KR910012239A - Recombinant DNA molecule conferring pest resistance to plants and plant production method using same - Google Patents

Recombinant DNA molecule conferring pest resistance to plants and plant production method using same Download PDF

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KR910012239A
KR910012239A KR1019890019844A KR890019844A KR910012239A KR 910012239 A KR910012239 A KR 910012239A KR 1019890019844 A KR1019890019844 A KR 1019890019844A KR 890019844 A KR890019844 A KR 890019844A KR 910012239 A KR910012239 A KR 910012239A
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dna molecule
recombinant dna
gene sequence
plant
sequence encoding
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KR1019890019844A
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KR920002313B1 (en
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이종섭
문용호
백운화
최철희
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이종범
두산농산 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Plant Pathology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

내용 없음No content

Description

식물에 해충 내성을 부여하는 재조합 DNA분자 및 이를 이용한 식물 제조방법Recombinant DNA molecule conferring pest resistance to plants and plant production method using same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (15)

식물이 키모트립신 억제제를 생산하게 하여 식물에 해충내성을 부여하는 재조합 DNA분자.Recombinant DNA molecule that causes plants to produce chymotrypsin inhibitors, thereby imparting pest resistance to the plants. 제1항에 있어서, 키모트립신 억제제를 암호화하는 유전자 서열을 갖는 재조합 DNA분자.The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule has a gene sequence encoding a chymotrypsin inhibitor. 제1항에 있어서, 유전자 서열이 식물 세포내에서 발현되는 재조합 DNA분자.The recombinant DNA molecule of claim 1, wherein the gene sequence is expressed in plant cells. 제2항에 있어서, 키모트립신 억제제를 암호화하는 유전자 서열이 식물에서 유래한 재조합 DNA분자.The recombinant DNA molecule of claim 2, wherein the gene sequence encoding the chymotrypsin inhibitor is derived from a plant. 제2항에 있어서, 키모트립신 억제제를 암호화하는 유전자 서열이 토마토로 부터 유래한 재조합 DNA분자.The recombinant DNA molecule of claim 2, wherein the gene sequence encoding the chymotrypsin inhibitor is derived from tomatoes. 제5항에 있어서, 토마토의 키모트립신 억제제를 암호화하는 유전자 서열이 하기 뉴클레오티드 염기서열 뿐 아니라 이의 변이체 및 변종으로서 키모트립신 억제제 활성을 갖는 폴리펩티드를 암호화하는 유전자 서열을 포함하는 재조합 DNA분자.The recombinant DNA molecule of claim 5, wherein the gene sequence encoding the chymotrypsin inhibitor of tomato comprises a nucleotide sequence as well as a gene sequence encoding a polypeptide having chymotrypsin inhibitor activity as variants and variants thereof. 제1항 내지 제5항중 어느 하나에 있어서, 유전자 서열이 게놈 DNA, cDNA 또는 합성 DNA로 구성된 재조합 DNA분자.The recombinant DNA molecule according to any one of claims 1 to 5, wherein the gene sequence consists of genomic DNA, cDNA, or synthetic DNA. 기증적으로 식물 프로모터에 연결된 키모트립신 억제제 폴리펩타이드를 암호화하는 유전자 서열을 포함하는 제조합 DNA분자.A synthetic DNA molecule comprising a gene sequence encoding a chymotrypsin inhibitor polypeptide that is donated to a plant promoter. 제8항에 있어서, 유전자 서열이 식물 프로모터와 헤테로인 재조합 DNA분자.The recombinant DNA molecule of claim 8, wherein the gene sequence is heterologous to a plant promoter. 제8항에 있어서, 유전자 서열이 식물세포내에서 발현되는 재조합 DNA분자.The recombinant DNA molecule of claim 8, wherein the gene sequence is expressed in plant cells. 제10항에 있어서, 발현 시그널이 식물 또는 식물 비루스의 유전자로 부터 유래하는 재조합 DNA분자.The recombinant DNA molecule of claim 10, wherein the expression signal is derived from a gene of a plant or plant virus. 제11항에 있어서, 식물 비루스가 꽃양배추 모자이크 비루스인 재조합 DNA분자.The recombinant DNA molecule of claim 11, wherein the plant virus is Cauliflower mosaic virus. 제12항에 있어서, CaMV게놈의 35S 발현시그널을 포함하는 DNA분자.13. The DNA molecule of claim 12 comprising a 35S expression signal of CaMV genome. Vir 폴라스미드 pJSL231(KCTC 8478P).Vir Polismid pJSL231 (KCTC 8478P). 플라스미드 pJSL231(KCTC 8478P) 및 T-DNA 플라스미드를 포함하는 아그로박테리움 류메파시엔스의 형질전환계를 이용한 해충내성 식물 제조방법.A method for producing a pest-tolerant plant using a transformation system of Agrobacterium rhemefaciens comprising plasmid pJSL231 (KCTC 8478P) and T-DNA plasmid. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890019844A 1989-12-28 1989-12-28 Proteinase inhibitor gene KR920002313B1 (en)

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Application Number Priority Date Filing Date Title
KR1019890019844A KR920002313B1 (en) 1989-12-28 1989-12-28 Proteinase inhibitor gene

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Application Number Priority Date Filing Date Title
KR1019890019844A KR920002313B1 (en) 1989-12-28 1989-12-28 Proteinase inhibitor gene

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KR910012239A true KR910012239A (en) 1991-08-07
KR920002313B1 KR920002313B1 (en) 1992-03-21

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