KR920019926A - Semi-permeable membrane gel for cell culture and preparation method thereof - Google Patents

Semi-permeable membrane gel for cell culture and preparation method thereof Download PDF

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KR920019926A
KR920019926A KR1019910005802A KR910005802A KR920019926A KR 920019926 A KR920019926 A KR 920019926A KR 1019910005802 A KR1019910005802 A KR 1019910005802A KR 910005802 A KR910005802 A KR 910005802A KR 920019926 A KR920019926 A KR 920019926A
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semi
polysaccharide
gel
polymer
permeable membrane
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KR1019910005802A
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Korean (ko)
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KR930008121B1 (en
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이해방
이진호
박종우
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채영복
재단법인한국화학연구소
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means

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  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Water Supply & Treatment (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
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Abstract

내용 없음No content

Description

세포배양용 반투막겔과 그의 제조방법Semi-permeable membrane gel for cell culture and preparation method thereof

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (8)

매트릭스 내에서 세포를 고정화하여 고밀도로 배양할 수 있도록 된 반투막겔에 있어서, 다당류 또는 그 유도체와 분자량 10,000 이상의 수용성 관능기를 갖는 고분자가 1 : 4 내지 4 : 1 부피비율로 혼합된 상태의 매트릭스로 제조된 세포배양용 반투막겔.In the semipermeable membrane gel, which is capable of culturing cells at a high density by immobilizing cells in a matrix, a polysaccharide or a derivative thereof and a polymer having a water-soluble functional group having a molecular weight of 10,000 or more are prepared as a matrix in a 1: 4 to 4: 1 volume ratio. Semipermeable gel for cell culture. 제 1항에 있어서, 상기 다당류 또는 그 유도체와 상기 고분자의 혼합량은 전체 겔 조성의 0.5~10%의 농도인 것을 특징으로 하는 반투막겔.The semipermeable membrane gel according to claim 1, wherein the mixed amount of the polysaccharide or its derivatives and the polymer is 0.5 to 10% of the total gel composition. 제 1항 또는 제 2항에 있어서, 상기 다당류 또는 그 유도체는 일긴산, 소디움알기네이트, 카르복시메틸알긴산염, 펙틴, 카라기난, 키틴 또는 키토산, 잔탄검, 구아검, 아라비아검, 전분 또는 이들을 금속화물과 쉽게 킬레이트될 수 있도록 변성시킨 것들 중에서 선택된 것임을 특징으로 하는 반투막겔.The method according to claim 1 or 2, wherein the polysaccharide or derivatives thereof are monoginic acid, sodium alginate, carboxymethyl alginate, pectin, carrageenan, chitin or chitosan, xanthan gum, guar gum, arabic gum, starch or metalloids thereof. Semi-permeable membrane, characterized in that selected from among those modified to be easily chelate. 제 1항에 있어서, 상기 수용성 관능기를 갖는 고분자는 폴리비닐알코올, 폴리아크릴아미이드 또는 폴리아크릴산인 것을 특징으로 하는 반투막겔.The semipermeable membrane gel according to claim 1, wherein the polymer having a water-soluble functional group is polyvinyl alcohol, polyacrylamide or polyacrylic acid. 매트릭스 내에 세포를 고정화하여 배양시키도록 된 반투막겔을 제조함에 있어서, 다당류 또는 그 유도체와 분자량 10,000 이상의 수용성 관능기를 갖는 고분자를 1 : 4 내지 4 : 1로 혼합시켜 이 혼합량이 전체 겔 조성의 0.5~10% 농도가 되도록 다가 양이온 용액 중에서 가교시키는 것을 특징으로 하는 세포배양용 반투막겔의 제조방법.In preparing a semipermeable membrane gel in which cells are fixed and cultured in a matrix, a polysaccharide or a derivative thereof and a polymer having a water-soluble functional group having a molecular weight of 10,000 or more are mixed in a range of 1: 4 to 4: 1, and the mixing amount is 0.5 to Method for producing a semi-permeable membrane for cell culture, characterized in that the cross-linking in a polyvalent cation solution to 10% concentration. 제 1항에 있어서, 상기 다당류 또는 그 유도체로서는 알긴산, 소듐알기네이트, 카르복시메틸알긴산염, 펙틴, 카라기난, 키틴 또는 키토산, 잔탄검, 구아검, 아라비아검, 전분 또는 이들은 금속화물과 쉽게 킬레이트 될 수 있도록 변성시킨 것들 중에서 선택된 것을 사용함을 특징으로 하는 반투막겔의 제조방법.The method of claim 1, wherein the polysaccharide or derivatives thereof include alginic acid, sodium alginate, carboxymethyl alginate, pectin, carrageenan, chitin or chitosan, xanthan gum, guar gum, gum arabic, starch or they can be easily chelated with metallized compounds. Method for producing a semi-permeable membrane, characterized in that selected from those denatured to use. 제 1항에 있어서, 상기 수용성 관능기를 갖는 고분자로서는 폴리비닐알코올, 폴리아크릴아마이드 또는 폴리아크릴산을 사용함을 특징으로 하는 반투막겔의 제조방법.The method for producing a semipermeable membrane gel according to claim 1, wherein polyvinyl alcohol, polyacrylamide or polyacrylic acid is used as the polymer having a water-soluble functional group. 제 1항에 있어서, 상기 다가양이온 용액으로는 배양액에 CaCl2, SrCl2, BaCl2또는 AlCl3가 0.5~2.0% 농도로 용해되어 있는 용액을 사용하는 것을 특징으로 하는 반투막겔의 제조방법.The method of claim 1, wherein the polycationic solution is a solution of CaCl 2 , SrCl 2 , BaCl 2 or AlCl 3 dissolved in a concentration of 0.5 to 2.0% of the method for producing a semi-permeable membrane gel. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910005802A 1991-04-11 1991-04-11 Process for preparation of gel KR930008121B1 (en)

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KR1019910005802A KR930008121B1 (en) 1991-04-11 1991-04-11 Process for preparation of gel

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101665918B1 (en) * 2016-07-19 2016-10-12 아주대학교산학협력단 Method of manufacturing a polyvinyl alcohol nanofiber structure for cell culture

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107496975B (en) * 2017-08-14 2020-08-28 浙江大学 Polyvinyl alcohol/sodium alginate drug-loaded hydrogel dressing with photoresponsive antibacterial function and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101665918B1 (en) * 2016-07-19 2016-10-12 아주대학교산학협력단 Method of manufacturing a polyvinyl alcohol nanofiber structure for cell culture
WO2018016701A1 (en) * 2016-07-19 2018-01-25 아주대학교산학협력단 Method for producing polyvinyl alcohol nanofiber structure for cell culture
US11952687B2 (en) 2016-07-19 2024-04-09 NANOFAENTECH CO., Ltd. Method for producing cell-culturing polyvinyl alcohol nanofiber structure

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