KR950011465A - Method for Purifying Proteins Using Antibodies - Google Patents

Method for Purifying Proteins Using Antibodies Download PDF

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Publication number
KR950011465A
KR950011465A KR1019930020673A KR930020673A KR950011465A KR 950011465 A KR950011465 A KR 950011465A KR 1019930020673 A KR1019930020673 A KR 1019930020673A KR 930020673 A KR930020673 A KR 930020673A KR 950011465 A KR950011465 A KR 950011465A
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KR
South Korea
Prior art keywords
protein
earthworm
antibody
present
purifying
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Application number
KR1019930020673A
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Korean (ko)
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KR970002491B1 (en
Inventor
문상범
이성희
고형곤
Original Assignee
김정순
제일제당 주식회사
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Priority to KR1019930020673A priority Critical patent/KR970002491B1/en
Publication of KR950011465A publication Critical patent/KR950011465A/en
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Publication of KR970002491B1 publication Critical patent/KR970002491B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 지렁이로부터 혈전 분해능을 갖는 단백질을 그 항체를 사용하여 정제하는 방법에 관한 것이다.The present invention relates to a method for purifying a protein having thrombus resolution from an earthworm using the antibody.

본 발명에 따른 항체를 이용한 단백질의 정제방법은 먼지 지렁이로부터 순수 분리한 혈정 분해능을 갖는 단백질을 토끼에 주사하여 면역화시켜 그 단백질에 대한 항체를 만들고, 면역화된 토끼의 혈액에서 분리한 항체를 사용하여 면역 친화성 수지를 제조하며, 이를 사용하여 지렁이 추출액으로부터 혈전분해능을 갖는 단백질을 순수 분리함을 특징으로 한다.Protein purification method using the antibody according to the present invention by immunizing rabbits by injecting a protein having a blood serum resolution purely isolated from the dust earthworm to make an antibody to the protein, using the antibody isolated from the blood of the immunized rabbit Immune affinity resins are prepared and characterized in that they are used to purely separate proteins having thrombolytic activity from the earthworm extract.

본 발명에 따른 항체를 이용한 단백질의 정제방법에 의하면 지렁이로부터 혈전분해능 단백질을 높은 순도로 용이하게 분리해낼 수 있다.According to the method for purifying protein using the antibody according to the present invention, it is possible to easily separate the thrombolytic protein from the earthworm with high purity.

Description

항체를 이용한 단백질의 정제방법Method for Purifying Proteins Using Antibodies

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 프로테인 A세파로즈 칼럼 크로마토그래피를 이용하여 혈전 분해능을 갖는 단백질에 대한 항체를 정제하는 프로파일.1 is a profile for purifying antibodies to proteins having thrombus resolution using protein A Sepharose column chromatography.

Claims (2)

지렁이의 혈전분해능을 갖는 단백질에 대한 항체를 CNBr 활성화 세파로즈에 결합시켜 제조된 면역 친화성 수지컬럼을 이용하여 지렁이 추출물로부터 혈전 분해능을 갖는 단백질을 순수 분리하는 방법.A method of purely separating a protein having thrombus resolution from an earthworm extract using an immunoaffinity resin column prepared by binding an antibody against a protein having a thrombus ability of the earthworm to CNBr-activated Sepharose. 제1항에 있어서, 지렁이의 혈전분해능을 갖는 단백질에 대한 항체를 프로테인 A 세파로즈 칼럼을 이용하여정제됨을 특징으로 하는 방법.The method of claim 1, wherein the antibody against the protein having thrombolytic ability of the earthworm is purified using a Protein A Sepharose column. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930020673A 1993-10-06 1993-10-06 Purification process of proteins by using antibodies KR970002491B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930020673A KR970002491B1 (en) 1993-10-06 1993-10-06 Purification process of proteins by using antibodies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930020673A KR970002491B1 (en) 1993-10-06 1993-10-06 Purification process of proteins by using antibodies

Publications (2)

Publication Number Publication Date
KR950011465A true KR950011465A (en) 1995-05-15
KR970002491B1 KR970002491B1 (en) 1997-03-05

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ID=19365356

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019930020673A KR970002491B1 (en) 1993-10-06 1993-10-06 Purification process of proteins by using antibodies

Country Status (1)

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KR (1) KR970002491B1 (en)

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Publication number Publication date
KR970002491B1 (en) 1997-03-05

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