KR920703100A - Specific DNA Sequences, Vectors, Hosts, and Vaccines Associated with IBDV Proteins - Google Patents

Abstract

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Description

Specific DNA Sequences, Vectors, Hosts, and Vaccines Associated with IBDV Proteins

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Claims (26)

Biologically pure, characterized in that it comprises an RNA sequence encoding at least one copy of a polypeptide having antibody binding properties of lBOV VP2 protein derived from at least one US variant selected from the group consisting of E / DEL and CLS strains RNA fragments. The method of claim 1. An RNA fragment in which the encoded polypeptide comprises the antibody binding properties of amino acids 200 to 330 of the VP2 protein. The RNA fragment of claim 1, comprising about 3.2K bases. 2. The RNA sequence of claim 1, wherein the RNA sequence has an amino acid sequence of Tables 6 and 7, having at least one homologue, di-functional, amino acid sequence of at least one homologue at its position consisting of the amino acid sequence of 5,74,84,213,222,239,249,253,254,258,264,269,270,272,279,280,284,286,297,299,305,318,321,323,326,328,330,332 and 433. An RNA fragment encoding a polypeptide fragment having one amino acid sequence selected from the group consisting of sex precursors and mixtures thereof. The RNA sequence of claim 4, wherein the RNA sequence comprises an RNA sequence encoding VP2, VP3, and VP4 proteins of GLS and E / DEL IBDV. A biologically pure DNA fragment, characterized in that it comprises a single stranded DNA sequence corresponding to the RNA fragment of claim 1. The DNA fragment of claim 6, which is in double strand form. 7. The DNA fragment of claim 6 wherein the DNA sequence comprises the DNA sequences of Tables 6, 7 and 8. A recombinant vector comprising a vector capable of growing and expressing a structural DNA sequence bound in a host, and at least one copy of the DNA fragment of claim 7 bound to a vector in a read frame. The recombinant vector of claim 9, wherein the vector comprises recombinant infectious epithelial tumor (fowl pox virus or herpes virus from turkey (HVT). The additional DNA sequence of claim 9, which encodes at least one polypeptide that exhibits protection against a 'Classic' US IBDV variant or an Australian, German or European IBDV strain, the DNA sequence being read into a vector. Recombined in a frame). An IBDV poultry vaccine having a broad spectrum, characterized by containing a protective amount of poultry, the recombinant vector of claim 9 and a physiologically acceptable carrier. 13. A wide range vaccine according to claim 12, containing about 10 ⁴ to 10 ⁷ pfu units of recombinant vector per ml carrier. An IBDV poultry vaccine having a broad spectrum, characterized by containing a protected poultry amount of the biologically pure DNA fragment of claim 7 and a physiologically acceptable carrier. 15. The IBDV poultry vaccine according to claim 14, which contains a recombinant vector of about 10 ⁴ to 10 ⁷ pfu units per ml carrier. The host of claim 9 having a recombinant vector. The method of claim 16, wherein: E. coli, insect strain Sf-9, chick embryo fibroblast (CEF) chick embryo kidney (CEK) cells and vero cells. A biologically pure polypeptide, characterized in that it comprises at least one copy of an amino acid sequence encoded by the RNA fragment of claim 1. The amino acid sequence of claim 18, wherein the amino acid sequence is of amino acids of Tables 6, 7, and 8, 5, 74, 84, 213, 222, 239, 249, 253, 254, 258, 264, 269, 270, 272, 279, 280 , Homologues thereof having different amino acids at least one position selected from the group consisting of 284, 286, 297, 299, 305, 318, 321, 323, 326, 328, 330, 332 and 433, functional fragments thereof, A polypeptide comprising one sequence selected from the group consisting of functional precursors and mixtures thereof. A method of protecting poultry and its descendants from IBD, comprising administering to the poultry the recombinant vector of claim 9 in an amount effective to achieve the desired effect. The method of claim 20, wherein the recombinant vector is orally administered to the subject via injection, nasal or oral. The method of claim 20, wherein the subject is administered a recombinant vector in an amount of about 10 ² to 10 ⁸ pfu. A method of protecting poultry and its offspring from IBD, comprising administering to the poultry the biologically pure DNA fragment of claim 7 in an amount effective to achieve the desired effect. The method of claim 23, wherein the DNA is orally administered to the subject via injection, nasal or oral. The method of claim 23, wherein the DNA is administered to the subject in an amount of about 10 ² to about 10 ⁶ pfu. ※ Note: The disclosure is based on the initial application.