KR940002531B1 - Process for making raw of soysauce by extruder - Google Patents

Process for making raw of soysauce by extruder Download PDF

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KR940002531B1
KR940002531B1 KR1019910025892A KR910025892A KR940002531B1 KR 940002531 B1 KR940002531 B1 KR 940002531B1 KR 1019910025892 A KR1019910025892 A KR 1019910025892A KR 910025892 A KR910025892 A KR 910025892A KR 940002531 B1 KR940002531 B1 KR 940002531B1
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soy sauce
extruder
extruded
soysauce
yeast extract
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KR1019910025892A
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KR930011884A (en
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정헌웅
오경근
김재철
박지용
임재각
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제일제당 주식회사
김정순
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/50Soya sauce

Abstract

A raw material for soysauce is prepd. by (a) mixing defatted soybean with 0.05-1 % yeast extract or 0.05-1.5 % hydrolysed vegetable protein (HVP) and (b) extruding the mixt. at 110-190 deg.C of barrel temp., 10-50 % of initial moisture content and 250-400 rpm velosity of screw to obtain the final product. The obtd. raw material for soysauce is useful for the improvement of efficiency of soysauce fermentation.

Description

익스트루더(extruder)를 이용한 양조 간장용 원료의 제조방법Manufacturing method of raw material for brewing soy sauce using extruder

본 발명은 익스트루더(extruder)를 이용, 고온 압출처리시에 단백 분해물을 첨가함으로써 탈지대두의 양조간장 제조 적성을 향상시킨 간장용 원료의 제조방법에 관한 것이다.The present invention relates to a method for producing soy sauce raw materials which improves the brewing soy sauce manufacturing aptitude of skim soybean by adding a protein decomposition product at the time of high temperature extrusion using an extruder.

양조간장은 장시간 발효, 숙성시키므로 시설규모가 크고 넓은 장소가 필요하게 되어 제조경비가 많이 들어가고 원료 이용률이 낮다. 이때의 원료 이용율은 70% 정도로 기온 및 환경조건에 따라 차이가 생길 수 있다.The brewed soy sauce is fermented and matured for a long time, so the facility is large and a large place is required, so the manufacturing cost is high and the utilization rate of raw materials is low. At this time, the utilization rate of raw materials may be about 70%, depending on the temperature and environmental conditions.

양조간장의 맛은 발효, 숙성 과정에서 생성되는데 전분질은 국균이 분비하는 효소작용에 의해 당분으로 분해되며, 단백질은 효소작용에 의해 펩타이드 및 아미노산으로 분해된다. 동시에 장류에 관계하는 효모에 의해 서서히 발효 작용을 일으키면서 소량의 알콜과 탄산가스를 발생하게 되며, 맛에 관여하는 유기산류를 생성하게 된다.The taste of brewed soy sauce is produced during fermentation and ripening. Starch is broken down into sugars by the enzymatic action secreted by the bacteria, and the protein is broken down into peptides and amino acids by the enzyme action. At the same time, a small amount of alcohol and carbonic acid gas are generated while the fermentation effect is gradually caused by yeasts related to the soy sauce, and organic acids are involved in taste.

양조간장의 색은 메일라이드 반응으로 형성되며, 향은 에스테르화 반응등으로 생성된다.The color of the brewed soy sauce is formed by the Maillide reaction, and the scent is produced by the esterification reaction.

산업적으로 대량 생산되는 양조간장의 주원료인 탈지대두는 종국을 접종하기 전에 잡균의 오염을 막고 발효, 숙성과정에서 효소반응에 의해 분해가 잘 일어나도록 하기 위해 가열, 증자과정을 거치게 된다. 이러한 전통적인 가열, 증자과정 대신에 익스트루더로 탈지대두를 처리하면 압출성형(extusion)과정에서 고온, 고압으로 인해 살균과 동시에 탈재대두중의 단백질과 탄수화물의 변성과 호화가 더 잘 일어나 간장 발효 및 숙성공정에서 수율을 증대시키고 발효시간도 단축될 수 있다. 또한 스팀에 의한 가열, 증자과정을 익스트루더로 대체함으로써 에너지를 절감하고 공정의 단순화로 생산성을 높일 수 있는 이점도 있다.Defatted soybean, the main raw material of industrially produced brewing soy sauce, is heated and steamed to prevent contamination of various germs and to be degraded by enzyme reaction during fermentation and ripening before inoculation. Instead of the traditional heating and steaming process, degreasing soybean treatment with extruder causes sterilization due to high temperature and high pressure during extrusion process, and degeneration and gelatinization of protein and carbohydrate in degreased soybean are more effective. In the aging process, the yield can be increased and the fermentation time can be shortened. In addition, by replacing the steam heating and steaming process with an extruder, there is an advantage to save energy and increase productivity by simplifying the process.

또한, 이러한 압출성형 전처리 과정에서 탈지대두에 효모 추출물(Yeast extract)이나 또는 효소나 산에 의한 식물성 단백 가수분해물(HVP)과 같은 질소원을 첨가하게 되면 효모 추출물 및 식물성 단백 가수분해물중의 펩타이드 및 아미노산이 코지(KOJI) 제조시에 질소원으로 이용되어 종균의 성장 및 효소 생성을 증가시키게 되어, 발효 효율을 한층 증가시킬 수 있었다.In addition, if a nitrogen source such as yeast extract or yeast extract or vegetable protein hydrolysate (HVP) by enzyme or acid is added to the defatted soybean during the extrusion pretreatment, peptides and amino acids in yeast extract and vegetable protein hydrolyzate It was used as a nitrogen source in the manufacture of KOJI to increase the growth of the spawn and the production of enzymes, which further increased fermentation efficiency.

본 발명의 구체적인 가공공정은 다음과 같다.The specific processing process of this invention is as follows.

본 발명에서 익스트루더의 조작은 일정량의 효모 추출물, 또는 식물성 단백 가수분해물과 탈지대두의 혼합물을 용적식 원료 투입기를 사용하여 일정한 속도로 투입하였으며, 수분은 액체 주입 노즐(liquid inletnozzle)을 통하여 주입함으로써 함량을 일정하게 유지하였다. 익스트루더는 1축 또는 2축의 형태로서 익스트루더 운전중 스크루우 회전속도, 원료투입량, 가수량, 압출온도 및 압력, 스크루우 비틀림 등을 일정한 시간 간격으로 컴퓨터에 의해 자동측정하여 기록하였다.Operation of the extruder in the present invention was a constant amount of yeast extract, or a mixture of vegetable protein hydrolyzate and skim soybeans was introduced at a constant rate using a volumetric feeder, the water is injected through a liquid inletnozzle (liquid inletnozzle) Thereby keeping the content constant. The extruder is a uniaxial or biaxial type, and the screw rotation speed, raw material input, water content, extrusion temperature and pressure, screw twist, etc. during the extruder operation were automatically measured and recorded by a computer at regular intervals.

압출성형 조건은 효모 추출물, 또는 식물성 단백 가수분해물 함량(건조 무게비), 바렐온도, 스크루우 회전속도, 초기 수분함량을 독립 변수로하여 반응표면분석법(Response Surface Methodology)에 의해 최적화 시켰다. 반응표면분석법에 의해 얻어진 익스트루더 처리조건들은 압출성형물들의 제국 및 양조간장 제조원료로서 적합한 물리화학적 특성에 대하여 매우 유의성 있는 영향을 보여 주었다.Extrusion conditions were optimized by Response Surface Methodology using yeast extract or vegetable protein hydrolyzate content (dry weight ratio), barrel temperature, screw speed, and initial water content as independent variables. The extruder treatment conditions obtained by reaction surface analysis showed a very significant effect on the physicochemical properties of the extruded moldings as an imperial and brewed soy sauce.

[실시예 1]Example 1

효모 추출물 0.1%가 함유된 탈지대두를 단축 익스트루더를 사용하여 압출성형하였다. 이때 익스트루더 처리조건은 바렐온도 156℃, 초기수분함량 28%, 그리고 스크루우 회전속도 280rpm이었다.Degreased soybean containing 0.1% of yeast extract was extruded using a uniaxial extruder. The extruder treatment conditions were barrel temperature 156 ℃, initial moisture content 28%, and screw rotation speed 280rpm.

이와같이 제조한 압출성형물로 제국(Koji제조)실험 및 간장 숙성실험을 실시하였다. 코지는 압출성형물과 볶아서 파쇄한 적소맥을 같은 양으로 혼합하여 수분함량이 44%가 되도록 가수한 다음, 종국(Aspergillus oryzae)을 0.04% 접종하여 가락 형태로 만들어 제국상자에 일정량씩 담아 배양기에서 27-30℃, 상대습도 90%에서 72시간 배양하였다. 간장은 상기 코지를 사용하여 통상적인 방법으로 제조하여 6개월간 숙성시킴으로써 만들어졌다.The extruded moldings thus prepared were subjected to the Imperial (Koji) test and the soy sauce ripening test. Koji mixes the extruded product with roasted crushed red wheat flour in the same amount, adds water to 44%, and inoculates 0.04% of Aspergillus oryzae to form a rhythm. It was incubated for 72 hours at -30 ° C and 90% relative humidity. Soy sauce was prepared by conventional methods using the koji and aged for 6 months.

대조구로서 효모 추출물을 첨가하지 않은 압출성형물을 코지 제조 원료로 사용하였다.As a control, an extruded product to which the yeast extract was not added was used as a raw material for manufacturing the paper.

[실시예 2]Example 2

효모 추출물의 함량을 실시예 1과 다르게 변화시키면서 표 1의 조건으로 압출성형물을 제조하였다.Extrusions were prepared under the conditions of Table 1 while changing the content of the yeast extract from Example 1.

이하 실시예 1의 방법과 동일한 조건으로 간장 제조 및 숙성 실험을 하여 그 결과를 표 2에 나타내었다.Soy sauce was prepared and aged under the same conditions as in Example 1, and the results are shown in Table 2.

[표 1]TABLE 1

[표 2]TABLE 2

* : 기존의 압출성형물을 원료로 제조한 코지의 자기소화율을 100%로 한 비교치*: Comparison value of 100% of self extinguishing rate of Cozy made from existing extruded materials

** : 기존의 압출성형물을 원료로 제조한 간장의 총질소함량을 100%로 한 비교치**: Comparison value of total nitrogen content of soy sauce made from existing extruded raw materials as 100%

코지 자기소화 시험 결과 기존의 압출성형물로 제조한 코지와 비교하여 효모 추출물을 첨가한 압출성형물로 제조한 코지에서 15-25% 이상 높은 자기소화율을 나타냈다. 간장 숙성 실험결과 6개월 후 총질소함량은 기존의 압출성형물로 제조한 간장과 비교하여 효모 추출물을 첨가한 압출성형물로 제조한 간장이 9-24% 정도 높았다.Cozy self-extinguishing test resulted in a higher self-extinguishing rate of 15-25% or more in the coge prepared with the yeast extract added as compared with the coge prepared with the conventional extrudate. After 6 months, the total nitrogen content was 9-24% higher than that of the extruded products with yeast extract compared to the conventional extruded soy sauce.

[실시예 7]Example 7

효모 추출물 대신 단백 효소 분해물 0.1%가 함유된 탈지대두를 2축 익스트루더를 사용하여 압출성형하였다. 이때 익스트루더 처리조건은 바렐온더 156℃, 초기수분함량 28%, 그리고 스크루우 회전속도 280rpm이었다.Degreased soybean containing 0.1% protein degradant instead of yeast extract was extruded using a biaxial extruder. The extruder treatment conditions were barrel onder 156 ℃, initial moisture content 28%, and screw rotation speed 280rpm.

[실시예 8-12]Example 8-12

단백 효소 분해물의 함량을 실시예 7과 다르게 변화시키면서 표3의 조건으로 압출성형물을 제조하였다.Extruded products were prepared under the conditions of Table 3 while varying the content of protein enzyme digests from Example 7.

이하 실시예 1의 방법과 동일한 조건으로 간장 제조 및 숙성 실험을 하여 그 결과를 표 4에 나타내었다.Soy sauce was prepared and aged under the same conditions as in Example 1, and the results are shown in Table 4.

[표 3]TABLE 3

[표 4]TABLE 4

* : 기존의 압출성형물을 원료로 제조한 코지의 자기소화율을 100%로 한 비교치*: Comparison value of 100% of self extinguishing rate of Cozy made from existing extruded materials

** : 기존의 압출성형물을 원료로 제조한 간장의 총질소함량을 100%로 한 비교치**: Comparison value of total nitrogen content of soy sauce made from existing extruded raw materials as 100%

코지 자기소화 시험결과 기존의 압출성형물로 제조한 코지와 비교하여 단백 효소 분해물을 첨가한 압출성형물로 제조한 코지에서 19-27% 이상 높은 자기소화율을 나타냈다. 간장 숙성 실험결과 6개월 후 총질소함량은 기존의 압출성형물로 제조한 간장과 비교하여 단백 효소물을 첨가한 압출성형물로 제조한 간장이 15-26% 정도 높았다.Cozy self-extinguishing test showed 19-27% higher self-extinguishing rate in Cozy made with Extruded Additives than Protein Enzyme. After 6 months, the total nitrogen content was 15-26% higher than that of the extruded product containing protein enzyme, compared to the soy prepared by the conventional extruded product.

[실시예 13]Example 13

단백 산가수 분해물 0.1%가 함유된 탈지대두를 1축 익스트루더를 사용하여 압출성형하였다. 이때 익스트루더 처리조건은 바렐온도 146℃, 초기 수분함량 20%, 그리고 스크루우 회전속도 280rpm이었다.Defatted soybean containing 0.1% protein acid hydrolyzate was extruded using a single screw extruder. The extruder treatment conditions were barrel temperature 146 ℃, initial moisture content 20%, and screw rotation speed 280rpm.

[실시예 14-18]Example 14-18

단백 산가수분해물의 함량을 실시예 13과 다르게 변화시키면서 표 5의 조건으로 압출성형물을 제조하였다.Extrusions were prepared under the conditions of Table 5 while varying the content of protein acid hydrolyzate from Example 13.

이하 실시예 1의 방법과 동일한 조건으로 간장 제조 및 숙성 실험을 하여 그 결과를 표 6에 나타내었다.Soy sauce was prepared and aged under the same conditions as in Example 1, and the results are shown in Table 6.

[표 5]TABLE 5

[표 6]TABLE 6

* : 기존의 압출성형물을 원료로 제조한 코지의 자기소화율을 100%로 한 비교치*: Comparison value of 100% of self extinguishing rate of Cozy made from existing extruded materials

** : 기존의 압출성형물을 원료로 제조한 간장의 총질소함량을 100%로 한 비교치**: Comparison value of total nitrogen content of soy sauce made from existing extruded raw materials as 100%

코지 자기소화 실험결과 기존의 압출성형물로 제조한 코지와 비교하여 단백 산가수분해물을 첨가한 압출성형물로 제조한 코지에서 19-25% 이상 높은 자기소화율을 나타냈다. 간장 숙성 시험결과가 6개월후 총질소함량은 기존의 압출성형물로 제조한 간장과 비교하여 효모 추출물을 첨가한 압출성형물로 제조한 간장이 14-25% 정도 높았다.Cozy self-extinguishing experiments showed a higher self-extinguishing rate of more than 19-25% in the cozy made with the extruded product to which the protein acid hydrolyzate was added. After 6 months, the total nitrogen content of the soy sauce ripening test was 14-25% higher than that of the conventional extruded soy sauce.

단백 분해물을 첨가한 압출성형물을 이용하여 제조한 간장의 품질을 평가하기 위하여 기존의 압출성형물로 제조한 간장과 함께 실시한 관능검사 결과는 표 7과 같다.In order to evaluate the quality of the soy sauce prepared using the extruded product to which the protein decomposition product was added, the sensory test results performed with the soy sauce prepared with the existing extruded products are shown in Table 7.

[표 7]TABLE 7

[표 8]TABLE 8

[표 9]TABLE 9

관능검사 : 9월 기호 척도법으로 실시, 관능검사 요원은 실험과 무관한 20명Sensory evaluation: Sept., conducted by symbolic scale method. Sensory evaluation personnel were 20 persons who were not related to the experiment.

a : 5%의 유의 수준으로 유의차 없음a: 5% significance level, no significant difference

b : 5%의 유의 수준으로 유의차 없음b: No significant difference at 5% significance level

표 7, 표 8, 표 9에서 볼 수 있듯이 압출성형 추출물 및 단백 가수분해물을 첨가한 압출성형물로 제조한 간장은 첨가하지 않은 압출성형물로 제조한 간장과 비교하여 전체적인 기호도 및 향에서 유의적인 차이를 나타내지 않아 첨가한 단백 분해물이 간장 발효에 의한 향 및 맛에 영향을 미치지 않는 것을 알 수 있다. 따라서 효모 추출물 및 단백 가수분해물을 첨가하여 탈지대두를 익스트루더로 처리하면 산업적으로 양조간장을 제조할 때 단백 분해물에 의해 종균의 성장 및 효소 생성을 촉진시켜 간장 제조 수율이 높아지는 효과가 있는 것을 알 수 있다.As can be seen from Table 7, Table 8 and Table 9, the soy sauce prepared with the extruded extract with the extruded extract and the protein hydrolyzate showed a significant difference in overall preference and aroma compared with the soy prepared with the extruded extract without the added extract. It can be seen that the added protein degradation product does not affect the aroma and taste by soybean fermentation. Therefore, if yeast extract and protein hydrolyzate are added to treat skim soybean with an extruder, it is found that the yield of soy sauce is increased by promoting the growth of enzymes and the production of enzymes by protein degradation when brewing soy sauce is industrially produced. Can be.

Claims (1)

효모 추출물 0.05-1%나 또는 식물성 단백 가수분해물 0.05-1.5%가 첨가된 탈지대두를 1축 또는 2축 익스트루더를 사용하여 바렐온도 110-190℃, 초기 수분함량 10-50%, 스크루우 회전속도 250-400rpm의 조건에서 압출성형함을 특징으로 하는 양조 간장용 원료의 제조방법.Defatted soybean containing 0.05-1% yeast extract or 0.05-1.5% of vegetable protein hydrolyzate was subjected to barrel temperature 110-190 ℃, initial moisture content 10-50%, screw Method for producing a soy sauce raw material for brewing, characterized in that the extrusion molding under the conditions of the rotation speed 250-400rpm.
KR1019910025892A 1991-12-31 1991-12-31 Process for making raw of soysauce by extruder KR940002531B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005007749A1 (en) * 2003-07-11 2005-01-27 Mgp Ingredients, Inc. Grain protein-based formulations and methods of using same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005007749A1 (en) * 2003-07-11 2005-01-27 Mgp Ingredients, Inc. Grain protein-based formulations and methods of using same

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