KR940003567A - Method for preparing intravenous gamma globulin with immunoglobulins A and M removed - Google Patents

Method for preparing intravenous gamma globulin with immunoglobulins A and M removed Download PDF

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Publication number
KR940003567A
KR940003567A KR1019920014494A KR920014494A KR940003567A KR 940003567 A KR940003567 A KR 940003567A KR 1019920014494 A KR1019920014494 A KR 1019920014494A KR 920014494 A KR920014494 A KR 920014494A KR 940003567 A KR940003567 A KR 940003567A
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KR
South Korea
Prior art keywords
polyethylene glycol
sodium chloride
immunoglobulins
buffer solution
gamma globulin
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Application number
KR1019920014494A
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Korean (ko)
Inventor
이명규
김수연
김길룡
변광호
함경수
Original Assignee
서정옥
한국과학기술연구원
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Application filed by 서정옥, 한국과학기술연구원 filed Critical 서정옥
Priority to KR1019920014494A priority Critical patent/KR940003567A/en
Publication of KR940003567A publication Critical patent/KR940003567A/en

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Abstract

본 발명은 콘 분획 II 또는 II+III페이스트를 풀리에틸렌글리콜-염화나트륨-초산염 완충응액에 현탁한 후 용해되지 않는 응집체를 제거하고, DEAE-이온 교환 수지 크로마토그래피하는 단계를 포함하는 면역글로불린 A 및 M이 제거된 정맥주사용 강마글로불린제의 제조방법을 제공하는 것이다.The present invention comprises immunoglobulin A and M comprising suspending the cone fraction II or II + III paste in pulleyethylene glycol-sodium chloride-acetate buffer, removing the insoluble aggregates, and chromatography with DEAE-ion exchange resin. It is to provide a method for producing the removed intravenous gangmaglobulin agent.

Description

면역글로불린 A 및 7이 제거된 정맥주사용 감마글로불린제의 제조방법Method for preparing intravenous gamma globulin with immunoglobulins A and 7 removed

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1(가)도는 본 발명에 따라 폴리에틸렌글리콜 처리후 DE.7E-이온교환 크로마토그래피전에 얻어진 시료를 겔 여과 크로마토그래피한 겪과이고, 제1 (나)도는 본 발명에 따라 폴리에틸렌 글리콜 처리 및 DEAE-이온교환 크로마토그래피후에 얻어진 시료를 겔 여과 크로마토그래피한 결과이며,Figure 1 (a) shows the result of gel filtration chromatography of the sample obtained before the DE.7E-ion exchange chromatography after polyethylene glycol treatment according to the present invention, and Figure 1 (b) shows the polyethylene glycol treatment and DEAE according to the present invention. It is the result of the gel filtration chromatography of the sample obtained after ion exchange chromatography,

제2도는 제1(나)도에서의 7s분획을 SDS-변성 전기영동한 결과이다.Figure 2 is the result of SDS-modified electrophoresis of the 7s fraction in Figure 1 (b).

Claims (5)

큰 분획 II 또는 II+III페이스트를 폴리에틸렌글리콜-염화 나트륨-초산염 완충응액에 현탁한후 용해되지 않는 응집테를 제거하는 단계 및 DEAE-이온 교환 수지 크로마토그래피하는 단계를 포함하는, 면역글로불린 A및 M이 제거된 정맥주사용 감마글로불린제의 제조방법.Immunoglobulins A and M, comprising suspending a large fraction II or II + III paste in polyethylene glycol-sodium chloride-acetate buffer solution followed by removal of insoluble aggregate and DEAE-ion exchange resin chromatography A method for producing the removed intravenous gamma globulin agent. 제1항에 있어서, 상기 폴리에틸렌글리콜-염화나트륨-초산염 완충용액은 폴리 에틸렌글리콜의 농도가 4내지 6%이고 pH가 4.6 내지 5.4인 방법.The method according to claim 1, wherein the polyethylene glycol-sodium chloride-acetate buffer solution has a concentration of polyethylene glycol of 4 to 6% and a pH of 4.6 to 5.4. 제1항에 있어서, 상기 BEAE-이온 교환 크로마토그래피는 인산나트륨의 농도가 0.015 내지 0.025M이고 pH가 4.6 내지 5.4인 인산나트륨 완충용액을 사용하는 방법.The method of claim 1, wherein the BEAE-ion exchange chromatography uses sodium phosphate buffer solution having a sodium phosphate concentration of 0.015 to 0.025M and a pH of 4.6 to 5.4. 제1항에 있어서, 상기 폴리에틸렌글리콜은 분자량이 408? 내지 8000인 것을 사용하는 방법.The method of claim 1, wherein the polyethylene glycol has a molecular weight of 408? To 8000. 제1항에 있어서, 상기 크로마토그래피 단계후 단백질 용액의 PH를 7.0 내지 7.5로 조절한후 염화나트륨 및 폴리에틸렌글리콜을 첨가하고 원심분리하여 힐전물을 얻은 단계를 추가로 포함하는 방법.The method of claim 1, further comprising adjusting the pH of the protein solution after the chromatography step to 7.0 to 7.5, and then adding sodium chloride and polyethylene glycol and centrifuging to obtain a heel preparation. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920014494A 1992-08-12 1992-08-12 Method for preparing intravenous gamma globulin with immunoglobulins A and M removed KR940003567A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920014494A KR940003567A (en) 1992-08-12 1992-08-12 Method for preparing intravenous gamma globulin with immunoglobulins A and M removed

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920014494A KR940003567A (en) 1992-08-12 1992-08-12 Method for preparing intravenous gamma globulin with immunoglobulins A and M removed

Publications (1)

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KR940003567A true KR940003567A (en) 1994-03-12

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