KR920700402A - Compositions, test kits and assays using the same, containing labeled Streptococcus antibodies - Google Patents

Compositions, test kits and assays using the same, containing labeled Streptococcus antibodies

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Publication number
KR920700402A
KR920700402A KR1019900702355A KR900702355A KR920700402A KR 920700402 A KR920700402 A KR 920700402A KR 1019900702355 A KR1019900702355 A KR 1019900702355A KR 900702355 A KR900702355 A KR 900702355A KR 920700402 A KR920700402 A KR 920700402A
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KR
South Korea
Prior art keywords
labeled
enzyme
antigen
composition
antibody
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KR1019900702355A
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Korean (ko)
Inventor
체스터 해롤드 3세 워렌
개리 루이스 스노드그래스
Original Assignee
존 디. 후써
이스트만 코닥 컴퍼니
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Application filed by 존 디. 후써, 이스트만 코닥 컴퍼니 filed Critical 존 디. 후써
Publication of KR920700402A publication Critical patent/KR920700402A/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56944—Streptococcus

Abstract

내용 없음No content

Description

라벨링된 연쇄상구균의 항체를 함유한 조성물, 테스트 키트 및 이것을 이용한 분석법Compositions, test kits and assays using the same, containing labeled Streptococcus antibodies

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (20)

검출가능하도록 라벨링된 연쇄상구균 항원에 대한 항체 및 수용성이거나 수분산성인 비이온성 계면활성제를 포함하는 수성의 면역학적 조성물.An aqueous immunological composition comprising an antibody against a streptococcal antigen labeled to be detectable and a water soluble or water dispersible nonionic surfactant. 제1항에 있어서, 상기 항체가 방사성 동위원소 또는 효소로 라벨링되는 조성물.The composition of claim 1, wherein said antibody is labeled with a radioisotope or enzyme. 제2항에 있어서, 상기 항체가 효소로 라벨링되는 조성물.The composition of claim 2, wherein the antibody is labeled with an enzyme. 제1항에 있어서, 상기 항체가 연쇄상구균 A항원에 대한 것이고, 퍼옥시다제로 라벨링되는 조성물.The composition of claim 1, wherein the antibody is against Streptococcus A antigen and labeled with peroxidase. 제1항에 있어서, 상기 비이온성 계면활성제가 최소한 약 0.05중량%양으로 존재하는 조성물.The composition of claim 1, wherein the nonionic surfactant is present in an amount of at least about 0.05% by weight. 제1항에 있어서, 상기 비이온성 계면활성제가 폴리옥시에틸렌 에테르, 폴리옥시에틸렌소르비탄 유도체 및 폴리글리콜 에테르로 구성된 그룹으로 부터 선택되는 조성물.The composition of claim 1 wherein said nonionic surfactant is selected from the group consisting of polyoxyethylene ethers, polyoxyethylene sorbitan derivatives and polyglycol ethers. 제6항에 있어서, 상기 비이온성 계면활성제가 폴리옥시에틸렌 소르비탄 유도체인 조성물.7. The composition of claim 6, wherein said nonionic surfactant is a polyoxyethylene sorbitan derivative. 제7항에 있어서, 상기 비이온성 계면활성제가 폴리옥시에틸렌 소르비탄 모노라우레이트인 조성물.8. The composition of claim 7, wherein said nonionic surfactant is polyoxyethylene sorbitan monolaurate. 제1항에 있어서, 완충제, 수용성 단백이나 탄수화물 및 페놀을 더 포함하는 조성물.The composition of claim 1 further comprising a buffer, water soluble protein or carbohydrate, and phenol. a. 연쇄상구균으로 부터 연쇄상구균 항원을 추출하기 위한 하나 이상의 시약 및,a. One or more reagents for extracting streptococcal antigen from streptococci, and b. 검출가능하도록 라벨링된 상기 연쇄상구균 항원에 대한 항체와 수용성이거나 수분산성인 비이온성 계면활성제를 포함하는 수성의 면역학적 조성물로 구성되는, 연쇄상구균을 검출하는데 유용한 면역학적 진단 테스트 키트.b. An immunological diagnostic test kit useful for detecting streptococci, comprising an aqueous immunological composition comprising an antibody to the streptococcal antigen labeled detectably and a water soluble or water dispersible nonionic surfactant. 제10항에 있어서, 상기 항체가 효소로 라벨링되고 상기 키트가 상기 효소의 존재에서 검출가능한 종류를 제공하는 하나 이상의 시약을 더 포함하는 테스트 키트.The test kit of claim 10, wherein the antibody is labeled with an enzyme and the kit further comprises one or more reagents that provide a detectable species in the presence of the enzyme. a. 효소로 라벨링된 연쇄상구균 항원에 대한 항체와 수용성이거나 수분산성인 비이온성 계면활성제를 포함하는 수성의 면역학적 조성물, 및a. An aqueous immunological composition comprising an antibody to an Streptococcus antigen labeled with an enzyme and a nonionic surfactant that is water soluble or water dispersible, and b. 상기 효소의 존재에서 검출가능한 종류를 제공하는 하나 이상의 시약으로 구성되는, 연쇄상구균을 검출하는데 유용한 면역학적 진단 테스트 키트.b. An immunological diagnostic test kit useful for detecting streptococci, comprising one or more reagents that provide a detectable species in the presence of the enzyme. 제12항에 있어서, 상기 연쇄상구균 항원에 대한 라벨링되지 않은 항체를 고정화시킨 일회용 테스트 장치를 더 포함하는 데스트 키트.The test kit of claim 12, further comprising a disposable test device immobilized with unlabeled antibodies to the streptococcal antigen. 제12항에 있어서, 상기 효소가 퍼옥시다제이고 상기 하나이상의 시약이 퍼옥시다제 및 과산화수소의 존재에서 염료를 제공하는 트리아릴이미다졸 루코 염료를 포함하는 테스트 키트.The test kit of claim 12, wherein said enzyme is a peroxidase and said at least one reagent comprises a triarylimidazole leuco dye that provides a dye in the presence of peroxidase and hydrogen peroxide. A. 추출된 연쇄상구균 항원을 함유하고 있는 샘플을 검출가능하도록 라벨링한 상기 연쇄상구균 항원에 대한 항체 및 수용성이거나 수분 산성인 비이온성 계면활성제로 구성된 수성의 면역학적 조성물과 접촉시켜 검출가능하도록 라벨링된 면역학적 복합체를 형성시키고,A. Detectable labeled by contacting with an aqueous immunological composition consisting of an antibody against the streptococcal antigen labeled with a detectable labeled streptococcal antigen and a water soluble or water acidic nonionic surfactant. To form an immunological complex, B. 상기 샘플내 항원의 존재 표시로서 상기 복합체의 존재를 검출하는 단게로 구성되는 연쇄상구균의 검출방법.B. A method for detecting streptococci, comprising the step of detecting the presence of the complex as an indication of the presence of antigen in the sample. 제15항에 있어서, 상기 추출된 항원이 단계 A전과 추출후에 이러한 항원에 대한 고정화되고 라벨링되지 않은 항체와 접촉되는 방법.The method of claim 15, wherein said extracted antigen is contacted with immobilized, unlabeled antibody to this antigen before and after step A. 17. 제15항에 있어서, 상기 항체가 효소로 라벨링되고 상기 복합체를 검출하는 것이 상기 복합체를 상기 효소의 존재에서 검출가능한 종류를 제공하는 하나 이상의 시약과 접촉시킴에 의해 실행되는 방법.The method of claim 15, wherein the antibody is labeled with an enzyme and the detecting of the complex is performed by contacting the complex with one or more reagents that provide a detectable species in the presence of the enzyme. A. 연쇄상구균 A를 함유하고 있는 샘플을 이러한 미생물로 부터 항원을 추출하기 위한 하나 이상의 추출 시약과 접촉시키고,A. A sample containing Streptococcus A is contacted with one or more extraction reagents to extract antigens from these microorganisms, B. 상기 추출된 연쇄상구균 A항원을 효소로 라벨링한 상기 연쇄상구균 A항원에 대한 항체 및 수용성이거나 수분산성인 비이온성 계면활성제로 구성된 수성의 면역학적 조성물과 접촉시켜 효소로 라벨링된 면역학적 복합체를 형성시키며,B. The enzyme-labeled immunological complex is contacted with an aqueous immunological composition consisting of the antibody against the Streptococcus A antigen labeled with the enzyme and the water-soluble or water-dispersible nonionic surfactant. Form, C. 단계 A후의 단계 B전에, 또는 B와 동시에 또는 연속해서, 상기 추출된 연쇄상구균 A항원을 고정화되거나 고정화될수 있으며 라벨링되지 않은 상기 항원에 대한 항체와 접촉시켜 항원과 라벨링되지 않은 항체와의 복합체를 형성시키고,C. The complex of the extracted Streptococcus A antigen, immobilized or immobilized and contacted with an antibody against the above-labeled antigen, which may be immobilized or immobilized before or after step B after step A, or simultaneously with or after B. Form, D. 라벨링된 항체 및 라벨링되지 않은 항체와 항원과의 결과의 복합체를 효소 존재에서 염료를 제공하는 하나 이상의 시약과 접촉시키며,D. contacting the resulting complex of labeled and unlabeled antibodies with an antigen with one or more reagents that provide a dye in the presence of an enzyme, E. 상기 샘플내 연쇄상구균 A의 존재 표시로서 상기 염료의 존재를 검출하는 단계로 구성되는, 연쇄상구균 A의 검출방법.E. A method for detecting Streptococcus A, comprising detecting the presence of the dye as an indication of the presence of Streptococcus A in the sample. 제18항에 있어서, 상기 효소가 퍼옥시다제이고 상기 염료가 트리아릴이미다졸 루코 염료와 과산화수소를 이용해 형성되는 방법.19. The method of claim 18, wherein said enzyme is a peroxidase and said dye is formed using triarylimidazole leuco dye and hydrogen peroxide. 제18항에 있어서, 상기 비이온성 계면활성제가 최소한 약 0.05중량%으로 존재하며 폴리옥시에틸렌에테르, 폴리옥시에틸렌소트 비탄 유도체 및 폴리글리콜 에테르로 구성된 그룹으로 부터 선택되는 방법.The method of claim 18, wherein the nonionic surfactant is present at least about 0.05% by weight and is selected from the group consisting of polyoxyethylene ether, polyoxyethylene sorted non-derived derivatives, and polyglycol ethers. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900702355A 1989-03-01 1990-02-13 Compositions, test kits and assays using the same, containing labeled Streptococcus antibodies KR920700402A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31738289A 1989-03-01 1989-03-01
US317382 1989-03-01
PCT/US1990/000761 WO1990010232A1 (en) 1989-03-01 1990-02-13 Composition containing labeled streptococcal antibody, test kit and assay using same

Publications (1)

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KR920700402A true KR920700402A (en) 1992-02-19

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Application Number Title Priority Date Filing Date
KR1019900702355A KR920700402A (en) 1989-03-01 1990-02-13 Compositions, test kits and assays using the same, containing labeled Streptococcus antibodies

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EP (1) EP0413810A1 (en)
JP (1) JPH03501653A (en)
KR (1) KR920700402A (en)
CA (1) CA2028146A1 (en)
FI (1) FI905350A0 (en)
WO (1) WO1990010232A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4035174A1 (en) * 1990-11-06 1992-05-07 Biotest Ag METHOD FOR DETERMINING PROTEINS IN BODY LIQUIDS AND MEANS FOR IMPLEMENTING THE METHOD
US5627080A (en) * 1994-07-29 1997-05-06 Beckman Instruments, Inc. Detergent-facilitated immunoassay for the rapid and quantitative assay of pharmacological agents
WO2007092360A2 (en) * 2006-02-02 2007-08-16 Ethicon, Inc. Diagnostics and methods for removal and detection of interferents
EP2739975B1 (en) * 2011-08-03 2015-06-17 Quidel Corporation N-acetyl-d-glucosamine for enhanced specificity of strep a immunoassay
NZ721269A (en) 2013-12-18 2017-09-29 Asahi Chemical Ind Method for detecting streptococcus bacterium contained in milk

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0061541A1 (en) * 1981-03-27 1982-10-06 Biospecia Limited Immunological analysis and a biochemical agent therefor
EP0109012A3 (en) * 1982-11-12 1984-08-08 Abbott Laboratories Determination of streptococci
GB8317855D0 (en) * 1983-06-30 1983-08-03 Iq Bio Ltd Biochemical detection method
US4810630A (en) * 1987-03-30 1989-03-07 E. I. Du Pont De Nemours And Company Use of polyoxyethylene ethers to improve the performance of immunoassays employing peroxidase conjugates

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EP0413810A1 (en) 1991-02-27
CA2028146A1 (en) 1990-09-02
WO1990010232A1 (en) 1990-09-07
JPH03501653A (en) 1991-04-11
FI905350A0 (en) 1990-10-30

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