KR920004574A - Method for producing human alpha interferon by genetic recombination - Google Patents

Method for producing human alpha interferon by genetic recombination Download PDF

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KR920004574A
KR920004574A KR1019900013738A KR900013738A KR920004574A KR 920004574 A KR920004574 A KR 920004574A KR 1019900013738 A KR1019900013738 A KR 1019900013738A KR 900013738 A KR900013738 A KR 900013738A KR 920004574 A KR920004574 A KR 920004574A
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iii
coli
recombinant
pif
human alpha
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KR1019900013738A
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KR930001387B1 (en
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최윤희
이재호
박형환
권익부
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정보영
롯데제과 주식회사
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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Abstract

내용 없음No content

Description

유전자 재조합에 의한 인간 알파인터페론의 제조방법Method for producing human alpha interferon by genetic recombination

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명에 사용된 벡터 플라스미드 pIN-Ⅲ-B3의 유전자 지도,1 is a genetic map of the vector plasmid pIN-III-B3 used in the present invention,

제2도는 벡터 플라스미드 pIN-Ⅲ-B3에 인간 알파유전자를 삽입하는 방법을 나타낸 개략도,2 is a schematic diagram showing how to insert a human alpha gene into the vector plasmid pIN-III-B3,

제3도는 인간 알파유전자가 삽입된 재조합 플라스미드Figure 3 shows recombinant plasmid with human alpha gene inserted

Claims (7)

공여 플라스미드 Hif-2H로부터 인간 알파인터페론 유전자를 분리하고, 이를 벡타 플라스미드 pIF-Ⅲ-B3에 삽입하여, 재조합 플라스미드 pIN-Ⅲ-B3를 제조한다음, 이를 대장균 JE5505에 형질전환시켜서, 재조합 대장균 JE5505(pIF-Ⅲ-B3)를 제조하고, 재조합 대장균 JE5505(pIF-Ⅲ-B3)를 고농도 세포배양하여 그로부터 인간 알파인터페론을 분리 정제하는 것을 특징으로 하는 인간 알파인터페론의 제조방법.The human alphainterferon gene was isolated from the donor plasmid Hif-2H, inserted into the vector plasmid pIF-III-B3 to prepare a recombinant plasmid pIN-III-B3, and then transformed into E. coli JE5505, and recombinant E. coli JE5505 ( pIF-III-B3), and cultured recombinant E. coli JE5505 (pIF-III-B3) in a high concentration cell culture method for the production of human alpha interferon characterized in that the separation of human alpha interferon therefrom. 제1항에 있어서, 상기 대장균 JE5505는 아미노산 요구성을 유전적 특징으로 가지는 것을 사용함을 특징으로 하는 인간 알파인터페론의 제조방법.The method of claim 1, wherein the E. coli JE5505 has an amino acid requirement as a genetic feature. 제1항에 있어서, 세포배양시에 외부로부터 카사미노 액시드의 공급속도를 조절하므로서 재조합 대장균 JE5505(pIF-Ⅲ-B3)의 성장속도를 제어하는 것을 특징으로 하는 인간 알파인터페론의 제조방법.The method for producing human alpha interferon according to claim 1, wherein the growth rate of recombinant E. coli JE5505 (pIF-III-B3) is controlled by controlling the feed rate of casamino acid from the outside during cell culture. 제1항에 있어서, 벡터 플라스미드 pIF-Ⅲ-B3는 분비형 벡터 플라스미드를 사용하므로서 제조된 인간 알파인터페론의 90%이상이 대장균의 페리플라스믹 공간에 존재하도록하는 것을 특징으로 하는 인간 알파인터페론의 제조방법.The method of claim 1, wherein the vector plasmid pIF-III-B3 is a human alpha interferon prepared by using a secretion-type vector plasmid so that at least 90% of the human alpha interferon is present in the periplasmic space of E. coli Way. 공여 플라스미드 Hif-2H로부터 인간 알파인터페론 유전자를 분리하고, 이를 벡타 플라스미드 pIN-Ⅲ-B3에 삽입하여서 제조된 재조합 플라스키드 pIF-Ⅲ-B3.The recombinant plasmid pIF-III-B3 prepared by separating the human alpha interferon gene from the donor plasmid Hif-2H and inserting it into the vector plasmid pIN-III-B3. 인간 인터페론 유전자가 삽입되어 있는 재조합 플라스미드 pIF-Ⅲ-B3로 형질전환된 것을 특징으로 하는 재조합 대장균 JE5505(KFCC 10704).Recombinant Escherichia coli JE5505 (KFCC 10704), which is transformed with the recombinant plasmid pIF-III-B3 into which a human interferon gene is inserted. 제6항에 있어서, 상기 재조합 대장균 JE5505(KFCC 10704)는 아미노산 요구성을 유전적 특징으로 가지는 것.7. The recombinant E. coli JE5505 (KFCC 10704) has an amino acid requirement as a genetic feature. ※참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is to be disclosed based on the initial application.
KR1019900013738A 1990-08-31 1990-08-31 Spinal corrctive tool method for preparation of alpha-interferon KR930001387B1 (en)

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KR1019900013738A KR930001387B1 (en) 1990-08-31 1990-08-31 Spinal corrctive tool method for preparation of alpha-interferon

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