KR920701243A - T-lymphoid retroviral monoclonal antibody - Google Patents

Abstract

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Description

T-lymphoid retroviral monoclonal antibody

Since this is an open matter, no full text was included.

1 is a graph showing the reactivity of the culture supernatants by the capture ELISA method, detailed description is shown in Table 1. 2 is a photograph of an immunoblot nitro cellulose strip measuring the specificity of anti-HIV antibodies. Figure 3 uses protein A-purified antibodies as probes to HIV-2 protein isolated from immune blots. Lanes 1 and 2 are for positive control and lane 3 is for negative control.

Claims (33)

And a epitope of p24 of HIV-1 and an epitope of p26 of HIV-2, wherein the epitope is located within amino acid residues 140-160 of p24. The monoclonal antibody of claim 1, wherein the epitope is located within amino acid residues 142-145 of p24. The monoclonal antibody of claim 1, wherein the epitope is located within amino acid residues 144-148 of p24. The monoclonal antibody of claim 1, wherein the epitope comprises the amino acid sequence Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys. The epitope of claim 1, wherein the epitope comprises the amino acid sequence His-XXX-Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys-X, wherein X is any biologically consistent Monoclonal antibody, characterized in that the amino acid. Reacts with an antigen comprising the amino acid sequence of His-XXX-Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys-X, wherein X is any amino acid with biological activity Characterized in a monoclonal antibody. Monoclonal antibody that reacts with an antigen consisting of the amino acid sequence Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys. Consisting of the amino acid sequence His-XXX-Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys-X, wherein the monoclonal antibody of claim 1 is any amino acid of biological activity Reactive epitopes. An epitope comprising the amino acid sequence Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys that reacts with the monoclonal antibody of claim 1. Amino acid sequence of His-X-X-X-X-Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys-X, wherein X is any amino acid of matching biological function. The amino acid sequence is Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys. A diagnostic kit for the detection of HIV-1 and HIV-2 containing at least one antibody that reacts with an antigen of HIV-1 and the monoclonal antibody of claim 1. The diagnostic kit of claim 12, wherein the antibody is a monoclonal antibody. The diagnostic kit of claim 13, wherein the epitope of the antibody comprises the amino acid sequence Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys. The diagnostic kit of claim 14, comprising two monoclonal antibodies that react with the antigen of HIV-1. 16. The monoclonal antibody of claim 15, wherein one of said monoclonal antibodies that react with an antigen of HIV-1 binds to an epitope located within amino acid residues 142-209 of p24 and reacts with the antigen of HIV-1. A diagnostic kit, wherein the second antibody binds an epitope located within amino acid residues 263-344 of p24. A diagnostic kit for the detection of HIV-1 and HIV-2 containing at least one antibody that reacts with an antigen of HIV-1 and the monoclonal antibody of claim 6. A diagnostic kit for the detection of HIV-1 and HIV-2 containing at least one antibody that reacts with an antigen of HIV-1 and the monoclonal antibody of claim 7. Detection of HIV-1 and HIV-2 antigens in the sample consisting of contacting the sample with at least one antibody and the monoclonal antibody of claim 1 and measuring the formation of antigen-antibody complexes Way. The method of claim 19, wherein the antibody is a monoclonal antibody. The method of claim 20, wherein the epitope comprises an amino acid sequence of Ser-Pro-Arg-Thr-Leu-Asn-Ala-Trp-Val-Lys. The method of claim 21, comprising two monoclonal antibodies that react with the reduction of HIV-1. The monoclonal antibody of claim 22, wherein one of the monoclonal antibodies that react with the antigen of HIV-1 reacts with an epitope located within amino acid residues 142-209 of p24 and the monoclonal antibody that reacts with an antigen of HIV-1. The second antibody reacts with an epitope located within amino acid residues 263-344 of p24. Detecting the detection of HIV-1 and HIV-2 antigens in a sample consisting of contacting the sample with at least one antibody and the monoclonal antibody of claim 6 and measuring the formation of the antigen-antibody complex. Way. A method of detecting HIV-1 and HIV-2 antigens in a sample consisting of contacting the sample with at least one antibody and the monoclonal antibody of claim 7 and measuring the formation of the antigen-antibody complexes. . A method for detecting HIV-1 and HIV-2 antibodies in a sample comprising contacting the epitope of claim 8 with a sample and measuring the formation of an antigen-antibody complex. A method for detecting HIV-1 and HIV-2 antibodies in a sample, comprising contacting the epitope of claim 9 with a sample and measuring the formation of an antigen-antibody complex. A method for detecting HIV-1 and HIV-2 antibodies in a sample comprising contacting a sample with the amino acid sequence of claim 10 and measuring formation of an antigen-antibody complex. A method for detecting HIV-1 and HIV-2 antibodies in a sample comprising contacting a sample with the amino acid sequence of claim 11 and measuring the formation of an antigen-antibody complex. A diagnostic kit for the detection of HIV-1 and HIV-2 antibodies in a sample containing the epitope of claim 8. A diagnostic kit for the detection of HIV-1 and HIV-2 antibodies in a sample containing the epitope of claim 8. A diagnostic kit for detecting HIV-1 and HIV-2 antibodies in a sample, comprising the amino acid sequence of claim 10. A diagnostic kit for detecting HIV-1 and HIV-2 antibodies in a sample, comprising the amino acid sequence of claim 10. ※ Note: This is to be disclosed by the original application.