KR940014780A - Method for preparing an active form of growth hormone from inclusion bodies of growth hormone - Google Patents
Method for preparing an active form of growth hormone from inclusion bodies of growth hormone Download PDFInfo
- Publication number
- KR940014780A KR940014780A KR1019920025905A KR920025905A KR940014780A KR 940014780 A KR940014780 A KR 940014780A KR 1019920025905 A KR1019920025905 A KR 1019920025905A KR 920025905 A KR920025905 A KR 920025905A KR 940014780 A KR940014780 A KR 940014780A
- Authority
- KR
- South Korea
- Prior art keywords
- growth hormone
- active form
- preparing
- reoxidation
- inclusion bodies
- Prior art date
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Abstract
본 발명은 유전공학적인 기술에 의해 DNA 재조합 되어진 숙주세포에서 발현된 성장호르몬의 봉입체로부터 활성형태의 성장 호르몬을 제조함에 있어서, 성장호르몬의 봉입체를 강염기성 pH에서 용해시킨 후 용소를 첨가한 상태에서 재중첩 및 재산화시키는 단계를 포함하는 것을 특징으로 하는 활성형태의 성장호르몬을 제조하는 방법을 제공한다.The present invention, in the production of an active form of growth hormone from the growth hormone inclusion body expressed in the DNA cell recombinant DNA by genetic engineering techniques, in the state in which the growth hormone was dissolved at a strong base pH, and then dissolved It provides a method for producing an active form of growth hormone, characterized in that it comprises the step of re-overlap and reoxidation.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 본 발명에 따라 재중첩 및 재산화 한 후의 HPLC 크로마토 그램이다.1 is an HPLC chromatogram after re-overlap and reoxidation according to the present invention.
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019920025905A KR940014780A (en) | 1992-12-28 | 1992-12-28 | Method for preparing an active form of growth hormone from inclusion bodies of growth hormone |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019920025905A KR940014780A (en) | 1992-12-28 | 1992-12-28 | Method for preparing an active form of growth hormone from inclusion bodies of growth hormone |
Publications (1)
Publication Number | Publication Date |
---|---|
KR940014780A true KR940014780A (en) | 1994-07-19 |
Family
ID=67215127
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920025905A KR940014780A (en) | 1992-12-28 | 1992-12-28 | Method for preparing an active form of growth hormone from inclusion bodies of growth hormone |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR940014780A (en) |
-
1992
- 1992-12-28 KR KR1019920025905A patent/KR940014780A/en not_active Application Discontinuation
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A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E601 | Decision to refuse application |