KR950018484A - Korean Cattle Breed Discrimination by Analysis of DNA Polymorphism - Google Patents

Korean Cattle Breed Discrimination by Analysis of DNA Polymorphism Download PDF

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KR950018484A
KR950018484A KR1019930030237A KR930030237A KR950018484A KR 950018484 A KR950018484 A KR 950018484A KR 1019930030237 A KR1019930030237 A KR 1019930030237A KR 930030237 A KR930030237 A KR 930030237A KR 950018484 A KR950018484 A KR 950018484A
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dna
cow
primer
imported
dna fragment
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KR1019930030237A
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KR970007377B1 (en
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박용환
류진창
정태영
유영복
정미정
김범기
이창수
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이판석
농촌진흥청
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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Abstract

본 발명은 한우 품종을 수입 소 또는 젖소의 품종으로부터 판별하기 위한 DNA 다형성 분석법에 사용하기 위한 DNA 단편 및 이 단편을 이용하여 한우 품종을 수입 소 또는 젖소의 품종으로부터 판별하는 방법을 제공한다.The present invention provides a DNA fragment for use in a DNA polymorphism analysis method for discriminating a Hanwoo cultivar from a breed of an imported cow or a cow, and a method for discriminating a Hanwoo cultivar from a breed of an imported cow or a cow using the fragment.

본 발명에 의해 제공되는 프라이머는 염기 배열 AAGGTCCTAGGGTCCTGGG의 전부 혹은 일부를 포함하거나 또는 이 배열과 균등한 DNA 단편이며; 본 발명에 따른 한우 품종의 판별방법은 상기 프라이머를 피험 소의 DNA 와 접촉시키고, 상기 프라이머가 부착된 피험 소의 DNA 단편을 검출해냄을 특징으로 한다. 이 판별방법에서는 프라이머 또는 프로브 DNA 단편과 피험 소의 DNA 단편을 41℃이하의 온도에서 30초-100초간 접촉 시키며, 피험 소의 DNA 는 혈액 또는 고기 또는 기타 다른 부위로부터 채취할 수 있다. 이 판별방법에 의해서 프라이머 단편과 부착된 DNA 단편을 갖는 것으로 판명된 소는 한우 품종이 아닌 젖소 또는 수입 소 품종이다.The primers provided by the present invention are DNA fragments which include or are equivalent to all or part of the base sequence AAGGTCCTAGGGTCCTGGG; The method for discriminating a Korean cattle breed according to the present invention is characterized in that the primer is contacted with a DNA of a test subject, and the DNA fragment of the test subject with the primer attached thereto is detected. In this method, the primer or probe DNA fragment and the test DNA fragment are contacted for 30 seconds to 100 seconds at a temperature of 41 ° C. or lower, and the test DNA may be taken from blood or meat or other parts. Cows found to have primer fragments and attached DNA fragments by this discrimination method are not cow cattle but cow or imported cow varieties.

따라서, 본 발명의 방법에 의하면 신속하고 간단하게, 그리고 정확하게 한우 또는 한우 고기를 젖소 또는 수입 소 또는 그의 고기로부터 판별해낼 수 있다.Therefore, according to the method of the present invention, it is possible to quickly and simply and accurately discriminate Korean beef or Korean beef from cows or imported cows or their meats.

Description

디엔에이(DNA) 다형성의 분석에 의한 한우 품종 판별법Korean Cattle Breed Discrimination by Analysis of DNA Polymorphism

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 한우와 젖소 품종에서의 HWB의 검출여부를 보여주는 전기영동 사진이다.FIG. 1 is an electrophoretic photograph showing the detection of HWB in Korean cattle and cow varieties.

제2도는 소는 제놈 DNA 양이 HWB의 검출각도에 미치는 영향을 보여주는 전기영동사진이다.2 is an electrophoretic image showing the effect of the amount of genomic DNA on the detection angle of HWB.

제3도는 RAPD법의 프라이머 부착시간이 HWB의 검출각도에 미치는 영향을 보여주는 전기영동 사진이다.Figure 3 is an electrophoresis picture showing the effect of the primer attachment time of the RAPD method on the detection angle of HWB.

제4도는 RAPD법의 프라이머 부착온도가 HWB의 검출각도에 미치는 영향을 보여주는 전기영동 사진이다.4 is an electrophoretic photograph showing the effect of the primer adhesion temperature of the RAPD method on the detection angle of HWB.

Claims (6)

염기 배열 AAGGTCCTAGGGTCCTGGG의 전부 혹은 일부를 포함하거나 또는 이 배열과 균등한 DNA 단편.DNA fragments that contain, or are equivalent to, all or part of the base sequence AAGGTCCTAGGGTCCTGGG. 염기 배열 AAGGTCCTAGGGTCCTGGG의 전부 혹은 일부를 포함하는 DNA 단편을 프라이머 또는 프로브로 이용하여 이를 피험 소의 DNA 와 접촉시키고, 상기 프라이머가 부착된 피험 소의 DNA 단편을 검출해냄을 특징으로 하는 한우 또는 한우 고기를 젖소 또는 수입 소 또는 그의 고기와 구별하는 방법.A DNA fragment comprising all or a portion of the nucleotide sequence AAGGTCCTAGGGTCCTGGG is used as a primer or a probe to be contacted with a DNA of a test subject, and the DNA fragment of the test subject to which the primer is attached is detected. How to distinguish from imported cattle or their meat. 제2항에 있어서, 프라이머 또는 프로브 DNA 단편과 피험 소의 DNA 단편을 41℃이하의 온도에서 30초-100초간 접촉시킴을 특징으로 하는 방법.The method of claim 2, wherein the primer or probe DNA fragment and the test DNA fragment are contacted for 30 seconds to 100 seconds at a temperature of 41 ° C. or less. 제2항에 있어서, 피험 소의 공급원은 혈액, 고기, 털 또는 발톱 등임을 특징으로 하는 방법.The method of claim 2, wherein the source of the test subject is blood, meat, hair or claws, or the like. 제4항에 있어서, 혈액을 공급원으로 사용하는 경우 피험 소의 DNA양은 10-30ng임을 특징으로 하는 방법.The method of claim 4, wherein the DNA amount of the test subject is 10-30 ng when blood is used as the source. 제4항에 있어서, 고기를 공급원으로 사용하는 경우 피험 소의 DNA양은 10-30ng임을 특징으로 하는 방법.The method of claim 4, wherein the DNA amount of the test subject is 10-30 ng when meat is used as a source. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930030237A 1993-12-28 1993-12-28 Distinction method for korean cattle by dna polymorphism KR970007377B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100933223B1 (en) * 2006-11-14 2009-12-22 대한민국 SNP marker combination for Korean beef

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100933223B1 (en) * 2006-11-14 2009-12-22 대한민국 SNP marker combination for Korean beef

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