KR940011627A - Pleuurotus Austratus producing Ascorbic Acid - Google Patents

Pleuurotus Austratus producing Ascorbic Acid Download PDF

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KR940011627A
KR940011627A KR1019920020727A KR920020727A KR940011627A KR 940011627 A KR940011627 A KR 940011627A KR 1019920020727 A KR1019920020727 A KR 1019920020727A KR 920020727 A KR920020727 A KR 920020727A KR 940011627 A KR940011627 A KR 940011627A
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South Korea
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austratus
acid
ascorbic acid
pleurotus
pleuurotus
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KR1019920020727A
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Korean (ko)
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강사욱
권재열
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하영칠
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Priority to KR1019920020727A priority Critical patent/KR940011627A/en
Publication of KR940011627A publication Critical patent/KR940011627A/en

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Abstract

플루우로투스 오스트레아투스 2m1은 L-아스콜빈산을 생산하는 신규한 미생물로서, L-글로노-γ-락톤을 기질로하여 L-아스콜빈산을 생산할수 있으며, 산소 공급하에서 L-아스콜빈산을 많이 생성한다. 또한 이 미생물을 L-아스콜빈산의 전구체를 포함하는 완충용액에 투입하여 L-아스콜빈산을 생합성할 수 있다. 이와같은 생합성 방법은 종래의 L-아스콜빈산의 화학합성 및 생합성에 비하여 공정이 간단하고 경제적이다.Fluorotus Austratus 2m1 is a novel microorganism that produces L-ascovinic acid, which can produce L-ascovinic acid based on L-glono-γ-lactone as a substrate, and L-ascolvin under oxygen supply. Produces a lot of acid. In addition, the microorganisms may be added to a buffer containing a precursor of L-ascorbic acid to biosynthesize L-ascorbic acid. This biosynthesis method is simpler and more economical than the conventional chemical synthesis and biosynthesis of L-ascorbic acid.

Description

아스콜빈산을 생산하는 플레우로투스 오스트레아투스Pleuurotus Austratus producing Ascorbic Acid

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 효모등의 아스콜빈산 생합성 경로를 나타내는 설명도이고, 제2도는 박테리아의 아스콜빈산 생합성 경로를 나타내는 설명도이고, 제3도는 본 발명의 미생물을 최소배지+L-글로노-γ-락톤연산 완충용액의 배지에 공기를 주입하면서 배양한 배양액에 아스콜빈산 옥시다제를 처리했을 때의 흡광도의 변화를 나타낸 그래프이다.1 is an explanatory diagram showing the ascorbic acid biosynthetic pathways such as yeast, FIG. 2 is an explanatory diagram showing the ascorbic acid biosynthetic pathway of bacteria, and FIG. 3 is a minimal medium + L-glono-γ of the microorganism of the present invention It is a graph showing the change in absorbance when treated with ascorbic acid oxidase in culture cultured while injecting air into the lactone acetate buffer medium.

Claims (11)

KCTC 기탁번호 제KCTC0050BP호의 플레우로투스 오스트레아투스.Pleuurotus Austratus in KCTC Accession No. KCTC0050BP. L-아스콜빈산을 생산하는 플레우로투스 오스트레아투스.Pleuurotus austratus producing L-ascorbic acid. 제2항에 있어서, 상기한 플레우로투스 오스트레아투스는 L-글로노-γ-락톤을 L-아스콜빈산을 전환시키는 것을 특징으로 하는 플레이오로투스 오스트레아투스.3. Pluorotus austratus according to claim 2, characterized in that the pleurotus austratus converts L-glono- [gamma] -lactone to L-ascolic acid. 플레우로투스 오스트레아투스를 배양하여 L-아스콜빈산을 생산하는 방법.A method of culturing Pleurotus austratus to produce L-ascolic acid. 제4항에 있어서, 상기한 플레우로투스 오스트레아투스를 L-글로노-γ-락톤을 포함하는 배지에서 배양하는 것을 특징으로 하는 L-아스콜빈산을 생산하는 방법.The method of claim 4, wherein the Pleurotus austratus is cultured in a medium containing L-glono-γ-lactone. 제4항에 있어서, 상기한 플레우로투스 오스트레아투스를 공기를 주입하면서 배양하는 것을 특징으로 하는 L-아스콜빈산을 생산하는 방법.The method of claim 4, wherein the pleurotus austratus is cultured while injecting air. L-아스콜빈산을 생산하는 플레이오로투스 오스트레아투스를 L-아스콜빈산 전구체를 포함하는 완충용액에 투입하여 L-아스콜빈산을 생산하는 방법.A method for producing L-ascovinic acid by injecting P. austratusus that produces L-ascolic acid into a buffer containing an L-ascoric acid precursor. 제7항에 있어서, 상기한 완충용액은 구연산 완충용액인 것을 특징으로 하는 L-아스콜빈산을 생산하는 방법.The method of claim 7, wherein the buffer solution is citric acid buffer solution. 제7항에 있어서, 상기한 L-아스콜빈산의 전구체는 L-글로노-γ-락톤인 것을 특징으로 하는 L-아스콜빈산을 생산하는 방법.8. The method of claim 7, wherein the precursor of L-ascorbic acid is L-glono-γ-lactone. 제7항에 있어서, 상기한 플레우로투스 오스트레아투스는 최소 배지에서 배양한 것임을 특징으로 하는 L-아스콜빈산을 생산하는 방법.The method of claim 7, wherein the Pleurotus austratus is cultured in a minimal medium. 제7항에 있어서, 상기한 완충용액은 pH6∼8인 것을 특징으로 하는 L-아스콜빈산을 생산하는 방법.The method of claim 7, wherein the buffer solution has a pH of 6 to 8. 8. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920020727A 1992-11-05 1992-11-05 Pleuurotus Austratus producing Ascorbic Acid KR940011627A (en)

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