KR950023723A - Enzymatic Hydrolysis Method of Starch-Based Substances - Google Patents

Enzymatic Hydrolysis Method of Starch-Based Substances Download PDF

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Publication number
KR950023723A
KR950023723A KR1019940000207A KR19940000207A KR950023723A KR 950023723 A KR950023723 A KR 950023723A KR 1019940000207 A KR1019940000207 A KR 1019940000207A KR 19940000207 A KR19940000207 A KR 19940000207A KR 950023723 A KR950023723 A KR 950023723A
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KR
South Korea
Prior art keywords
starch
fiber
hydrolysis method
cellobiase
enzymatic hydrolysis
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Application number
KR1019940000207A
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Korean (ko)
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KR0135543B1 (en
Inventor
최차용
이용희
양영렬
이승준
안용호
Original Assignee
안덕기
삼성엔지니어링 주식회사
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Priority to KR1019940000207A priority Critical patent/KR0135543B1/en
Publication of KR950023723A publication Critical patent/KR950023723A/en
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Publication of KR0135543B1 publication Critical patent/KR0135543B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Abstract

본 발명은 카사바, 감자, 고구마와 같은 섬유질 함유 전분질계 물질을 효소적 가수분해방법에 관한 것이다. 종래, 전분을 포도당으로 가수분해함에 있어서, 글루코아밀라제계 효소를 사용하였으나 전분주위의 섬유질에 의해 그구코아밀라제와 전분의 접촉이 방해되어 반응시간이 길고 잔류 섬유질에 의한 잔류고형분의 처리에 문제가 있었다.The present invention relates to a method for enzymatic hydrolysis of fiber-containing starch materials such as cassava, potatoes, and sweet potatoes. Conventionally, in the hydrolysis of starch to glucose, glucoamylase-based enzymes were used, but the contact between starch-glucose and starch was hindered by the fiber around the starch, resulting in a long reaction time and problems in the treatment of residual solids by residual fibers. .

본 발명은 pH4.0-4.0, 반응온도 60℃에서 글루코아밀라제와 함께 셀루라제와 셀로바이아제를 첨가하여 가수분해하는 것으로 섬유질이 셀룰라제와 셀로바이아제에 의해 분해되어 결과적으로 전분의 당화 반응시간이 단축되고 잔류 섬유질을 최소화하며 당화효율을 증대되는 효과가 있다.The present invention hydrolyzes by adding cellulase and cellobiase together with glucoamylase at pH 4.0-4.0 and a reaction temperature of 60 ° C. The fiber is degraded by cellulase and cellobiase, and consequently the starch saccharification reaction time. This has the effect of shortening and minimizing residual fiber and increasing glycosylation efficiency.

Description

전분질계 물질의 효소적 가수분해 방법Enzymatic Hydrolysis Method of Starch-Based Substances

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (3)

섬유질을 함유하는 카사바, 감자, 고무마등의 전분질계 물질을 글루코아밀라제계 효소를 이용하여 발효가능한 포도당으로 가수분해하는 방법에 있어서, 셀룰라제와 셀로바이아제를 첨가하는 것을 특징으로 하는 가수분해 방법.A method of hydrolyzing starch-based substances such as cassava, potato, and rubber yam containing fibers into glucose which can be fermented using a glucoamylase-based enzyme, wherein the hydrolysis method comprises adding cellulase and cellobiase. 제1항에 있어서, pH4.0-5.0, 반응온도 60℃에서 수행하는 것을 특징으로 하는 가수분해방법.The hydrolysis method according to claim 1, wherein the hydrolysis method is performed at pH 4.0-5.0 and a reaction temperature of 60 ° C. 제1항 또는 2항에 있어서, 글로코아밀라제는 전분질계 물질의 전분 함유량의 0.15%이상, 셀룰라제와 셀로바이아제는 각각 섬유질 함유량의 0.2-1.0%, 0.4-0.2%첨가하는 것을 특징으로 하는 가수분해방법.The method according to claim 1 or 2, wherein the glycoamylase is 0.15% or more of the starch content of the starch-based material, and the cellulase and the cellobiase add 0.2-1.0% and 0.4-0.2% of the fiber content, respectively. Hydrolysis method. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940000207A 1994-01-07 1994-01-07 Enzymatic hydrolysis of starch derivatives KR0135543B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940000207A KR0135543B1 (en) 1994-01-07 1994-01-07 Enzymatic hydrolysis of starch derivatives

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940000207A KR0135543B1 (en) 1994-01-07 1994-01-07 Enzymatic hydrolysis of starch derivatives

Publications (2)

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KR950023723A true KR950023723A (en) 1995-08-18
KR0135543B1 KR0135543B1 (en) 1998-04-23

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KR20020096432A (en) * 2001-06-19 2002-12-31 윤현희 A Process for Lactic Acid Production

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