KR950008694A - Method for producing high purity isomaltooligosaccharide - Google Patents

Method for producing high purity isomaltooligosaccharide

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Publication number
KR950008694A
KR950008694A KR1019930017510A KR930017510A KR950008694A KR 950008694 A KR950008694 A KR 950008694A KR 1019930017510 A KR1019930017510 A KR 1019930017510A KR 930017510 A KR930017510 A KR 930017510A KR 950008694 A KR950008694 A KR 950008694A
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KR
South Korea
Prior art keywords
isomaltooligosaccharide
high purity
solution
sugar
producing high
Prior art date
Application number
KR1019930017510A
Other languages
Korean (ko)
Inventor
김정렬
육철
Original Assignee
안기영
두산종합식품 주식회사
성우경
두산기술연구조합
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 안기영, 두산종합식품 주식회사, 성우경, 두산기술연구조합 filed Critical 안기영
Priority to KR1019930017510A priority Critical patent/KR950008694A/en
Publication of KR950008694A publication Critical patent/KR950008694A/en

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Abstract

본 발명은 이소말토 올리고당의 제조방법에 관한 것으로, 좀 더 구체적으로는 맥아당액에 당전이 효소를 첨가하여 이소말토 올리고당액을 얻은 후, 상기 이소말토 올리고당액에 글루코아밀라제를 첨가한 다음, pH를 4.5∼6으로 조절하고 50∼70℃에서 10∼60 시간 반응시켜 직쇄올리고당의 함량을 감소시킨 다음, 이것을 크로마토그래피로 분리시켜 고순도로 이소말토 올리고당을 제조하는 방법에 관한 것이다.The present invention relates to a method for preparing isomaltooligosaccharide, and more specifically, to obtain a isomaltooligosaccharide solution by adding a sugar transfer enzyme to maltose solution, and then adding glucoamylase to the isomaltooligosaccharide solution and then adjusting the pH. The present invention relates to a method for producing isomaltooligosaccharide in high purity by controlling the content to 4.5 to 6 and reacting at 50 to 70 ° C for 10 to 60 hours to reduce the content of the linear oligosaccharide.

Description

고순도 이소말토 올리고당의 제조방법Method for producing high purity isomaltooligosaccharide

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (8)

맥아당액에 당전이 효소를 첨가하여 이소말토 올리고당액을 얻은 후, 상기 이소말토 올리고당액에 글루코아밀라제를 첨가한 다음, pH를 4.5∼6으로 조절하고 50-70℃에서 10∼60 시간 반응시켜 직쇄올리고당의 함량을 감소시킨 다음, 이것을 크로마토그래피로 분리시키는 것을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.After adding sugar transfer enzyme to maltose solution to obtain isomalto oligosaccharide solution, add glucoamylase to the isomalto oligosaccharide solution, adjust the pH to 4.5-6 and react at 10-60 hours at 50-70 ° C A method for producing high purity isomalto oligosaccharides, characterized in that the content of oligosaccharides is reduced and then separated by chromatography. 제1항에 있어서, 상기 맥아당액이 고형분내에 글루코스 중합도가 2이상인 당을 50% 이상 함유하는 당수용액으로써 고형분 농도 20% 이상으로 사용됨을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.The method for producing high purity isomaltooligosaccharide according to claim 1, wherein the maltose solution is a sugar aqueous solution containing 50% or more of sugar having a degree of glucose polymerization of 2 or more in solid content and used at a solid concentration of 20% or more. 제1항에 있어서, 상기 이소말토 올리고당액 고형분 100g에 대하여 글루코아밀라제 0.001∼0.5g을 첨가시키는 것을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.The method for producing a high purity isomaltooligosaccharide according to claim 1, wherein 0.001 to 0.5g of glucoamylase is added to 100g of the isomaltooligosaccharide solids. 제1항에 있어서, 상기 크로마토그래피의 매체로 강산성 양이온 교환수지를 사용함을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.The method of claim 1, wherein a strong acid cation exchange resin is used as a medium for the chromatography. 맥아당액에 당전이 효소와 글루코아밀라제를 동시에 첨가한 다음, pH를 4.5∼6으로 조절하고 50∼70℃에서 10∼60 시간 반응시켜 얻은 이소말토 올리고당액을 크로마토그래피로 분리시키는 것을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.High-purity characterized by separating the isomaltooligosaccharide solution obtained by adding a sugar transfer enzyme and glucoamylase simultaneously to the maltose solution, adjusting the pH to 4.5-6 and reacting at 50-70 ° C for 10-60 hours. Method for producing isomaltooligosaccharide. 제5항에 있어서, 상기 맥아당액이 고형분내에 글루코스 중합도가 2이상인 당을 50% 이상 함유하는 당수용액으로써, 고형분 농도 20% 이상으로 사용됨을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.The method for producing high purity isomaltooligosaccharide according to claim 5, wherein the maltose solution is a sugar aqueous solution containing 50% or more of sugar having a degree of glucose polymerization of 2 or more in solid content, and is used at a solid content concentration of 20% or more. 제5항에 있어서, 상기 맥아당액 고형분 100g에 대하여 당전이 효소 및 글루코아밀라제를 0.01∼0.5g 및 0.001~0.5g으로 각각 첨가시키는 것을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.The method for producing high purity isomaltooligosaccharide according to claim 5, wherein the sugar transfer enzyme and the glucoamylase are added in an amount of 0.01 to 0.5 g and 0.001 to 0.5 g, respectively, based on 100 g of the maltose liquid solid content. 제5항에 있어서, 상기 크로마토그래피의 매체로 강산성 양이온 교환수지를 사용함을 특징으로 하는 고순도 이소말토 올리고당의 제조방법.The method of claim 5, wherein a strong acid cation exchange resin is used as a medium for the chromatography. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930017510A 1993-09-02 1993-09-02 Method for producing high purity isomaltooligosaccharide KR950008694A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930017510A KR950008694A (en) 1993-09-02 1993-09-02 Method for producing high purity isomaltooligosaccharide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930017510A KR950008694A (en) 1993-09-02 1993-09-02 Method for producing high purity isomaltooligosaccharide

Publications (1)

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KR950008694A true KR950008694A (en) 1995-04-19

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KR1019930017510A KR950008694A (en) 1993-09-02 1993-09-02 Method for producing high purity isomaltooligosaccharide

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100387286B1 (en) * 2000-06-30 2003-06-12 주식회사 삼양제넥스 Method for production of isomaltooligosaccharides
KR100450619B1 (en) * 2002-04-22 2004-09-30 학교법인 인제학원 The screening method for the immunomodulating oligosaccharides extracted from natural material using programmed cell death
KR101228502B1 (en) * 2011-02-18 2013-01-31 대상 주식회사 Isomalto-oligosaccharide powder composition with low hygroscopicity and high flowability, and manufacturing method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100387286B1 (en) * 2000-06-30 2003-06-12 주식회사 삼양제넥스 Method for production of isomaltooligosaccharides
KR100450619B1 (en) * 2002-04-22 2004-09-30 학교법인 인제학원 The screening method for the immunomodulating oligosaccharides extracted from natural material using programmed cell death
KR101228502B1 (en) * 2011-02-18 2013-01-31 대상 주식회사 Isomalto-oligosaccharide powder composition with low hygroscopicity and high flowability, and manufacturing method thereof

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