KR920000943A - Natural-state methods and systems for measuring viability and proliferation of in vitro tissue - Google Patents

Abstract

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Description

Natural-state methods and systems for measuring viability and proliferation of in vitro tissue

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Claims (11)

a) tumor tissue and similar normal tissue samples are tissue cultured in separate containers in the first and second compartments; b) exposing the sample to a medicament having a tumor specific effect on proliferation and viability; c) treating said sample of said first compartment with a DNA-synthetic marker and said sample of said second compartment with a protein-synthetic marker; d) incubating the treated compartment of the sample for a predetermined period; e) measuring the amount of DNA-synthetic markers and protein-synthetic markers incorporated in the first and second compartments, respectively, to determine tumor specific effects on cell proliferation and viability of the compound. A method of measuring tumors in particular effects on proliferation and viability. The method of claim 1, wherein the DNA-synthesis-marker is ³ H-thymidine and the protein-synthesis-marker is ³⁵ S-methionine. The method of claim 1 wherein the predetermined period of step (d) is from 2 to about 5 days. The method of claim 1, wherein said first and second compartments of said sample are incubated for 2 to about 6 days prior to exposure of step (b). a) tissue culture of a sample of tumor cells; b) treating the sample with a DNA-synthetic marker; c) incubating the treated sample for a predetermined period; d) measuring the percentage of tumor cells having the DNA-synthetic marker incorporated into the cell, thereby measuring the aggressiveness of the tumor. The method of claim 5, wherein said DNA-synthetic marker is ³ H-deoxythymidine. A kit type diagnostic system for analyzing the viability and proliferation of cells from explanted tissues, characterized in that it contains a protein synthesis marker and a DNA synthesis marker in an amount sufficient for at least one assay. 8. The diagnostic system of claim 7, wherein said body sample is normal tissue or tumor tissue. 8. The diagnostic system of claim 7, wherein said protein synthesis marker is ³⁵ S-methionine and said DNA synthesis marker is ³ H-deoxythymidine. 8. The diagnostic system of claim 7, further comprising a predetermined amount of a medicament sufficient to affect cell viability or proliferation of the grafted tissue when the tissue is cultured in the presence of the medicament. The diagnostic system of claim 10, wherein the medicament is a tumor therapeutic. ※ Note: The disclosure is based on the initial application.