KR950023730A - Hermaphroditism Method and DNA Molecules Used in the Method - Google Patents

Hermaphroditism Method and DNA Molecules Used in the Method Download PDF

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KR950023730A
KR950023730A KR1019940000715A KR19940000715A KR950023730A KR 950023730 A KR950023730 A KR 950023730A KR 1019940000715 A KR1019940000715 A KR 1019940000715A KR 19940000715 A KR19940000715 A KR 19940000715A KR 950023730 A KR950023730 A KR 950023730A
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South Korea
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nucleotide sequence
seq
specific dna
bovine
sequence represented
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KR1019940000715A
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Korean (ko)
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도시유끼 구도
요시아끼 이따가끼
세이지 사또
시즈야 스또
도요오 나까무라
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원본미기재
이또햄 가부시끼가이샤
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Priority to KR1019940000715A priority Critical patent/KR950023730A/en
Publication of KR950023730A publication Critical patent/KR950023730A/en

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Abstract

하이브리다이즈법을 사용한 소의 자웅의 판별방법 및 이 판별방법에 사용하는 프로브로써 사용한 DNA분자의 제공 및 PCR 법을 사용한 소의 자웅을 용이하게 판별 가능한 방법 및 이 방법에 사용하는 PCR프라이머의 제공하는 것을 목적으로 하고 숫소의 게놈에 특이적으로 하이브리다이즈 하는 DNA, 자웅쌍방의 소의 게놈에 하이브리다이즈하는 DNA 및 이들의 DNA유래의 프라이머를 사용하여 PCR법에 의해서 특정 유전자를 증폭시켜서 이에 의해서 얻어지는 PCR산물의 종류를 특정하여 식별하는 것을 특징으로 하는 소배의 자웅식별방법.The method of discriminating the bovine herb using the hybridization method and the method of providing the DNA molecules used as the probes used in this determination method and the method of easily determining the bovine herb using the PCR method and providing the PCR primers used in the method PCR obtained by amplifying a specific gene by PCR using a target DNA that hybridizes specifically to a male genome, a DNA hybridized to a male genome of both sexes, and primers derived from these DNAs The male identification method of Sobae, characterized by identifying the type of product.

Description

소의 자웅판별 방법 및 이 방법에 사용하는 DNA분자Hermaphroditism Method and DNA Molecules Used in the Method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 웅특이적 DNA단편을 클로닝하는 방법의 개략도,1 is a schematic of a method for cloning a male specific DNA fragment,

제2도는 웅 DNA를 특이적으로 식별하는 클론을 단리한 전기영동사진,2 shows electrophoresis images of clones that specifically identify male DNA.

제3도는 웅특이적으로 회합하는 2차적 클론에 대한 전기영동사진.FIG. 3 shows electrophoresis images of secondary clones that associate specifically.

Claims (14)

배열번호 1로 나타낸 염기배열로 표시된 염기배열을 포함하는 수소 Y염색체 특이적 DNA분자.A hydrogen Y chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence represented by SEQ ID NO: 1. 배열번호 2로 나타낸 염기배열로 표시되는 염기배열을 포함하는 숫소 Y염색체 특이적 DNA분자.Ox Y chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence shown in SEQ ID NO: 2. 배열번호 3로 나타낸 염기배열로 표시되는 염기배열을 포함하는 숫소 Y염색체 특이적 DNA분자.A male Y chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence represented by SEQ ID NO: 3. 배열번호 4로 나타낸 염기배열로 표시되는 염기배열을 포함하는 수소 Y염색체 특이적 DNA분자.A hydrogen Y chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence shown in SEQ ID NO: 4. 배열번호 1, 배열번호 2, 배열번호 3 또는 배열번호 4로 나타낸 염기배열로 표시되는 염기배열과 실질적으로 동등한 염기배열을 포함하는 수소 Y염색체 특이적 DNA분자.A hydrogen Y chromosome specific DNA molecule comprising a nucleotide sequence substantially equivalent to a nucleotide sequence represented by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4. 배열번호 5로 나타낸 염기배열로 표시되는 염기배열을 포함하는 소 염색체 특이적 DNA분자.A bovine chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence shown in SEQ ID NO: 5. 배열번호 6로 나타낸 염기배열로 표시되는 염기배열을 포함하는 소 염색체 특이적 DNA분자.A bovine chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence shown in SEQ ID NO: 6. 배열번호 7로 나타낸 염기배열로 표시되는 염기배열을 포함하는 소 염색체 특이적 DNA분자.A bovine chromosome specific DNA molecule comprising a nucleotide sequence represented by the nucleotide sequence shown in SEQ ID NO: 7. 배열번호 5, 배열번호 6, 배열번호 7로 나타낸 염기배열과 실질적으로 동등한 염기배열을 포함하는 소 염색체 특이적 DNA분자.A small chromosome specific DNA molecule comprising a nucleotide sequence substantially equivalent to the nucleotide sequence shown in SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7. 제1∼5항의 기재의 숫소 Y염색체 특이적 DNA분자의 일부 또는 그들의 상보쇄의 일부를 포함하고 염기길이가 15염기 이상인 숫소 Y염색체에 특이적인 유전자 증폭용 DNA프라이머.A DNA primer for gene amplification, which comprises a part of the male Y chromosome specific DNA molecules according to claims 1 to 5 or a part of their complementary chains, and which has a base length of 15 bases or more. 제6∼8항 기재의 소 염색체 특이적 DNA분자의 일부 또는 그들의 상보쇄의 일부를 포함하고 염기길이가 15염기 이상인 소염색체에 특이적인 유전자 증폭용 DNA프라이머.A DNA primer for gene amplification, which comprises a part of the small chromosome-specific DNA molecules according to claims 6 to 8 or a part of their complementary chains, and which is specific for the small chromosome having a base length of 15 bases or more. 식별용 표지를 부여한 제1항∼제5항 기재의 숫소 Y염색체 특이적 DNA분자를 식별용 프로브로서 소의 DNA에 하이브리다이즈시키고 이어서 상기 소의 DNA에 하이브리다이즈시킨 상기 식별용 프로브를 검출함을 특징으로 하는 소의 자웅판별 방법.The identification probe labeled with the Y Y chromosome specific DNA molecule according to Claims 1 to 5 to which the identification label was identified was hybridized to bovine DNA as an identification probe, and then hybridized to the bovine DNA. Characteristic way of judging cow. 제10항 기재 유전자 증폭용 DNA프라이머를 PCR프라이머로 사용하여 PCR법에 의해서 소의 DNA를 증폭시켜 이 PCR산물을 특정함을 특징으로 하는 소의 자웅 판별방법.A method for discriminating a bovine herb, characterized by amplifying bovine DNA by a PCR method using a DNA primer for amplifying the gene of claim 10 as a PCR primer. 제13항에 있어서, 제10항 및 제11항 기재의 유전자 증폭용 DNA프라이머를 동시에 PCR프라이머로 사용함을 특징으로 하는 소의 자웅판별방법.The method of claim 13, wherein the DNA amplification DNA primers of claim 10 and 11 are used simultaneously as a PCR primer. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940000715A 1994-01-17 1994-01-17 Hermaphroditism Method and DNA Molecules Used in the Method KR950023730A (en)

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