KR900009973A - Vector containing human growth hormone gene [pBR-HGH] and its manufacturing method - Google Patents

Vector containing human growth hormone gene [pBR-HGH] and its manufacturing method Download PDF

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Publication number
KR900009973A
KR900009973A KR1019880018189A KR880018189A KR900009973A KR 900009973 A KR900009973 A KR 900009973A KR 1019880018189 A KR1019880018189 A KR 1019880018189A KR 880018189 A KR880018189 A KR 880018189A KR 900009973 A KR900009973 A KR 900009973A
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South Korea
Prior art keywords
growth hormone
human growth
hgh
pbr
gene
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KR1019880018189A
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Korean (ko)
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KR910000456B1 (en
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조중명
이용범
이태규
박영우
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허신구
주식회사 럭 키
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Priority to KR1019880018189A priority Critical patent/KR910000456B1/en
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Publication of KR910000456B1 publication Critical patent/KR910000456B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

Abstract

내용 없음No content

Description

인간성장 호르몬 유전자를 함유하는 벡터[pBR-HGH]와 그의 제조방법Vector containing human growth hormone gene [pBR-HGH] and its manufacturing method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 인간성장호르몬의 cDNA 염기서열 및 아미노산 서열을 나타낸 것이고, 제2도는 본 발명에 따른 인간성장호르몬의 유전자를 함유하는 벡터[pBR-HGH]의 제조과정을 나타낸 것이며, 제3도는 본 발명에 따른 인간성장호르몬 유전자를 함유하는 [pBR-HGH]를 도시한 것이다.Figure 1 shows the cDNA base sequence and amino acid sequence of human growth hormone, Figure 2 shows the manufacturing process of the vector [pBR-HGH] containing the gene of human growth hormone according to the present invention, Figure 3 is the present invention It shows [pBR-HGH] containing the human growth hormone gene according to.

Claims (6)

제한효소 SstI 및 SaII 부위를 갖는 인간성장호르몬 유전자와 아미노말단 합성유전자 및 제한효소 NdeI/SaII으로 절단시킨 대장균 운반체로 구성되어진 것임을 특징으로 하는 인간성장호르몬 유전자를 함유하는 벡터[pBR-HGH].Vector [pBR-HGH] comprising a human growth hormone gene having restriction enzymes SstI and SaII sites, and an E. coli carrier digested with an amino-terminal synthetic gene and restriction enzyme NdeI / SaII. 제1항에 있어서, 인간성장호르몬 유전자는 인간성장호르몬의 16,17,18번째인 아미노산 위치에 제한효소 SstI 부위를 갖도록 하고 카르복시말단 위치에 제한효소 SaII 부위를 갖도록 제조된 것임을 특징으로 하는 인간성장호르몬 유전자를 함유하는 벡터[pBR-HGH].The human growth hormone gene according to claim 1, wherein the human growth hormone gene is prepared to have a restriction enzyme SstI site at the amino acid positions 16, 17, and 18 of the human growth hormone and to have a restriction enzyme SaII site at the carboxy terminal position. Vector containing hormonal genes [pBR-HGH]. 제1항에 있어서, 아미노 말단 합성 유전자는 윗가닥 55mer(5'-TATG TTC CCA ACC ATC CCA CTG TCT CGT CTG TTC GAC AAC GCT ATG CTG CGA GCT-3')와 아래가닥 49mer(3'-AC AAG GGT TGG TAG GGT GAC AGA GCA GAC AAG CTG TTG CGA TAC GAC GC-5')로 구성된 것임을 특징으로 하는 인간성장호르몬 발현 벡터[pBR-HGH].The amino terminus synthetic gene of claim 1, wherein the amino terminus synthetic gene has a top strand of 55mer (5′-TATG TTC CCA ACC ATC CCA CTG TCT CGT CTG TTC GAC AAC GCT ATG CTG CGA GCT-3 ′) and a bottom strand 49mer (3′-AC AAG). GGT TGG TAG GGT GAC AGA GCA GAC AAG CTG TTG CGA TAC GAC GC-5 ') human growth hormone expression vector, characterized in that consisting of [pBR-HGH]. cDNA 라이브러리에서 인간성장호르몬의 염기서열을 확인하여 제한효소 SstI 및 SaII 부위를 갖는 인간성장호르몬 유전자를 제조한 다음, 이 유전자를 아미노말단 합성유전자와 접합시키고 제한효소 NdeI/SaII으로 절단시킨 운반체에 클로닝시키는 것을 특징으로 하는 인간성장호르몬 유전자를 함유하는 백터[pBR-HGH]제조방법.The nucleotide sequence of human growth hormone was identified in the cDNA library to prepare a human growth hormone gene having restriction enzymes SstI and SaII sites, which was then conjugated with an amino-terminal synthetic gene and cloned into a carrier digested with restriction enzyme NdeI / SaII. Vector [pBR-HGH] manufacturing method containing a human growth hormone gene, characterized in that 제4항에 있어서, 인간성장호르몬 유전자는 인간성장호르몬의 16, 17, 18번째인 아미노산 위치에 제한효소 SstI 부위를 갖도록 하고 카르복시말단 위치에 제한효소 SaII 부위를 갖도록 제조된 것임을 특징으로 하는 인간 성장호르몬 유전자를 함유하는 벡터[pBR-HGH] 제조방법.The human growth hormone gene according to claim 4, wherein the human growth hormone gene is prepared to have a restriction enzyme SstI site at the 16th, 17th and 18th amino acid positions of the human growth hormone and to have a restriction enzyme SaII site at the carboxy terminal position. Method for producing a vector [pBR-HGH] containing a hormone gene. 제4항에 있어서, 아미노 말단 합성 유전자는 윗가닥 55mer(5'-TATG TTC CCA ACC ATC CCA CTG TCT CGT CTG TTC GAC AAC GCT ATG CTG CGA GCT-3')와 아래가닥 49mer(3'-AC AAG GGT TGG TAG GGT GAC AGA GCA GAC AAG CTG TTG CGA TAC GAC GC-5')로 구성된 것임을 특징으로 하는 인간성장호르몬 유전자를 함유하는 벡터[pBR-HGH] 제조방법.5. The amino terminus synthetic gene of claim 4, wherein the amino terminus synthetic gene has a top strand of 55mer (5'-TATG TTC CCA ACC ATC CCA CTG TCT CGT CTG TTC GAC AAC GCT ATG CTG CGA GCT-3 ') and a bottom strand 49mer (3'-AC AAG). GGT TGG TAG GGT GAC AGA GCA GAC AAG CTG TTG CGA TAC GAC GC-5 '), comprising a vector containing the human growth hormone gene [pBR-HGH] method. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019880018189A 1988-12-31 1988-12-31 Vector por-high for human growth hormone KR910000456B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019880018189A KR910000456B1 (en) 1988-12-31 1988-12-31 Vector por-high for human growth hormone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019880018189A KR910000456B1 (en) 1988-12-31 1988-12-31 Vector por-high for human growth hormone

Publications (2)

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KR900009973A true KR900009973A (en) 1990-07-06
KR910000456B1 KR910000456B1 (en) 1991-01-25

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