KR900001844A - Cyclodextrin-producing enzymes and their producing microorganisms - Google Patents

Cyclodextrin-producing enzymes and their producing microorganisms Download PDF

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Publication number
KR900001844A
KR900001844A KR1019880009365A KR880009365A KR900001844A KR 900001844 A KR900001844 A KR 900001844A KR 1019880009365 A KR1019880009365 A KR 1019880009365A KR 880009365 A KR880009365 A KR 880009365A KR 900001844 A KR900001844 A KR 900001844A
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KR
South Korea
Prior art keywords
producing
cyclodextrin
enzymes
microorganisms
kfcc
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Application number
KR1019880009365A
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Korean (ko)
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KR900005532B1 (en
Inventor
유주현
정용운
권익부
이윤수
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유주현
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Priority to KR1019880009365A priority Critical patent/KR900005532B1/en
Publication of KR900001844A publication Critical patent/KR900001844A/en
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Publication of KR900005532B1 publication Critical patent/KR900005532B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음.No content.

Description

사이클로 덱스트린(cyclodextrin) 생성 효소와 그의 생산 미생물Cyclodextrin-producing enzymes and their producing microorganisms

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 전분용액에 본 균주의 효소를 작용시켜 얻어진 반응 생성물을 HPLC(High Performance Liquid Chromatography) 분석한 도면이다.1 is a diagram of HPLC (High Performance Liquid Chromatography) analysis of the reaction product obtained by applying the enzyme of the strain to the starch solution.

제2도는 사이클로 덱스트린 생성효소를 생산하는 미생물의 전자 현미경 사진 도해이다.Figure 2 is an electron micrograph of microorganisms producing cyclodextrin synthase.

제3도는 정제된 효소 단백질의 에스디에스 폴리아크릴 아마이드 젤 전기 영동(SDS-Polyacrylamidegel electrophoresis) 사진이다.3 is a photograph of SDS-Polyacrylamide gel electrophoresis of purified enzyme protein.

제4도는 효소 단백질의 콜리아크릴 아마이드 젤 전기 영동(Polyacrylamide gel electrophoresis)을 행한 후 전분용액에 반응시켜 활성이 있는 위치를 확인한 결과이다.4 is a result of confirming the active position by reacting with starch solution after performing polyacrylamide gel electrophoresis of the enzyme protein.

Claims (2)

베타형 사이클로 덱스트린을 주로 생산하는 호알칼리성 바실러스 스페시스 KFCC 10623호 미생물.Alkaline Bacillus sp. KFCC 10623 microorganism mainly producing beta-type cyclodextrin. 최적 pH 6.0, 분자량 약 75000 달톤 크기의 단일 서브유니트를 가진 바실루스 스페시스 KFCC 10623호 유래의 사이클로 덱스트린 생성 효소.Cyclodextrin producing enzyme from Bacillus sp. KFCC 10623 having a single subunit of optimum pH 6.0, molecular weight about 75000 Daltons. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019880009365A 1988-07-26 1988-07-26 Cyclodextrin producing enzyme and micro-organism KR900005532B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019880009365A KR900005532B1 (en) 1988-07-26 1988-07-26 Cyclodextrin producing enzyme and micro-organism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019880009365A KR900005532B1 (en) 1988-07-26 1988-07-26 Cyclodextrin producing enzyme and micro-organism

Publications (2)

Publication Number Publication Date
KR900001844A true KR900001844A (en) 1990-02-27
KR900005532B1 KR900005532B1 (en) 1990-07-31

Family

ID=19276407

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880009365A KR900005532B1 (en) 1988-07-26 1988-07-26 Cyclodextrin producing enzyme and micro-organism

Country Status (1)

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KR (1) KR900005532B1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020038431A (en) * 2000-11-17 2002-05-23 김만순 V - eye clean
KR20020082193A (en) * 2002-10-08 2002-10-30 만 석 박 The medicine for the eyes and manufacturing method of that

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020038431A (en) * 2000-11-17 2002-05-23 김만순 V - eye clean
KR20020082193A (en) * 2002-10-08 2002-10-30 만 석 박 The medicine for the eyes and manufacturing method of that

Also Published As

Publication number Publication date
KR900005532B1 (en) 1990-07-31

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