KR950000732A - Method for preparing chitin and chitosan for biological clinical medicine by low temperature treatment - Google Patents

Method for preparing chitin and chitosan for biological clinical medicine by low temperature treatment Download PDF

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KR950000732A
KR950000732A KR1019930011599A KR930011599A KR950000732A KR 950000732 A KR950000732 A KR 950000732A KR 1019930011599 A KR1019930011599 A KR 1019930011599A KR 930011599 A KR930011599 A KR 930011599A KR 950000732 A KR950000732 A KR 950000732A
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South Korea
Prior art keywords
treatment
aqueous solution
hydrochloric acid
chitin
drying
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KR1019930011599A
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Korean (ko)
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KR970009897B1 (en
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전동원
김신희
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전동원
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
    • C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
    • C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Sustainable Development (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

본 발명은 게 갑각으로부터 고순도 및 고백색도로 키틴을 제조할 수 있는 방법을 제공하며, 또한 이로부터 고탈아세틸화도 및 고분자량의 키토산을 제조할 수 있는 방법을 제공한다. 구체적으로, 본 발명의 키틴 제조방법은 게 갑각을 건조하고 분쇄시켜 -40 내지 -10℃에서 3 내지 20시간 동안 1.5 내지 7N 염산 수용액으로 처리한 후 수세, 여과, 용매처리 및 건조하고, 이어서 NaOH 수용액으로 처리한 후, 수세, 여과, 및 건조하는 단계들을 포함한다.The present invention provides a method for preparing chitin with high purity and high whiteness from crab shell, and also provides a method for producing chitosan having high deacetylation degree and high molecular weight therefrom. Specifically, the method for preparing chitin of the present invention is dried and ground crab shell, treated with 1.5 to 7N aqueous hydrochloric acid solution at -40 to -10 ℃ for 3 to 20 hours, followed by washing with water, filtration, solvent treatment and drying, followed by NaOH Treatment with an aqueous solution, followed by washing with water, filtration, and drying.

Description

저온처리에 의한 생체 임상의학용 키틴 및 키토산의 제조방법Method for preparing chitin and chitosan for biological clinical medicine by low temperature treatment

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (8)

(1) 게 갑각을 건조시켜 보관하는 단계, (2) 건조된 게 갑각을 분쇄시키는 단계, (3) 분쇄된 게 갑각을 1.5 내지 7N의 염산 수용액으로 -40 내지 -10℃의 온도에서 3 내지 20시간 동안 처리하는 단계, (4)염산 처리된 게 갑각을 수세, 여과, 용매처리 및 건조하여 조 키틴을 수득하는 단계, (5) 건조된 조 키틴을 NaOH 수용액에 침지시켜 가열처리하는 단계 및 (6) NaOH 처리된 키틴을 수세, 여과 및 건조시키는 단계를 포함하는, 게 갑각으로부터 키틴을 제조하는 방법.(1) drying and storing the crab shell, (2) crushing the dried crab shell, and (3) grinding the crab shell with the aqueous solution of hydrochloric acid of 1.5 to 7N at a temperature of -40 to -10 ° C. 20 hours of treatment, (4) washing the hydrochloric acid-treated crab shell with water, filtration, solvent treatment, and drying to obtain crude chitin, (5) heat treatment by dipping the dried crude chitin in aqueous NaOH solution, and (6) A method of making chitin from crab crust, which comprises washing, filtering and drying NaOH treated chitin. 제 1 항에 있어서, 상기 염산 처리 단계에서 처리 온도가 -35 내지 -10℃인 방법.The process of claim 1 wherein the treatment temperature in the hydrochloric acid treatment step is -35 to -10 ° C. 제 1 항에 있어서, 상기 염산처리 단계에 사용하기 위한 염산 수용액을, 농염산, 탈이온수 및 유기 용매를 혼합함으로써 제조하는 방법.The method according to claim 1, wherein an aqueous hydrochloric acid solution for use in the hydrochloric acid treatment step is prepared by mixing concentrated hydrochloric acid, deionized water and an organic solvent. 제 3 항에 있어서, 상기 유기 용매가 에탄올, 메탄올, 부탄올, 이소프로필알콜, 아세톤 또는 메틸에틸케톤인 방법.4. The process according to claim 3, wherein the organic solvent is ethanol, methanol, butanol, isopropyl alcohol, acetone or methyl ethyl ketone. 제 1 항의 방법에 의해 수득된 키틴으로부터 탈아세탈화법에 의해 키토산을 제조하는 방법.A method for producing chitosan by deacetalization from chitin obtained by the method of claim 1. 제 5 항에 있어서, 상기 탈아세틸화법이 NaOH 수용액으로 80 내지 100℃의 온도에서 2 내지 12시간 동안 가열 처리한 다음, 수세 및 여과하는 단계, 수득된 조 키토산을 탈 이온수에 첨지시킨 후 여과하여 유기 용매의 수용액에 침지시킨 다음 여과 및 건조하는 단계 및 상기 NaOH 수용액 처리 및 유기 용매처리 공정을 반복하는 단계를 포함하는 방법.The method of claim 5, wherein the deacetylation method is heat-treated with NaOH aqueous solution at a temperature of 80 to 100 ℃ for 2 to 12 hours, followed by washing with water and filtration, the crude chitosan obtained is impregnated in deionized water and then filtered Immersing in an aqueous solution of an organic solvent, followed by filtration and drying, and repeating the NaOH aqueous solution treatment and the organic solvent treatment process. 제 6 항에 있어서, 상기 유기 용매의 수용액의 아세톤, 에탄올, 메탄올, 이소프로판올, 디옥산, 테트라히드로푸란 또는 메틸에틸케톤의 5 내지 30중량% 수용액인 방법.The method according to claim 6, which is a 5 to 30% by weight aqueous solution of acetone, ethanol, methanol, isopropanol, dioxane, tetrahydrofuran or methylethylketone in the aqueous solution of the organic solvent. 제 6 항에 있어서, 상기 NaOH 수용액 처리를 불활성 대기하에 수행하는 방법.7. The method of claim 6, wherein said NaOH aqueous solution treatment is performed under an inert atmosphere. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930011599A 1993-06-24 1993-06-24 Preparation process of medical chitin and chitosan using low temperature treatment KR970009897B1 (en)

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KR1019930011599A KR970009897B1 (en) 1993-06-24 1993-06-24 Preparation process of medical chitin and chitosan using low temperature treatment

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KR950000732A true KR950000732A (en) 1995-01-03
KR970009897B1 KR970009897B1 (en) 1997-06-19

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6740402B2 (en) 2000-07-14 2004-05-25 Teijin Limited Polyester fiber

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6740402B2 (en) 2000-07-14 2004-05-25 Teijin Limited Polyester fiber

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