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Application filed by 안시환, 제일제당 주식회사filedCritical안시환
Priority to KR1019890019746ApriorityCriticalpatent/KR910011280A/en
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Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
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알칼리성 포스파타제의 정제방법Method for Purifying Alkaline Phosphatase
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.
Claims (3)
포유동물의 내부 조직으로부터 알칼리성 포스파타제를 순수정제함에 있어서, 면역친화성 컬럼과 친화성 컬럼을 연속적으로 수행함을 특징으로하는 알칼리성 포스파타제의 정제방법.A method for purifying alkaline phosphatase, wherein the purification of alkaline phosphatase from internal tissues of a mammal is carried out successively with an affinity column and an affinity column.제1항에 있어서, 면역친화성 컬럼은 항알칼리성포스파타제 이뮤노글로부린(Anti-APase polyclonal IgG)를 Affi-Gel 10에 결합시킨 컬럼임을 특징으로하고, 용출은 PH 11-12, 인산염(Na2HPO4) 10mM-50mM에 염화나트륨(NaC1) 100mM-100mM를 포함한완충용액으로 수행함을 특징으로하는 알칼리성포스파타제의 정제방법.The method of claim 1, wherein the immuno-affinity column is characterized in that the anti-alkaline phosphatase immunoglobulin (Anti-APase polyclonal IgG) bound to Affi-Gel 10, elution is PH 11-12, phosphate (Na 2 HPO 4 ) Purifying alkaline phosphatase characterized in that it is carried out with a buffer solution containing 10mM-50mM sodium chloride (NaC1) 100mM-100mM.제1항에 있어서, 친화성컬럼은 L-히스티딜리아조벤질포스포닉산 아가로스(L-Hstidyldiazobenzylposphonic acid Agarose)를 사용하고, 용출은 PH7.0-0.9, 농도 10mM-50mM인 트리스 염산용액에 인산염(Na2PHO4) 10mM-30mM을 첨가한 완충용액으로 수행함을 특징으로하는 알칼리성포스파타제의 정제방법.The method of claim 1, wherein the affinity column is L-Hstidyldiazobenzylposphonic acid Agarose (L-Hstidyldiazobenzylposphonic acid Agarose), the elution is PH7.0-0.9, concentration of 10mM-50mM in Tris hydrochloric acid solution A method for purifying alkaline phosphatase, characterized in that it is carried out with a buffer solution containing 10 mM-30 mM of phosphate (Na 2 PHO 4 ).※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890019746A1989-12-271989-12-27
Method for Purifying Alkaline Phosphatase
KR910011280A
(en)
Method for the separation of lp(a) by electrophoresis and its application to the in vitro determination of atherogenic risk linked to the presence of lp(a)
AN INVESTIGATION OF THE SPECIFICITY OF ANTIBODIES RAISED TO THE CROTOXIN COMPLEX (FROM THE VENOM OF CROTALUS DURZSSUS TERRIFICUS) USING THE~ 1ETHODS OF DOUBLE-IP84UNODIFFUSION AND THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
Test method for determining phosphatase labeling toxins that inhibit protein phosphatases, detection kit for phosphatase labeling toxins, and use of the kit.