KR950017921A - Hydrolysis Method of Dipeptide Compounds - Google Patents

Hydrolysis Method of Dipeptide Compounds Download PDF

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Publication number
KR950017921A
KR950017921A KR1019930027941A KR930027941A KR950017921A KR 950017921 A KR950017921 A KR 950017921A KR 1019930027941 A KR1019930027941 A KR 1019930027941A KR 930027941 A KR930027941 A KR 930027941A KR 950017921 A KR950017921 A KR 950017921A
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South Korea
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acid
phenylalanine
aspartyl
methylester
water
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KR1019930027941A
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Korean (ko)
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KR970011451B1 (en
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최경석
현일
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유영학
주식회사 미 원
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Publication of KR950017921A publication Critical patent/KR950017921A/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C227/14—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof
    • C07C227/18—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof by reactions involving amino or carboxyl groups, e.g. hydrolysis of esters or amides, by formation of halides, salts or esters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/34—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton containing six-membered aromatic rings
    • C07C229/36—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton containing six-membered aromatic rings with at least one amino group and one carboxyl group bound to the same carbon atom of the carbon skeleton

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 L-페닐알라닌 메틸에스테르와 N-포르밀-L-아스파틸 무수물의 반응에 있어, α아스파탐을 제조하는 공정에서 부산물로 생성되는 3-벤질-6-카르복시메틸-2,5-디케토피레라진, α-아스파틸-L-페닐알라닌, β-아스파틸-L-페닐알라닌, β-아스파틸 페닐알라닌 메틸에스테르, α-아스파틸-β-메틸에스테르-L-페닐알라닌 메틸에스테르, β-아스파틸-α-메틸에스테르-L-페닐알라닌 에틸에스테르를 가수분해하여 L-페닐알라닌과 L-아스파트산을 제조하는 방법을 제공한다.The present invention relates to 3-benzyl-6-carboxymethyl-2,5-diketo produced as a by-product in the process for producing α aspartame in the reaction of L-phenylalanine methyl ester with N-formyl-L-aspartyl anhydride. Pyrazine, α-aspartyl-L-phenylalanine, β-aspartyl-L-phenylalanine, β-aspartyl phenylalanine methylester, α-aspartyl-β-methylester-L-phenylalanine methylester, β-aspartyl- A method of producing L-phenylalanine and L-aspartic acid by hydrolyzing α-methylester-L-phenylalanine ethyl ester is provided.

본 발명의 방법은 물, 물 부피에 대하여 3-20%(V/V)의 탄소수 1 내지 4의 저급 알콜, 물, 부피에 대하여 1-15%(V/V)의 유기산, 및 물 부피에 대하여 5-20(W/V)의 강산으로 이루어진 계에서 반응온도 70-130℃, 반응시간 1-5시간의 조건하에 실시함을 특징으로 하며, 유기산으로는 개미산 및 초산의 혼합물이 사용되고, 강산으로는 염산, 황산, 인산 또는 할로겐산이 사용된다.The process of the present invention is directed to water, 3-20% (V / V) lower alcohols having 1 to 4 carbon atoms, water, 1-15% (V / V) organic acids by volume, and water volume. It is characterized in that it is carried out under the conditions of reaction temperature of 70-130 ℃, reaction time of 1-5 hours in a system consisting of 5-20 (W / V) strong acid, and a mixture of formic acid and acetic acid is used as the organic acid, Hydrochloric acid, sulfuric acid, phosphoric acid or halogen acid are used as the reactor.

본 발명의 방법에 의하여 고순도 및 고수율로 L-페닐알라닌과 L-아스파트산을 제조할 수 있다.L-phenylalanine and L-aspartic acid can be prepared in high purity and high yield by the method of the present invention.

Description

디펩티드 화합물의 가수분해 방법Hydrolysis Method of Dipeptide Compounds

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (3)

3-벤질-6-카르복시메틸-2.5-디케토피페라진, α-아스파틸-L-페닐알라닌, β-아스파틸-L-페닐알라닌, β-아스파틸 페닐알라닌 메틸에스테르, α-아스파틸-β-메틸에스테르-L-페닐알라닌 메틸에스테르 및 β-아스파틸-α-메틸에스테르-L-페닐알라닌 메틸에스테르로 이루어진 군에서 선택된 1종 또는 2종 이상의 디펩티드 화합물의 혼합물을 가수분해하여 L-페닐알라닌과 L-아스파트산을 제조하는 방법에 있어서, 가tn분해반응을 물, 물 부피에 대하여 3-20%(Ⅴ/Ⅴ)의 탄소수 1 내지 4의 저급 알콜, 물 부피에 대하여 1-15%(Ⅴ/Ⅴ)의 유기산, 및 물 부피에 대하여 5-20%(W/Ⅴ)의 강산으로 이루어진 계에서 반응온도 70-130℃, 반응시간 1-5시간의 조건하에 실시함을 특징으로 하는 방법.3-benzyl-6-carboxymethyl-2.5-diketopiperazine, α-aspartyl-L-phenylalanine, β-aspartyl-L-phenylalanine, β-aspartyl phenylalanine methylester, α-aspartyl-β-methyl L-phenylalanine and L-as hydrolyzed by hydrolyzing a mixture of one or more dipeptide compounds selected from the group consisting of ester-L-phenylalanine methylester and β-aspartyl-α-methylester-L-phenylalanine methylester In the process for preparing formic acid, the tn decomposition reaction is carried out by water, 3-20% (V / V) lower alcohols having 1 to 4 carbon atoms, and 1-15% (V / V) relative to the volume of water. ) And a strong acid of 5-20% (W / V) relative to the volume of water, and the reaction is carried out under the conditions of a reaction temperature of 70-130 ° C. and a reaction time of 1-5 hours. 제1항에 있어서, 유기산은 개미산 및 초산의 혼합물이며, 개미산은 1-10%(Ⅴ/Ⅴ), 초산은 1-5%(Ⅴ/Ⅴ)의 양으로 사용됨을 특징으로 하는 방법.The method of claim 1, wherein the organic acid is a mixture of formic acid and acetic acid, formic acid is used in an amount of 1-10% (V / V), and acetic acid is 1-5% (V / V). 제1항에 있어서, 강산은 염산, 황산, 인산 또는 할로겐산에서 선택된 것임을 특징으로 하는 방법.The method of claim 1 wherein the strong acid is selected from hydrochloric acid, sulfuric acid, phosphoric acid or halogen acid. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930027941A 1993-12-16 1993-12-16 Method of hydrolysis of dipeptide KR970011451B1 (en)

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KR950017921A true KR950017921A (en) 1995-07-20
KR970011451B1 KR970011451B1 (en) 1997-07-11

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