KR950002767A - Low molecular weight-urogastron purification method - Google Patents
Low molecular weight-urogastron purification method Download PDFInfo
- Publication number
- KR950002767A KR950002767A KR1019930014841A KR930014841A KR950002767A KR 950002767 A KR950002767 A KR 950002767A KR 1019930014841 A KR1019930014841 A KR 1019930014841A KR 930014841 A KR930014841 A KR 930014841A KR 950002767 A KR950002767 A KR 950002767A
- Authority
- KR
- South Korea
- Prior art keywords
- molecular weight
- urogastron
- column chromatography
- egf
- low molecular
- Prior art date
Links
- 238000000746 purification Methods 0.000 title claims abstract description 5
- 238000000034 method Methods 0.000 title claims abstract 12
- 238000004440 column chromatography Methods 0.000 claims abstract 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract 4
- 238000000108 ultra-filtration Methods 0.000 claims abstract 4
- 238000005342 ion exchange Methods 0.000 claims abstract 3
- 238000001556 precipitation Methods 0.000 claims abstract 3
- 239000003463 adsorbent Substances 0.000 claims abstract 2
- 230000008021 deposition Effects 0.000 claims abstract 2
- 238000000605 extraction Methods 0.000 claims abstract 2
- 238000001179 sorption measurement Methods 0.000 claims abstract 2
- 210000002700 urine Anatomy 0.000 claims abstract 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 2
- 239000011780 sodium chloride Substances 0.000 claims 2
- 239000004471 Glycine Substances 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000000706 filtrate Substances 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
본 발명은 사람이나 동물의 뇨를 흡착제를 사용한 흡착, 에탄올-분별침전, 다단계 이온교환 컬럼크로마토그래피 및 항-EGF 컬럼크로마토그래피를 순차적으로 행함으로써 저분자량의 우로가스트론을 정제하는 방법에 있어서, 에탄올-분별침전후, 상대적으로 간단한 공정인 추출 및 한외여과단계, 일단계의 컬럼크로마토그래피, 아세톤 침적, 항-EGF컬럼크로마토그래피를 거침으로써 컬럼크로마토그래피 공정을 최소화하여 저분자량-우로가스트론을 고수율로 간편하게 정제하는 것을 특징으로 하는 분자량 6,000의 저분자량-우로가스트론 정제방법에 관한 것이다.The present invention provides a method for purifying low molecular weight urogastron by sequentially performing human or animal urine adsorption using an adsorbent, ethanol-fractionation precipitation, multistage ion exchange column chromatography and anti-EGF column chromatography. After ethanol-separation precipitation, low molecular weight-urogastron is minimized by minimizing column chromatography process through relatively simple process of extraction and ultrafiltration, one step column chromatography, acetone deposition, and anti-EGF column chromatography. The present invention relates to a low molecular weight-urogastron purification method having a molecular weight of 6,000, which is characterized by simple purification in high yield.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 표준 시료(Standard EGF)로 부터 구한 검량선을 나타낸 도면이고, 제2도는 정제단계로부터 얻어진 분획의 전기영동 사진을 나타낸 도면이다.FIG. 1 is a diagram showing a calibration curve obtained from a standard sample (Standard EGF), and FIG. 2 is a diagram showing electrophoresis images of fractions obtained from a purification step.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930014841A KR0163209B1 (en) | 1993-07-31 | 1993-07-31 | Process for purification of high molecular weight-urogastron |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930014841A KR0163209B1 (en) | 1993-07-31 | 1993-07-31 | Process for purification of high molecular weight-urogastron |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR950002767A true KR950002767A (en) | 1995-02-16 |
| KR0163209B1 KR0163209B1 (en) | 1998-11-16 |
Family
ID=19360558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019930014841A KR0163209B1 (en) | 1993-07-31 | 1993-07-31 | Process for purification of high molecular weight-urogastron |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR0163209B1 (en) |
-
1993
- 1993-07-31 KR KR1019930014841A patent/KR0163209B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR0163209B1 (en) | 1998-11-16 |
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| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant | ||
| FPAY | Annual fee payment |
Payment date: 20030204 Year of fee payment: 6 |
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| LAPS | Lapse due to unpaid annual fee |