KR950014302A - Cell Wall Attached Polysaccharides Produced by Zooglia sp. - Google Patents

Cell Wall Attached Polysaccharides Produced by Zooglia sp. Download PDF

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KR950014302A
KR950014302A KR1019930025571A KR930025571A KR950014302A KR 950014302 A KR950014302 A KR 950014302A KR 1019930025571 A KR1019930025571 A KR 1019930025571A KR 930025571 A KR930025571 A KR 930025571A KR 950014302 A KR950014302 A KR 950014302A
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polysaccharides
cell wall
produced
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KR1019930025571A
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KR970006160B1 (en
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공재열
공인수
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공재열
공인수
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 해양으로부터 분리한 미생물인 쥬글리아 스페시스(Zoogloea sp.)(한국종균협회 KCCM-10036. 기탁일자 1993년 5월 15일)로부터 세포외로 생산된 다당에 관한 것이다.The present invention relates to a polysaccharide produced extracellularly from the microorganism isolated from the ocean, joogloea sp. (Korean spawn association KCCM-10036. Date of deposit 15 May 1993).

일반적으로 쥬글리아 속 미생물이 생산하는 다당류는 현재까지 모두 한가지 종류만을 생산하고 있는데 생산된 다당류의 조성은 글루코스(Glucose), 갈락토스(Galaclose), 피루빅산(Phyruric)등으로 구성되어 있다. 또한 분리한 쥬글리아 속 미생물은 모두 육상에서 분리된 것뿐이다.In general, the polysaccharides produced by the microorganisms of the genus Zuglia have produced only one kind to date. The composition of the produced polysaccharides is composed of glucose, galactose, pyruvic acid, and the like. In addition, all the isolated microorganisms of the genus Juglia are separated from the land.

그러나 본 발명에서 사용된 쥬글리아 속 미생물은 바다속에 존재하는 해조류로부터 분리하였을뿐만 아니라 다당류를 생산하고 있으며 다당의 종류도 현재까지 알려진 다당과는 전혀 그 조성을 달리하고 있다.However, the microorganism of the genus Juglia used in the present invention is not only isolated from seaweeds present in the sea, but also produces polysaccharides, and the types of polysaccharides differ from those of polysaccharides known to date.

Description

쥬글리아(Zoogloea sp.)가 생산하는 세포벽 부착 다당Cell Wall Attached Polysaccharides Produced by Zooglia sp.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (1)

배지의 조성은 Peptene 5g, Glucose 10g, Na2HPO40.008g, NH4NO30.0016g, NaCl 23.4g, KCl 0.7g, MgCl2ㆍ6H2O 10.6g, CaCl21.1g, Na2SO43.9g, NaHCO30.2g, (NH4)2SO41.0g, K2HPO40.01g, Tris 6.05g, D.W.1ℓ. pH 7.8을 사용하여 미생물을 30℃에서 72시간 진탕배양시킨 후 배양액을 3~5℃에서 30,000~35,000 x g에서 10분간 원심분리하여 상층액과 침전물(미생물세포)로 분리한 미생물세포의 침전물에 동량의 증류수를 가한 다음 50~60℃에서 1~3시간 동안 강하게 교반시키면서 가열처리한 다음 35~45℃에서 30,000~35,000 x g에서 8~12분간 원심 분리하여 세포로부터 세포벽 부착 다당을 분리하여 최종 농도가 8~12%(Weight/Volume)가 되도록 세틸피리디니움 클로라이드(CPC, Cetylpyridinium Chloride)를 가하여 산성다당(Acidic poly-Saccharide)을 분리한 다음 이를 다시 10%의 염화나트륨(NaCl)용액에 완전히 재용시킨 후 2배의 냉각된 아세톤(Cold Acetone)으로 침전시켜 투석, 동결건조하여 분리한 것을 인산 완충용액(Phosphate Buffer)에 용해시킨 후 Sepharose CL-4B가 채워진 컬럼(Column)에서 켈크로마토그래피(Gelehromatography)를 행하여 완전정제하여서 되는 쥬글리아(Zoogloea sp.)가 생산하는 세포벽 부착 다당.The composition of the medium was Peptene 5g, Glucose 10g, Na 2 HPO 4 0.008g, NH 4 NO 3 0.0016g, NaCl 23.4g, KCl 0.7g, MgCl 2 6H 2 O 10.6g, CaCl 2 1.1g, Na 2 SO 4 3.9 g, 0.2 g NaHCO 3 , 1.0 g (NH 4 ) 2 SO 4 , 0.01 g K 2 HPO 4 , 6.05 g Tris, DW1 L. After shaking the microorganism with pH 7.8 for 72 hours at 30 ° C, the culture medium was centrifuged at 30,000 ~ 35,000 xg for 10 minutes at 3 ~ 5 ° C, and then the same amount as the supernatant and precipitate (microbial cell). After distilled water was added, the mixture was heated with vigorous stirring for 1 to 3 hours at 50 to 60 ° C, and then centrifuged at 30,000 to 35,000 xg at 35 to 45 ° C for 8 to 12 minutes to separate cell wall-attached polysaccharides from cells. Cetylpyridinium chloride (CPC, Cetylpyridinium Chloride) was added to obtain 8-12% (Weight / Volume). It was then precipitated with two times of cold acetone (Cold Acetone), dialyzed and lyophilized, and then dissolved in phosphate buffer (Phosphate Buffer), followed by kel chromatography (Geleh) on a column filled with Sepharose CL-4B. Cell wall-attached polysaccharides produced by Joogloea sp., which is purified by romatography. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930025571A 1993-11-25 1993-11-25 Processing method of polysaccharide produced from zoogloea sp KR970006160B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930025571A KR970006160B1 (en) 1993-11-25 1993-11-25 Processing method of polysaccharide produced from zoogloea sp

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KR950014302A true KR950014302A (en) 1995-06-15
KR970006160B1 KR970006160B1 (en) 1997-04-24

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