KR930023468A - L-phenylalanine recovery method using membrane separation - Google Patents

L-phenylalanine recovery method using membrane separation Download PDF

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Publication number
KR930023468A
KR930023468A KR1019920007678A KR920007678A KR930023468A KR 930023468 A KR930023468 A KR 930023468A KR 1019920007678 A KR1019920007678 A KR 1019920007678A KR 920007678 A KR920007678 A KR 920007678A KR 930023468 A KR930023468 A KR 930023468A
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South Korea
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amino acid
emulsion
reaction
fermentation broth
liquid film
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KR1019920007678A
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Korean (ko)
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홍성안
허창기
김종선
정병태
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박원희
한국과학기술연구원
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Publication of KR930023468A publication Critical patent/KR930023468A/en

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Abstract

본 발명은 발효액으로부터 특정 아미노산을 유화액막 추출법을 이용하여 선택적으로 추출ㆍ농축하여 회수하는 공법에 있어서, 전처리를 거친 발효액의 pH를 아미노산의 등전점 이하로 낮춘 다음, 유화액막과 접촉시켜 인산기를 갖는 음이온성 담체를 이용해서 양이온화된 아미노산을 선택적으로 추출하고, 전기적 방법으로 유화액막을 파괴하여 얻어진 내부 수용액상으로 아미노산을 중화법으로 회수하는 방법에 관한 것이다.In the process of selectively extracting and concentrating a specific amino acid from the fermentation broth using an emulsion film extraction method, the present invention lowers the pH of the fermentation broth after pretreatment to below the isoelectric point of the amino acid, and then contacts the emulsion film to form an anion having a phosphate group. The present invention relates to a method for selectively extracting a cationized amino acid using a sex carrier and recovering the amino acid by neutralization into an internal aqueous solution obtained by breaking the emulsion film by an electrical method.

Description

막분리를 이용한 L-페닐알라닌 회수방법L-phenylalanine recovery method using membrane separation

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

아미노산 발효액에서 아미노산을 회수하기 위하여 발효액에 함유된 균체 및 불순물을 제거한 액에 액막과 강산을 혼합해 만든 유화액을 혼합하여 반응시킨 후, 유화 액막층을 해유화하여 해유화된 액막층을 재사용하고 아미노산을 회수하는 방법.In order to recover the amino acid from the amino acid fermentation broth, the solution which removes the cells and impurities contained in the fermentation broth is mixed and reacted with an emulsion made by mixing a liquid film and a strong acid, and then the emulsion liquid film layer is demulsified to reuse the demulsified liquid film layer. How to recover. 제1항에 있어서, 발효액에 강산을 사용하며 pH1.0-4.0, 80-150℃, 10-20분간 반응한 후, 규조토를 사용해서 불순물을 제거하고 여액을 강산으로 pH2.0-5.0으로 조절함을 특징으로 하는 아미노산 회수 방법.According to claim 1, using a strong acid in the fermentation broth, pH1.0-4.0, 80-150 ℃, 10-20 minutes after the reaction, using a diatomaceous earth to remove impurities and the filtrate adjusted to pH2.0-5.0 with a strong acid Amino acid recovery method characterized in that. 제1항에 있어서, 아미노산이 페닐알라닌 임을 특징으로 하는 아미노산 회수 방법.The method of claim 1, wherein the amino acid is phenylalanine. 제1항에 있어서, 아미노산이 트립토판 임을 특징으로 하는 아미노산 회수 방법.The method of claim 1 wherein the amino acid is tryptophan. 제1항에 있어서, 액막 추출의 효율을 극대화 시키기 위하여 발효액과 유화막의 반응비를 3 : 1-4 : 1로 하고, 반응 직전 발효액의 pH를 2.0-5.0, 아미노산 농도를 3.0-4.0%로 하고, 25-50℃에서 반응시킴을 특징으로 하는 아미노산 회수 방법.The method according to claim 1, wherein in order to maximize the efficiency of liquid membrane extraction, the reaction ratio between the fermentation broth and the emulsion membrane is 3: 1-4: 1, and the pH of the fermentation broth immediately before the reaction is 2.0-5.0 and the amino acid concentration is 3.0-4.0%. , Amino acid recovery method characterized in that the reaction at 25-50 ℃. 제1항에 있어서, 아미노산의 회수율을 극대화 시키기 위하여 1단 추출반응과 3단 추출 반응을 병렬 접속시켜 사용하며, 전자의 경우 발효액과 에멀젼을 3 : 1로 반응시키고, 후자는 4 : 1로 반응시킴을 특징으로 하는 아미노산 회수 방법.According to claim 1, in order to maximize the recovery of amino acid is used in parallel connection of one-stage extraction reaction and three-stage extraction reaction, the former reaction of the fermentation broth and the emulsion in a 3: 1, the latter is a 4: 1 Amino acid recovery method characterized in that. 제1항에 있어서, 액막은 점도 7.5cp(40℃)인 오일과 인산의 알킬 유도체, 그리고 계면활성제를 사용하여 만듬을 특징으로 하는 아미노산 회수 방법.The method of claim 1, wherein the liquid film is made using an alkyl derivative of oil and phosphoric acid having a viscosity of 7.5 cp (40 DEG C), and a surfactant. 제1항에 있어서, 해유화 하는 방법으로 고압, 고주파 전류를 사용하여 유화 액막층을 해유화 시킴을 특징으로 하는 아미노산 회수 방법.The amino acid recovery method according to claim 1, wherein the emulsion liquid film layer is demulsified using a high pressure and a high frequency current as a demulsification method. 제3항에 있어서, 오일과 인산의 알킬유도체, 계면활성제에 대하여 S60NR, D2EHPA, Px100을 각각 사용함을 특징으로 하는 아미노산 회수 방법.4. A method for recovering amino acids according to claim 3, wherein S60NR, D2EHPA, and Px100 are used for the alkyl derivatives of the oil and phosphoric acid and the surfactant, respectively. 제3항에 있어서, 액막에 강산을 투입하여 유탁(emulsify)시켜 에멀젼을 만들고, 추출 반응 이후 유화 액막층을 분리함을 특징으로 하는 아미노산 회수 방법.The amino acid recovery method according to claim 3, wherein a strong acid is added to the liquid film to emulsify the emulsion, and an emulsion liquid film layer is separated after the extraction reaction. ※참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is to be disclosed based on the initial application.
KR1019920007678A 1992-05-07 L-phenylalanine recovery method using membrane separation KR930023468A (en)

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KR930023468A true KR930023468A (en) 1993-12-18

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