KR900000483A - Chemiluminescence Measurement Method - Google Patents

Chemiluminescence Measurement Method Download PDF

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Publication number
KR900000483A
KR900000483A KR1019890008021A KR890008021A KR900000483A KR 900000483 A KR900000483 A KR 900000483A KR 1019890008021 A KR1019890008021 A KR 1019890008021A KR 890008021 A KR890008021 A KR 890008021A KR 900000483 A KR900000483 A KR 900000483A
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South Korea
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reaction
enzyme
measuring
carried out
terminator
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KR1019890008021A
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Korean (ko)
Inventor
마사히사 오가다
요시히로 아시하라
다다시 니노미야
아끼라 야노
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후꾸야마 가쓰
후지레비오 가부시기 가이샤
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Priority claimed from JP14151488A external-priority patent/JP2627308B2/en
Application filed by 후꾸야마 가쓰, 후지레비오 가부시기 가이샤 filed Critical 후꾸야마 가쓰
Publication of KR900000483A publication Critical patent/KR900000483A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Abstract

내용 없음No content

Description

화학발광 측정방법Chemiluminescence Measurement Method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 알칼리 조건에 따른 발광의 의존성을 보여주는 그래프이고,1 is a graph showing the dependence of luminescence according to alkaline conditions,

제2도는 효소반응을 효소저해제로 정지시킨 후에 알칼리 조건하에서 발광반응을 수행할 때에 얻어지는 발광의 프로파일을 보여주는 그래프이며,2 is a graph showing the profile of luminescence obtained when the luminescence reaction is carried out under alkaline conditions after stopping the enzyme reaction with an enzyme inhibitor.

제3도는 발광반응이 산의 첨가로 정지되고 그리고 이어서 알칼리 조건하에서 재개시될 때에 얻어지는 발광의 프로파일을 보여주는 그래프이다.3 is a graph showing the profile of luminescence obtained when the luminescence reaction is stopped by addition of acid and then resumed under alkaline conditions.

Claims (16)

pH 4 내지 10.5에서 효소로서 산 또는 알칼리 포르파타제 그리고 기질로서 다음 일반식(Ⅰ)의 디옥세탄 유도체를 사용하여 효소반응을 실시하고, 그리고 이어서 강 알칼리 조건에서 발광반응을 실시하는 것으로 되는 화학발광 측정방법.chemiluminescence, which is carried out at pH 4 to 10.5 using an acid or alkali porfatase as an enzyme and a dioxetane derivative of the following general formula (I) as a substrate, followed by a luminescence reaction under strong alkaline conditions. How to measure. 상기식에서, R은 저급알킬기이고, Ar은 방향족기이다.Wherein R is a lower alkyl group and Ar is an aromatic group. 제1항에 있어서, 측정방법을 효소면역법에 사용하는 측정방법.The measuring method according to claim 1, wherein the measuring method is used for enzyme immunoassay. 제1항에 있어서, 발광반응이 pH 11 내지 14에서 수행하는 측정방법.The method of claim 1, wherein the luminescence reaction is performed at pH 11-14. 제1항에 있어서, 측정방법을 폴리뉴클레오티드의 측정에 사용되는 측정방법.The method of claim 1, wherein the method of measurement is used for the measurement of a polynucleotide. 제1항 내지 제4항중 어느 하나에 있어서, 발광반응이 효소반응을 정지제의 첨가에 의해 정지된 후에 강알칼리 내에서 수행되는 측정방법.The measuring method according to any one of claims 1 to 4, wherein the luminescence reaction is carried out in strong alkali after the enzymatic reaction is stopped by the addition of a terminator. 제5항에 있어서, 정지제가 효소저해제 또는 산인 측정방법.The method of claim 5, wherein the terminator is an enzyme inhibitor or an acid. 제1항 내지 제4항중 어느 하나에 있어서, 엔헨서가 반응이 강 알칼리에서 수행될 때에도 존재하는 측정방법.The method according to any one of claims 1 to 4, wherein the enhancer is present even when the reaction is carried out in strong alkalis. 제7항에 있어서, 엔헨서가 포유류 혈청알부민, 폴리알킬사차아민, 플루오로세인 그리고/또는 디메틸술폭시드인 측정방법.8. The method of claim 7, wherein the enhancer is mammalian serum albumin, polyalkylschaamine, fluorosane and / or dimethylsulfoxide. pH 4 내지 10.5에서 효소에서 산 또는 알칼리 포스파타제 그리고 기질로서 다음 일반식(Ⅰ)의 디옥세탄 유도체를 사용하여 효소반응을 수행하고, 그리고 그후에 알칼리 조건하에서 효소반응계의 고체상만을 발광반응에 사용하는 것으로 되는 고체상을 사용하는 화학발광 측정방법.The enzyme reaction is carried out at pH 4 to 10.5 using an acid or alkaline phosphatase in the enzyme and a dioxetane derivative of the following general formula (I) as a substrate, and then only the solid phase of the enzyme reaction system is used for the luminescence under alkaline conditions. Chemiluminescence measurement method using a solid phase. 상기식에서, R은 저급알킬기이고, Ar은 방향족기이다.Wherein R is a lower alkyl group and Ar is an aromatic group. 제9항에 있어서, 측정방법을 효소 면역 측정에 사용하는 측정방법.The measuring method according to claim 9, wherein the measuring method is used for measuring enzyme immunity. 제9항에 있어서, 측정방법을 폴리뉴클레오티드의 측정에 사용하는 측정방법.The measuring method according to claim 9, wherein the measuring method is used for measuring a polynucleotide. 제9항 내지 제11항중 어느 하나에 있어서, 발광반응이 정지제의 첨가에 의해 효소반응이 정지된 후에 알칼리에서 수행하는 측정방법.The measurement method according to any one of claims 9 to 11, wherein the luminescence reaction is performed in alkali after the enzymatic reaction is stopped by addition of a terminator. 제12항에 있어서, 정지제가 효소 저해제 또는 산인 측정방법.The method of claim 12, wherein the terminator is an enzyme inhibitor or an acid. 제9항 내지 제11항중 어느 하나에 있어서, 엔헨서가 반응이 알칼리 중에서 수행될 때에도 존재하는 측정방법.The method according to any one of claims 9 to 11, wherein the enhancer is present even when the reaction is carried out in alkali. 제14항에 있어서, 엔헨서가 포유동물 혈청 알부민, 폴리알킬사차아민, 플루오레세인 그리고/또는 디메틸술폭시드인 측정방법.The method of claim 14, wherein the enhancer is mammalian serum albumin, polyalkylschaamine, fluorescein and / or dimethylsulfoxide. 제9항에 있어서, 고체상이 폴리스티렌, 폴리플루오로에틸렌, 나일론 또는 폴리아세탈인 측정방법.10. The method of claim 9, wherein the solid phase is polystyrene, polyfluoroethylene, nylon or polyacetal. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890008021A 1988-06-10 1989-06-10 Chemiluminescence Measurement Method KR900000483A (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP14151488A JP2627308B2 (en) 1988-06-10 1988-06-10 Chemiluminescent immunoassay using enzymes
JP63-141514 1988-06-10
JP17819388 1988-07-19
JP17819488 1988-07-19
JP63-178193 1988-07-19
JP63-178194 1988-07-19

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KR900000483A true KR900000483A (en) 1990-01-30

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0462224B1 (en) * 1989-03-10 1994-08-31 Millipore Corporation Sequencing nucleic acids using chemiluminescent detection

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AU3634089A (en) 1989-12-14

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