KR950031109A - Method for preparing mixed venomous oil vaccine for swine epidemic diarrhea (RED) and infectious gastroenteritis (TGE) virus - Google Patents

Method for preparing mixed venomous oil vaccine for swine epidemic diarrhea (RED) and infectious gastroenteritis (TGE) virus Download PDF

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Publication number
KR950031109A
KR950031109A KR1019940010071A KR19940010071A KR950031109A KR 950031109 A KR950031109 A KR 950031109A KR 1019940010071 A KR1019940010071 A KR 1019940010071A KR 19940010071 A KR19940010071 A KR 19940010071A KR 950031109 A KR950031109 A KR 950031109A
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South Korea
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virus
vaccine
tge
piglets
diarrhea
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KR1019940010071A
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Korean (ko)
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KR0179947B1 (en
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윤인중
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윤인중
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/215—Coronaviridae, e.g. avian infectious bronchitis virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/215—Coronaviridae, e.g. avian infectious bronchitis virus
    • A61K39/225—Porcine transmissible gastroenteritis virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pulmonology (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

본 발명은 돼지 유행성 설사(Porcine Epidemic Diarrhea : PED)바이러스와 돼지 전염성 위장염(Transmissible Gastroenteritis : TEG)바이러스 감염으로 일어나는 자돈의 설사 및 폐사를 예방하기 위한 불활화 혼합 유성백신의 제조방법에 관한 것으로, 본 발명은 원숭이 콩팥 세포주(Vero cell)단층세포에 PED바이러스를 접종하고 트립신이 함유된 배지를 이용하여 고역가의 PDE바이러스를 증식시키고 여기에다 돼지 고환 세포주(ST cell)에 증식시킨 TGE바이러스를 혼합시킨 후 이들 바이러스의 항원성을 안전하게 보존시킬 수 있는 불활화제를 사용하여 바이러스를 비동화 시킨것이고, 또한 특수 유성 면역증강제를 혼합하여 백신의 면역원성을 증강케 하는 새로운 백신의 제조방법에 관한 것이며, 이와 같은 본 발명은 돼지 유행성 설사병(PED)바이러스와 전염성 위장염(TGE) 바이러스를 각각 증식시켜 채독한 다음 BEI으로 불활화 시키고 이들 두 바이러스를 혼합시킨 혼합 배양액에 특유의 유성면역증강제를 혼합하여 자돈 바이러스성 설사병을 예방함을 특징으로 하는 돼지 유행성 설사병 바이러스와 전염성 위장염 바이러스 혼합 사독 유성 백신의 제조방법이며, 이상과 같이 제조된 백신은 질병의 특성상 임신모돈의 분만 3-4주에 1회 접종하고 1차접종 2주후에 2차접종하여 분만 모돈에 고역가의 항체를 초유를 통해 자돈에 전달시키게 함으로 신생 자돈의 폐사를 막게함을 목적으로 한다.The present invention relates to a method for preparing an inactivated mixed oil vaccine to prevent diarrhea and death of piglets caused by Porcine Epidemic Diarrhea (PED) virus and Swine Transmissible Gastroenteritis (TEG) virus infection. The present invention inoculates the PED virus to monkey cell cell (Vero cell) monolayer cells and propagates the high titer of PDE virus using a medium containing trypsin, and then mixes the TGE virus grown on pig testicular cell line (ST cell). The present invention relates to a method of preparing a new vaccine which inactivates a virus by using an inactivating agent that can safely preserve the antigenicity of the virus, and also mixes a special oil-enhancing agent to enhance the immunogenicity of the vaccine. Invention of the swine epidemic diarrheal disease (PED) virus and infectious gastroenteritis (TGE) virus Swine epidemic diarrheal virus and infectious gastroenteritis virus mixture characterized by proliferation and extraction of each virus, inactivation with BEI, and mixing of the two viruses with a specific oily immune enhancer to prevent piglet viral diarrhea This is a method for the production of deadly oil-based vaccines. The vaccine prepared as described above is inoculated once every 3-4 weeks of gestational sows, and secondly inoculated 2 weeks after the first inoculation, so that high-potency antibodies are applied to the sows. It aims to prevent the death of new piglets by passing them through the piglets.

또한 이유 전후 자돈의 만성형 설사를 위해 이유전 잔돈의 백신 접종 또한 이용될 수 있다.Vaccination of pre-weaned change may also be used for chronic type diarrhea in piglets before and after weaning.

Description

돼지 유행성 설사병(RED)과 전염성 위장염(TGE)바이러스 혼합사독 유성백신의 제조방법Method for preparing mixed venomous oil vaccine for swine epidemic diarrhea (RED) and infectious gastroenteritis (TGE) virus

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (1)

돼지 유행성 설사병(PED)바이러스와 전염성 위장염(TGE) 바이러스를 각각 증식시켜 채독한 다음 BEI으로 불활화 시키고 이들 두 바이러스를 혼합시킨 혼합 배양액에 특유의 유성면역증강제를 혼합하여 자돈 바이러스성 설사병을 예방함을 특징으로 하는 돼지 유행성 설사병 바이러스와 전염성 위장염 바이러스 혼합 사독 유성 백신의 제조방법.The pig epidemic diarrheal disease (PED) virus and infectious gastroenteritis (TGE) virus are multiplied and harvested, inactivated by BEI, and mixed with these two viruses in a mixed culture medium to prevent piglets viral diarrhea. Method for producing a swine pandemic diarrheal virus and infectious gastroenteritis virus mixed deadly oil vaccine. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940010071A 1994-05-06 1994-05-06 Vaccine for porcine epidemic diarrhea virus and transmissible gastroenteritis virus KR0179947B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940010071A KR0179947B1 (en) 1994-05-06 1994-05-06 Vaccine for porcine epidemic diarrhea virus and transmissible gastroenteritis virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940010071A KR0179947B1 (en) 1994-05-06 1994-05-06 Vaccine for porcine epidemic diarrhea virus and transmissible gastroenteritis virus

Publications (2)

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KR950031109A true KR950031109A (en) 1995-12-18
KR0179947B1 KR0179947B1 (en) 1999-03-20

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100622399B1 (en) * 2004-01-14 2006-09-18 대한민국 Vaccine Comprising the Mixture of Inactivated TGEV and PEDV, and Preparation Method thereof
KR101430224B1 (en) * 2012-03-06 2014-10-15 농업회사법인 제이비솔루션 주식회사 Medium for proliferating porcine epidemic diarrhoea virus and method for proliferating porcine epidemic diarrhoea virus
JP6395855B2 (en) 2014-04-03 2018-09-26 ベーリンガー インゲルハイム フェトメディカ インコーポレイテッド Porcine epidemic diarrhea virus vaccine

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