KR940014780A - Method for preparing an active form of growth hormone from inclusion bodies of growth hormone - Google Patents

Method for preparing an active form of growth hormone from inclusion bodies of growth hormone Download PDF

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Publication number
KR940014780A
KR940014780A KR1019920025905A KR920025905A KR940014780A KR 940014780 A KR940014780 A KR 940014780A KR 1019920025905 A KR1019920025905 A KR 1019920025905A KR 920025905 A KR920025905 A KR 920025905A KR 940014780 A KR940014780 A KR 940014780A
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KR
South Korea
Prior art keywords
growth hormone
active form
preparing
reoxidation
inclusion bodies
Prior art date
Application number
KR1019920025905A
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Korean (ko)
Inventor
제훈성
이홍균
임관열
Original Assignee
최근선
주식회사 럭키
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 최근선, 주식회사 럭키 filed Critical 최근선
Priority to KR1019920025905A priority Critical patent/KR940014780A/en
Publication of KR940014780A publication Critical patent/KR940014780A/en

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Abstract

본 발명은 유전공학적인 기술에 의해 DNA 재조합 되어진 숙주세포에서 발현된 성장호르몬의 봉입체로부터 활성형태의 성장 호르몬을 제조함에 있어서, 성장호르몬의 봉입체를 강염기성 pH에서 용해시킨 후 용소를 첨가한 상태에서 재중첩 및 재산화시키는 단계를 포함하는 것을 특징으로 하는 활성형태의 성장호르몬을 제조하는 방법을 제공한다.The present invention, in the production of an active form of growth hormone from the growth hormone inclusion body expressed in the DNA cell recombinant DNA by genetic engineering techniques, in the state in which the growth hormone was dissolved at a strong base pH, and then dissolved It provides a method for producing an active form of growth hormone, characterized in that it comprises the step of re-overlap and reoxidation.

Description

성장호르몬의 봉입체로부터 활성형태의 성장호르몬을 제조하는 방법.A method for producing an active form of growth hormone from inclusion bodies of growth hormone.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따라 재중첩 및 재산화 한 후의 HPLC 크로마토 그램이다.1 is an HPLC chromatogram after re-overlap and reoxidation according to the present invention.

Claims (5)

재조합 숙주세포에서 발현된 성장호르몬의 봉입체로부터 활성형태의 성장호르몬을 제조함에 있어서, 성장 호르몬의 봉입체를 강염기성 pH에서 용해시킨 후 요소를 첨가한 상태에서 재중첩 및 재산화시키는 단계를 포함하는 것을 특징으로 하는 활성형태의 성장호르몬을 제조하는 방법.In preparing an active form of growth hormone from an inclusion body of growth hormone expressed in a recombinant host cell, dissolving the growth hormone inclusion at a strong basic pH, and then re-overlapping and reoxidation with urea added. Method for producing an active form of growth hormone. 제1항에 있어서, 상기 강염기성 pH는 12 내지 13인 방법.The method of claim 1, wherein the strongly basic pH is 12 to 13. 제1항에 있어서, 재중첩 및 재산화시키는 단계에서 상기 첨가되는 요소농도는 1M 내지 3M인 방법.The method of claim 1 wherein the added urea concentration in the re-overlap and reoxidation step is 1M to 3M. 제1항에 있어서, 재중첩 및 재산화시의 온도는 0℃ 내지 15℃인 방법.The method of claim 1 wherein the temperature at re-overlap and reoxidation is from 0 ° C. to 15 ° C. 제1항에 있어서, 상기 성장호르몬은 소 또는 돼지의 성장호르몬인 방법.The method of claim 1, wherein the growth hormone is a growth hormone of cattle or pigs. ※참고사항:최초출원 내용에 의하여 공개하는 것임.※ Note: This is to be disclosed based on the first application.
KR1019920025905A 1992-12-28 1992-12-28 Method for preparing an active form of growth hormone from inclusion bodies of growth hormone KR940014780A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920025905A KR940014780A (en) 1992-12-28 1992-12-28 Method for preparing an active form of growth hormone from inclusion bodies of growth hormone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920025905A KR940014780A (en) 1992-12-28 1992-12-28 Method for preparing an active form of growth hormone from inclusion bodies of growth hormone

Publications (1)

Publication Number Publication Date
KR940014780A true KR940014780A (en) 1994-07-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019920025905A KR940014780A (en) 1992-12-28 1992-12-28 Method for preparing an active form of growth hormone from inclusion bodies of growth hormone

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KR (1) KR940014780A (en)

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