KR940002610A - Anti-nuclear antibody diagnostic slide and its manufacturing method - Google Patents

Anti-nuclear antibody diagnostic slide and its manufacturing method Download PDF

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Publication number
KR940002610A
KR940002610A KR1019920012928A KR920012928A KR940002610A KR 940002610 A KR940002610 A KR 940002610A KR 1019920012928 A KR1019920012928 A KR 1019920012928A KR 920012928 A KR920012928 A KR 920012928A KR 940002610 A KR940002610 A KR 940002610A
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KR
South Korea
Prior art keywords
slide
nuclear antibody
antibody diagnostic
cell line
manufacturing
Prior art date
Application number
KR1019920012928A
Other languages
Korean (ko)
Other versions
KR950011547B1 (en
Inventor
김신규
Original Assignee
김신규
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 김신규 filed Critical 김신규
Priority to KR1019920012928A priority Critical patent/KR950011547B1/en
Priority to ES93916262T priority patent/ES2156127T3/en
Priority to PCT/KR1993/000061 priority patent/WO1994002594A1/en
Priority to AU45871/93A priority patent/AU4587193A/en
Priority to JP50436094A priority patent/JP3721581B2/en
Priority to DE69330160T priority patent/DE69330160T2/en
Priority to EP93916262A priority patent/EP0604635B1/en
Priority to US08/196,220 priority patent/US5583053A/en
Publication of KR940002610A publication Critical patent/KR940002610A/en
Application granted granted Critical
Publication of KR950011547B1 publication Critical patent/KR950011547B1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Abstract

본 발명은 정상인의 골수에서 유래된 IT-1 세포주(KFCC-10772)를 사용하여, 혈청내 항핵항체를 확인함으로써 전신성 류마티스등의 자가면역 질환을 진단하는 슬라이드에 관한 것으로 부착력이 뛰어나 진단용 IF 슬라이드 불량률이 낮아졌고, 접종량과 사용배지의 양도 절감되며 간단히 고정할 수 있다.The present invention relates to a slide for diagnosing autoimmune diseases such as systemic rheumatoid by identifying antinuclear antibodies in serum using IT-1 cell line (KFCC-10772) derived from bone marrow of a normal person. This lowers the amount of inoculation and medium used and can be fixed simply.

Description

항핵항체 진단용 슬라이드 및 그의 제작방법Anti-nuclear antibody diagnostic slide and its manufacturing method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

부착성의, 정상인의 골수로부터 유래된 신규의 항핵항체진단용 세포주를 IF슬라이드 구멍에 고정시켜서 이루어진 항핵항체 진단용 슬라이드.An anti-nuclear antibody diagnostic slide formed by fixing a novel anti-nuclear antibody diagnostic cell line derived from an adherent bone marrow to an IF slide hole. 제1항에 있어서, 세포주는 단핵세포(monocyte/macrophage) IT-1 (KFCC-10772)임을 특징으로 하는 항핵항체 진단용 슬라이드.The anti-nuclear antibody diagnostic slide according to claim 1, wherein the cell line is monocyte / macrophage IT-1 (KFCC-10772). 정상인의 골수로부터 유래된 신규의 부착성 항핵항체 진단용 세포주 IF-1 (KFCC-10772)를 슬라이드 구멍에 접종하여 24시간 배양한 후 완충액으로 세척한 뒤 고정시킴을 특징으로 하는 항핵항체 진단용 슬라이드.A novel anti-nuclear antibody diagnostic cell line IF-1 (KFCC-10772) derived from normal bone marrow inoculated into a slide hole, incubated for 24 hours, and then washed with buffer and fixed for anti-nuclear antibody diagnostic slide. 제3항에 있어서, 구멍당 104cell/㎖을 30마이크로리터로 접종함을 특징으로 하는 항핵항체 진단용 슬라이드의 제작 방법.4. The method of claim 3, wherein 10 4 cells / ml per hole are inoculated at 30 microliters. 제3항에 있어서, 메탄올로 세포를 고정함을 특징으로 하는 항핵항체 진단용 슬라이드의 제작 방법.The method for producing a slide for diagnosing antinuclear antibody according to claim 3, wherein the cell is fixed with methanol. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920012928A 1992-07-21 1992-07-21 Slide and its manufacturing method on anti-nuclear antibody diagnosis KR950011547B1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
KR1019920012928A KR950011547B1 (en) 1992-07-21 1992-07-21 Slide and its manufacturing method on anti-nuclear antibody diagnosis
ES93916262T ES2156127T3 (en) 1992-07-21 1993-07-21 MACROFAGOS CELL LINE FOR AN ANTI-BODY ANTIBODY TEST; A METHOD TO DETECT SUCH ANTIBODIES USING THE MACROPHAGES CELL LINE AND A PREPARATION FOR MICROSCOPE.
PCT/KR1993/000061 WO1994002594A1 (en) 1992-07-21 1993-07-21 Macrophage cell line for an antinuclear antibody test; a method for detecting the said antibody by employing the macrophage cell line and a slide preparation
AU45871/93A AU4587193A (en) 1992-07-21 1993-07-21 Macrophage cell line for an antinuclear antibody test; a method for detecting the said antibody by employing the macrophage cell line and a slide preparation
JP50436094A JP3721581B2 (en) 1992-07-21 1993-07-21 Macrophage cell line for antinuclear antibody test, antibody detection method using macrophage cell line and slide preparation
DE69330160T DE69330160T2 (en) 1992-07-21 1993-07-21 MACROPHAGE CELL LINE FOR ANTINUCLEAR ANTIBODY TEST; A METHOD FOR DETECTING THE SAME ANTIBODY BY USING THIS CELL LINE AND MANUFACTURING SLATS
EP93916262A EP0604635B1 (en) 1992-07-21 1993-07-21 Macrophage cell line for an antinuclear antibody test; a method for detecting the said antibody by employing the macrophage cell line and a slide preparation
US08/196,220 US5583053A (en) 1992-07-21 1993-07-21 Macrophage cell line for use in the detection of antinuclear antibodies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920012928A KR950011547B1 (en) 1992-07-21 1992-07-21 Slide and its manufacturing method on anti-nuclear antibody diagnosis

Publications (2)

Publication Number Publication Date
KR940002610A true KR940002610A (en) 1994-02-17
KR950011547B1 KR950011547B1 (en) 1995-10-06

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KR1019920012928A KR950011547B1 (en) 1992-07-21 1992-07-21 Slide and its manufacturing method on anti-nuclear antibody diagnosis

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100444366B1 (en) * 2001-07-27 2004-08-16 가부시키가이샤 히타치세이사쿠쇼 Image display device and color temperature correction method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100444366B1 (en) * 2001-07-27 2004-08-16 가부시키가이샤 히타치세이사쿠쇼 Image display device and color temperature correction method

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KR950011547B1 (en) 1995-10-06

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