도마 세포주 H-MabB23(KCTC 0105 BP).The hybridoma cell line H-MabB23 (KCTC 0105 BP) according to claim 4. 제4항 내지 6항 중 어느 한 항의 하이브리도마 세포주를 적당한 배지 또는 동물 체내에서 배양함을 포함하는, 인간 혈중 아포지단백질 B-100에 대해 특이적으로 반응하는 단일클론 항체의 제조방법.A method for producing a monoclonal antibody that specifically reacts against human blood apolipoprotein B-100, comprising culturing the hybridoma cell line of any one of claims 4 to 6 in a suitable medium or animal body. 제1항 내지 제3항중 어느 한 항의 단일클론 항체를 이용하여 혈장 아포지단백질 B-100을 포함한 지단백질의 농도를 측정하는 방법.A method for measuring the concentration of lipoproteins including plasma apolipoprotein B-100 using the monoclonal antibody of any one of claims 1 to 3. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940012084A 1994-05-31 1994-05-31 Novel mab against human blood apolipoprotein b-100 and KR0135708B1 (en)

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KR1019940012084A KR0135708B1 (en) 1994-05-31 1994-05-31 Novel mab against human blood apolipoprotein b-100 and

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KR1019940012084A KR0135708B1 (en) 1994-05-31 1994-05-31 Novel mab against human blood apolipoprotein b-100 and

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KR950031110A true KR950031110A (en) 1995-12-18
KR0135708B1 KR0135708B1 (en) 1998-04-23

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008038990A1 (en) * 2006-09-25 2008-04-03 Sj Biomed Inc. Anti-obese immunogenic hybrid polypeptides and anti-obese vaccine composition comprising the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008038990A1 (en) * 2006-09-25 2008-04-03 Sj Biomed Inc. Anti-obese immunogenic hybrid polypeptides and anti-obese vaccine composition comprising the same

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